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Information on EC 3.3.2.13 - chorismatase and Organism(s) Streptomyces hygroscopicus and UniProt Accession Q9KID9

for references in articles please use BRENDA:EC3.3.2.13
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EC Tree
     3 Hydrolases
         3.3 Acting on ether bonds
             3.3.2 Ether hydrolases
                3.3.2.13 chorismatase
IUBMB Comments
The enzyme found in several bacterial species is involved in the biosynthesis of macrocyclic polyketides.
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This record set is specific for:
Streptomyces hygroscopicus
UNIPROT: Q9KID9
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Word Map
The taxonomic range for the selected organisms is: Streptomyces hygroscopicus
The enzyme appears in selected viruses and cellular organisms
Synonyms
chorismatase, ch-fkbo, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
chorismate/3,4-dihydroxycyclohexa-1,5-dienoate synthase
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-
-
-
rapK
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
chorismate + H2O = (4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
chorismate pyruvate-hydrolase
The enzyme found in several bacterial species is involved in the biosynthesis of macrocyclic polyketides.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
chorismate + H2O
(4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate
show the reaction diagram
chorismate + H2O
3,4-dihydroxycyclohexa-1,5-dienecarboxylate + pyruvate
show the reaction diagram
-
strict preference for chorismate
-
-
?
additional information
?
-
-
isochorismate is not accepted as a substrate
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
chorismate + H2O
(4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate
show the reaction diagram
chorismate + H2O
3,4-dihydroxycyclohexa-1,5-dienecarboxylate + pyruvate
show the reaction diagram
-
strict preference for chorismate
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-(2-carboxyethyl)benzoate
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
FKBO_STRHY
344
0
36947
Swiss-Prot
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A244G
site-directed mutagenesis
V209Q
site-directed mutagenesis
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA affinity column chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hubrich, F.; Mueller, M.; Andexer, J.N.
In vitro production and purification of isochorismate using a two-enzyme cascade
J. Biotechnol.
191
93-98
2014
Streptomyces hygroscopicus
Manually annotated by BRENDA team
Zhang, Y.; Zhang, H.; Zheng, Q.
How chorismatases regulate distinct reaction channels in a single conserved active pocket mechanistic analysis with QM/MM (ONIOM) investigations
Chemistry
25
1326-1336
2019
Streptomyces hygroscopicus (Q9KID9)
Manually annotated by BRENDA team
Dong, L.; Liu, Y.
Comparative studies of the catalytic mechanisms of two chorismatases CH-fkbo and CH-Hyg5
Proteins
85
1146-1158
2017
Streptomyces hygroscopicus (Q9KID9), Streptomyces hygroscopicus
Manually annotated by BRENDA team