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Information on EC 3.4.11.19 - D-stereospecific aminopeptidase and Organism(s) Bacillus subtilis and UniProt Accession P26902

for references in articles please use BRENDA:EC3.4.11.19
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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.19 D-stereospecific aminopeptidase
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This record set is specific for:
Bacillus subtilis
UNIPROT: P26902 not found.
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Word Map
The taxonomic range for the selected organisms is: Bacillus subtilis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Release of an N-terminal D-amino acid from a peptide, Xaa-/-Yaa-, in which Xaa is preferably D-Ala, D-Ser or D-Thr. D-Amino acid amides and methyl esters also are hydrolysed, as is glycine amide
Synonyms
d-aminopeptidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D-aminopeptidase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
57534-78-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-Ala-4-nitroanilide + H2O
D-Ala + 4-nitroaniline
show the reaction diagram
-
-
-
?
D-Ala-D-Ala + H2O
D-Ala
show the reaction diagram
-
-
-
?
D-Ala-Gly-Gly + H2O
D-Ala + Gly-Gly
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
NaCl
1 M, 40% residual activity
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
pH 8.0, presence of ZnSO4
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
2% of maximum activity
7
20% of maximum activity
8
60% of maximum activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
isoelectric focusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
dppA gene product
Uniprot
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
230000
gel filtration
30184
8 * 31000, SDS-PAGE, 8 * 30184, calculated, 8 * 30909, mass spectrometry of His-tagged protein
30909
8 * 31000, SDS-PAGE, 8 * 30184, calculated, 8 * 30909, mass spectrometry of His-tagged protein
31000
8 * 31000, SDS-PAGE, 8 * 30184, calculated, 8 * 30909, mass spectrometry of His-tagged protein
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
octamer
8 * 31000, SDS-PAGE, 8 * 30184, calculated, 8 * 30909, mass spectrometry of His-tagged protein
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
10 min, stable
75
10 min, 20% residual activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cheggour, A.; Fanuel, L.; Duez, C.; Joris, B.; Bouillenne, F.; Devreese, B.; Van Driessche, G.; Van Beeumen, J.; Frere, J.M.; Goffin, C.
The dppA gene of Bacillus subtilis encodes a new D-aminopeptidase
Mol. Microbiol.
38
504-513
2000
Bacillus subtilis (P26902), Bacillus subtilis
Manually annotated by BRENDA team