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Information on EC 3.4.11.1 - leucyl aminopeptidase and Organism(s) Escherichia coli and UniProt Accession P68767

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.1 leucyl aminopeptidase
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This record set is specific for:
Escherichia coli
UNIPROT: P68767 not found.
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Word Map
The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid mides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
Synonyms
leucine aminopeptidase, erap2, leucyl aminopeptidase, leucine amino peptidase, l-leucine aminopeptidase, leucinaminopeptidase, pilsap, leucine aminopeptidase 3, leucylaminopeptidase, adipocyte-derived leucine aminopeptidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aminopeptidase A
-
Aminopeptidase A/I
-
-
-
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cathepsin III
-
-
-
-
cytosol aminopeptidase
-
-
-
-
DR57
-
-
-
-
FTBL proteins
-
-
-
-
L-leucine aminopeptidase
-
-
-
-
leucinamide aminopeptidase
-
-
-
-
leucinaminopeptidase
-
-
-
-
leucine aminopeptidase
Leucyl aminopeptidase
-
-
-
-
leucyl peptidase
-
-
-
-
PepA peptidase A
-
-
peptidase S
-
-
-
-
proline aminopeptidase
-
-
-
-
Prolyl aminopeptidase
-
-
-
-
proteins, specific or class, FTBL
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
show the reaction diagram
exoprotease, preference for N-terminal Leu, Met and Arg residues, low activity when Xaa is Gly, no activity when Xaa is Asp
-
PATHWAY SOURCE
PATHWAYS
CAS REGISTRY NUMBER
COMMENTARY hide
9001-61-0
-
90119-07-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ala-Gly + H2O
Ala + Gly
show the reaction diagram
-
low activity
-
?
Ala-Leu + H2O
Ala + Leu
show the reaction diagram
-
high activity
-
?
Arg-Ala + H2O
Arg + Ala
show the reaction diagram
-
-
-
?
Arg-Glu + H2O
Arg + Glu
show the reaction diagram
-
low activity
-
?
Arg-Gly + H2O
Arg + Gly
show the reaction diagram
Arg-Gly-Arg + H2O
Arg + Gly-Arg
show the reaction diagram
-
low activity
-
?
Arg-Gly-Asp + H2O
Arg + Gly-Asp
show the reaction diagram
-
-
-
?
Arg-Gly-Gly + H2O
Arg + Gly-Gly
show the reaction diagram
-
-
-
?
Arg-Gly-Leu + H2O
Arg + Gly-Leu
show the reaction diagram
-
-
-
?
Arg-Gly-Phe + H2O
Arg + Gly-Phe
show the reaction diagram
-
-
-
?
Arg-Gly-Ser + H2O
Arg + Gly-Ser
show the reaction diagram
-
-
-
?
Arg-Ile + H2O
Arg + Ile
show the reaction diagram
-
-
-
?
Arg-Leu + H2O
Arg + Leu
show the reaction diagram
-
high activity
-
?
Arg-Phe + H2O
Arg + Phe
show the reaction diagram
-
-
-
?
Arg-Val + H2O
Arg + Val
show the reaction diagram
-
-
-
?
Cys-Gly + H2O
Cys + Gly
show the reaction diagram
-
low activity
-
?
Gly-Leu + H2O
Gly + Leu
show the reaction diagram
-
low activity
-
?
Gly-Phe + H2O
Gly + Phe
show the reaction diagram
-
low activity
-
?
Gly-Trp + H2O
Gly + Trp
show the reaction diagram
-
low activity
-
?
Gly-Tyr + H2O
Gly + Tyr
show the reaction diagram
-
low activity
-
?
His-Gly + H2O
His + Gly
show the reaction diagram
-
-
-
?
His-Leu + H2O
His + Leu
show the reaction diagram
-
low activity
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
show the reaction diagram
-
-
-
?
Leu-Arg + H2O
Leu + Arg
show the reaction diagram
-
-
-
?
Leu-Asn + H2O
Leu + Asn
show the reaction diagram
-
-
-
?
Leu-Asp + H2O
Leu + Asp
show the reaction diagram
-
low activity
-
?
Leu-Gly + H2O
Leu + Gly
show the reaction diagram
-
-
-
?
Leu-Gly-Gly + H2O
Leu + Gly-Gly
show the reaction diagram
-
-
-
?
Leu-Leu + H2O
Leu + Leu
show the reaction diagram
-
-
-
?
Leu-Met + H2O
Leu + Met
show the reaction diagram
-
-
-
?
Leu-Phe + H2O
Leu + Phe
show the reaction diagram
-
-
-
?
Leu-Ser + H2O
Leu + Ser
show the reaction diagram
-
-
-
?
Leu-Trp + H2O
Leu + Trp
show the reaction diagram
-
-
-
?
Leu-Tyr + H2O
Leu + Tyr
show the reaction diagram
-
-
-
?
Leu-Val + H2O
Leu + Val
show the reaction diagram
-
-
-
?
Met-Leu + H2O
Met + Leu
show the reaction diagram
-
-
-
?
Phe-Gly + H2O
Phe + Gly
show the reaction diagram
-
-
-
?
Phe-Leu + H2O
Phe + Leu
show the reaction diagram
-
high activity
-
?
Pro-Leu + H2O
Pro + Leu
show the reaction diagram
-
-
-
?
Thr-Leu + H2O
Thr + Leu
show the reaction diagram
-
-
-
?
Trp-Leu + H2O
Trp + Leu
show the reaction diagram
-
low activity
-
?
Tyr-Gly + H2O
Tyr + Gly
show the reaction diagram
-
-
-
?
Tyr-Leu + H2O
Tyr + Leu
show the reaction diagram
-
-
-
?
Val-Gly + H2O
Val + Gly
show the reaction diagram
-
-
-
?
Val-Leu + H2O
Val + Leu
show the reaction diagram
-
high activity
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
-
activates
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
196.7
-
purified enzyme, substrate Arg-Gly-Ser
300.1
-
purified enzyme, substrate Arg-Gly-Gly
318
-
purified enzyme, substrate Leu-Gly
355.1
-
purified enzyme, substrate Arg-Gly-Phe
396.3
-
purified enzyme, substrate Arg-Gly-Leu
443.1
-
purified enzyme, substrate Arg-Gly
604.7
-
purified enzyme, substrate Leu-Gly-Gly
68.7
-
purified enzyme, substrate Arg-Gly-Arg
790.5
-
purified enzyme, substrate Arg-Gly-Asp
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
trigger enzymes combine metabolic function with regulation of gene expression. Escherichia coli aminopeptidase A (PepA) is a trigger enzyme endowed with catalytic activity and DNA-binding properties prominent in transcriptional regulation and site-specific DNA recombination. PepA is a repressor in its own right, capable of specifically inhibiting transcription initiation at promoter P1 of the carAB operon, encoding carbamoylphosphate synthase. Furthermore, PepA binding constrains a single positive supercoil in the carP1 control region. Such a topological event is understood to constitute an impediment to transcription initiation and may serve as a mechanism to regulate gene expression. The observed inhibitory effect of PepA at carP1 is specific
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53500
-
x * 53500, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 53500, SDS-PAGE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene pepA, recombinant overexpression in Escherichia coli strain JM101
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gu, Y.Q.; Walling, L.L.
Specificity of the wound-induced leucine aminopeptidase (LAP-A) of tomato. Activity on dipeptide and tripeptide substrates
Eur. J. Biochem.
267
1178-1187
2000
Escherichia coli, Solanum lycopersicum, Sus scrofa
Manually annotated by BRENDA team
Nguyen Le Minh, P.; Nadal, M.; Charlier, D.
The trigger enzyme PepA (aminopeptidase A) of Escherichia coli, a transcriptional repressor that generates positive supercoiling
FEBS Lett.
590
1816-1825
2016
Escherichia coli (P68767)
Manually annotated by BRENDA team