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Information on EC 3.2.2.3 - uridine nucleosidase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9SJM7

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EC Tree
     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.3 uridine nucleosidase
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Select one or more organisms in this record: ?
This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9SJM7 not found.
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
uridine hydrolase, uridine nucleosidase, inosine-uridine nucleoside hydrolase, uridine ribohydrolase, nucleoside hydrolase 1, urh1p, uridine-cytidine n-ribohydrolase, uridine nucleoside ribohydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleoside hydrolase 1
-
uridine-ribohydrolase 1
-
NSH1
-
isoform, former designation URH1
nucleosidase, uridine
-
-
-
-
uridine hydrolase
-
-
-
-
Uridine ribohydrolase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of N-glycosyl bond
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
uridine ribohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9025-47-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
uridine + H2O
D-ribose + uracil
show the reaction diagram
-
-
-
?
5-methyluridine + H2O
D-ribose + 5-methyluracil
show the reaction diagram
-
-
-
-
?
adenosine + H2O
D-ribose + adenine
show the reaction diagram
-
-
-
-
?
inosine + H2O
D-ribose + hypoxanthine
show the reaction diagram
-
-
-
-
?
isopentenyladenine riboside + H2O
D-ribose + N6-isopentenyladenine
show the reaction diagram
-
-
-
-
?
uridine + H2O
D-ribose + uracil
show the reaction diagram
-
isoform NSH1 shows highest hydrolysis capacity for uridine
-
-
?
xanthosine + H2O
D-ribose + xanthine
show the reaction diagram
-
-
-
-
?
additional information
?
-
the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8 - 2.1
uridine
0.44
5-methyluridine
-
at 30°C, pH not specified in the publication
0.7
adenosine
-
at 30°C, pH not specified in the publication
1.4
Inosine
-
at 30°C, pH not specified in the publication
0.8
uridine
-
at 30°C, pH not specified in the publication
1.69
Xanthosine
-
at 30°C, pH not specified in the publication
additional information
additional information
kinetics
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
303.3
purified recombinant enzyme, substrate uridine
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
malfunction
-
a knockout mutant for isoform NSH1 shows symptoms of accelerated senescence, accompanied by marked accumulation of uridine and xanthosine under conditions of prolonged darkness
physiological function
-
isoform NSH1 represents the leading activity in purine and pyrimidine breakdown in a cell
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
URH1_ARATH
336
0
36086
Swiss-Prot
other Location (Reliability: 2)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
mutants expressing the Arabidopsis thaliana enzyme or the homologue from Oryza sativa exhibit resistance toward toxic fluorouridine, fluorouracil, and fluoroorotic acid. Mutants with increased and decreased nucleosidase activity are delayed in germination
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
URH1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, functional complementation of a yeast mutant, expression in transgenic Arabidopsis thaliana mutant plants under control of the CaMV 35S promoter
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Jung, B.; Floerchinger, M.; Kunz, H.H.; Traub, M.; Wartenberg, R.; Jeblick, W.; Neuhaus, H.E.; Moehlmann, T.
Uridine-ribohydrolase is a key regulator in the uridine degradation pathway of Arabidopsis
Plant Cell
21
876-891
2009
Arabidopsis thaliana (Q9SJM7), Oryza sativa
Manually annotated by BRENDA team
Mach, J.
Uridine ribohydrolase and the balance between nucleotide degradation and salvage
Plant Cell
21
699
2009
Arabidopsis thaliana (Q9SJM7)
Manually annotated by BRENDA team
Jung, B.; Hoffmann, C.; Moehlmann, T.
Arabidopsis nucleoside hydrolases involved in intracellular and extracellular degradation of purines
Plant J.
65
703-711
2011
Arabidopsis thaliana
Manually annotated by BRENDA team
Baccolini, C.; Witte, C.P.
AMP and GMP catabolism in Arabidopsis converge on xanthosine, which is degraded by a nucleoside hydrolase heterocomplex
Plant Cell
31
734-751
2019
Arabidopsis thaliana (Q9SJM7), Arabidopsis thaliana
Manually annotated by BRENDA team