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Information on EC 3.2.2.3 - uridine nucleosidase and Organism(s) Saccharomyces cerevisiae and UniProt Accession Q04179

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EC Tree
     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.3 uridine nucleosidase
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: Q04179 not found.
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Word Map
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
uridine hydrolase, uridine nucleosidase, inosine-uridine nucleoside hydrolase, uridine ribohydrolase, nucleoside hydrolase 1, urh1p, uridine-cytidine n-ribohydrolase, uridine nucleoside ribohydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
uridine/cytidine hydrolase
-
nucleosidase, uridine
-
-
-
-
uridine hydrolase
Uridine ribohydrolase
-
-
-
-
uridine-cytidine N-ribohydrolase
-
-
uridine-preferring pyrimidine nucleoside hydrolase
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
uridine + H2O = D-ribose + uracil
show the reaction diagram
substrate binding site, active site structure, His254 is important, catalytic mechanism
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of N-glycosyl bond
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
uridine ribohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9025-47-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-fluorocytidine + H2O
D-ribose + 5-fluorocytosine
show the reaction diagram
-
-
-
?
5-fluorouridine + H2O
D-ribose + 5-fluorouracil
show the reaction diagram
-
-
-
?
cytidine + H2O
D-ribose + cytosine
show the reaction diagram
-
-
-
?
deoxycytidine + H2O
D-deoxyribose + cytosine
show the reaction diagram
-
-
-
?
uridine + H2O
D-ribose + uracil
show the reaction diagram
5-methyluridine + H2O
5-methyluracil + D-ribose
show the reaction diagram
-
-
-
-
?
cytidine + H2O
cytosine + D-ribose
show the reaction diagram
-
-
-
-
?
cytidine + H2O
D-ribose + cytosine
show the reaction diagram
-
-
-
-
?
nicotinamide riboside + H2O
nicotinamide + D-ribose
show the reaction diagram
-
the uridine hydrolase is 100fold more active as a nicotinamide riboside hydrolase than as a uridine hydrolase
-
-
?
nicotinic acid riboside + H2O
nicotinic acid + D-ribose
show the reaction diagram
-
-
-
-
?
nicotinic acid riboside methyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
uridine + H2O
D-ribose + uracil
show the reaction diagram
-
-
-
-
?
uridine + H2O
uracil + D-ribose
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
uridine + H2O
D-ribose + uracil
show the reaction diagram
enzyme is important for recycling, via the salvage pathway, pyrimidine deoxy- and ribonucleosides into uracil required for the growth of strains lacking de novo pyrimidine synthesis pathway
-
-
?
cytidine + H2O
D-ribose + cytosine
show the reaction diagram
-
-
-
-
?
uridine + H2O
D-ribose + uracil
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
copper
-
contains 1 copper atom per enzyme molecule
Cu2+
-
contains 1 gatom of Cu2+ per enzyme molecule
additional information
-
Urh1 activity is unaffected by the addition of MgCl2
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5-fluorocytidine
noncompetitive
5-fluorouridine
cytidine
noncompetitive
deoxycytidine
competitive
Inosine
noncompetitive
ribosylthymine
competitive
1,10-phenanthroline
-
-
D-ribose
glucose 6-phosphate
immucillin-H
-
-
ribose 5-phosphate
-
-
ribosylthymine
ribulose 5-phosphate
-
-
uridine
-
above 0.4 mM
additional information
-
Urh1 activity is unaffected by EDTA
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.66
5-methyluridine
-
-
0.016
nicotinamide riboside
-
in 50 mM Tris-HCl, pH 7.0
0.15
nicotinic acid riboside
-
in 50 mM Tris-HCl, pH 7.0
0.045
nicotinic acid riboside methyl ester
-
in 50 mM Tris-HCl, pH 7.0
0.86 - 1.6
uridine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
23.4
nicotinamide riboside
-
in 50 mM Tris-HCl, pH 7.0
1.8
nicotinic acid riboside
-
in 50 mM Tris-HCl, pH 7.0
2.3
nicotinic acid riboside methyl ester
-
in 50 mM Tris-HCl, pH 7.0
20
uridine
-
in 50 mM Tris-HCl, pH 7.0
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3
5-fluorocytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
0.6
5-fluorouridine
pH 7.5, 37°C, wild-type enzyme, versus uridine
2
cytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
3
deoxycytidine
pH 7.5, 37°C, wild-type enzyme, versus uridine
3
Inosine
pH 7.5, 37°C, wild-type enzyme, versus uridine
2
ribosylthymine
pH 7.5, 37°C, wild-type enzyme, versus uridine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
wild-type strain MATaa and mutant derivatives, gene URH1
SwissProt
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
16500
-
2 * 16500, SDS-PAGE
17000
-
2 * 17000, SDS-PAGE
30000
-
gel filtration
32500
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H254A
sited-directed mutagenesis, active site mutant, 30fold decreased Vmax and unaltered Km compared to the wild-type enzyme
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
-
10 min, stable up to
58
-
10 min, 40% loss of activity
65
-
10 min, complete inactivation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for several months
-
4°C, 100 mM phosphate buffer, pH 7.2, 50 mM Tris-HCl, pH 7.0, stable for up to 15 days
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
construction of enzyme overexpressing strains
-
expressed in Escherichia coli
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pharmacology
-
Saccharomyces cerevisiae enzyme deficient mutant strains expressing protozoan enzymes could be useful tools in drug screening for specific inhibitors against the pathogenic parasites
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Magni, G.
Uridine nucleosidase from yeast
Methods Enzymol.
51
290-296
1978
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Magni, G.; Natalini, P.; Ruggieri, S.; Vita, A.
Baker's yeast uridine nucleosidase is a regulatory copper containing protein
Biochem. Biophys. Res. Commun.
69
724-730
1976
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Vita, A.; Natalini, P.; Ipata, P.L.; Magni, G.
Baker's yeast uridine nucleosidase. II. Enzymatic and kinetic properties
Boll. Soc. Ital. Biol. Sper.
50
1077-1083
1974
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Corradetti, E.; Natalini, P.; Ipata, P.L.; Magni, G.
Baker's yeast uridine nucleosidase. I. Purification to homogeneity
Boll. Soc. Ital. Biol. Sper.
50
1070-1076
1974
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Magni, G.; Fioretti, E.; Ipata, P.L.; Natalini, P.
Bakers yeast uridine nucleosidase. Purification, composition, and physical and enzymatic properties
J. Biol. Chem.
250
9-13
1975
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Raggi-Ranieri, M.; Ipata, P.L.
Partial purification and properties of uridine nucleosidase from Baker's yeast
Ital. J. Biochem.
20
27-43
1971
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Kurtz, J.E.; Exinger, F.; Erbs, P.; Jund, R.
New insights into the pyrimidine salvage pathway of Saccharomyces cerevisiae: requirement of six genes for cytidine metabolism
Curr. Genet.
36
130-136
1999
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Mitterbauer, R.; Karl, T.; Adam, G.
Saccharomyces cerevisiae URH1 (encoding uridine-cytidine N-ribohydrolase): functional complementation by a nucleoside hydrolase from a protozoan parasite and by a mammalian uridine phosphorylase
Appl. Environ. Microbiol.
68
1336-1343
2002
Crithidia fasciculata (Q27546), Homo sapiens, Saccharomyces cerevisiae
Manually annotated by BRENDA team
Kurtz, J.E.; Exinger, F.; Erbs, P.; Jund, R.
The URH1 uridine ribohydrolase of Saccharomyces cerevisiae
Curr. Genet.
41
132-141
2002
Saccharomyces cerevisiae (Q04179), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Belenky, P.; Christensen, K.C.; Gazzaniga, F.; Pletnev, A.A.; Brenner, C.
Nicotinamide riboside and nicotinic acid riboside salvage in fungi and mammals. Quantitative basis for Urh1 and purine nucleoside phosphorylase function in NAD+ metabolism
J. Biol. Chem.
284
158-164
2009
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4742
Manually annotated by BRENDA team