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Information on EC 3.2.2.29 - thymine-DNA glycosylase

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EC Tree
     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.29 thymine-DNA glycosylase
IUBMB Comments
Thymine-DNA glycosylase is part of the DNA-repair machinery. Thymine removal is fastest when it is from a G/T mismatch with a 5'-flanking C/G pair. The glycosylase removes uracil from G/U, C/U, and T/U base pairs faster than it removes thymine from G/T .
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This record set is specific for:
UNIPROT: Q9V4D8
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Word Map
The expected taxonomic range for this enzyme is: Archaea, Eukaryota
Reaction Schemes
Hydrolyses mismatched double-stranded DNA and polynucleotides, releasing free thymine.
Synonyms
thymine dna glycosylase, thymine-dna glycosylase, mismatch-specific thymine-dna glycosylase, pa-mig, g/t glycosylase, thymine dna-glycosylase, uracil/thymine dna glycosylase, mismatch-specific thymine-dna n-glycosylase, t:g mismatch-specific thymidine-dna glycosylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
thymine DNA-glycosylase
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SYSTEMATIC NAME
IUBMB Comments
thymine-DNA deoxyribohydrolase (thymine-releasing)
Thymine-DNA glycosylase is part of the DNA-repair machinery. Thymine removal is fastest when it is from a G/T mismatch with a 5'-flanking C/G pair. The glycosylase removes uracil from G/U, C/U, and T/U base pairs faster than it removes thymine from G/T [3].
CAS REGISTRY NUMBER
COMMENTARY hide
149565-68-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,N4-ethenocytosine-mismatched double-stranded DNA + H2O
3,N4-ethenocytosine + double-stranded DNA with abasic site
show the reaction diagram
-
-
-
?
5-fluorouracil-mismatched double-stranded DNA + H2O
5-fluorouracil + double-stranded DNA with abasic site
show the reaction diagram
-
-
-
?
5-methylcytosine-mismatched double-stranded DNA + H2O
5-methylcytosine + double-stranded DNA with abasic site
show the reaction diagram
-
-
-
?
thymine-mismatched double-stranded DNA + H2O
thymine + double-stranded DNA with abasic site
show the reaction diagram
G-T mismatch is only a poor substrate for Thd1p
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-
?
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
show the reaction diagram
-
-
-
?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
uracil-mismatched double-stranded DNA + H2O
uracil + double-stranded DNA with abasic site
show the reaction diagram
-
-
-
?
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q9V4D8_DROME
1738
0
191423
TrEMBL
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60000
x * 60000, recombinant enzyme, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 60000, recombinant enzyme, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene Thd1, phylogenetic analysis, functional expression of a His-tagged truncated variant comprising residues 650M-1063N in Escherichia coli strain BL21 (DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hardeland, U.; Bentele, M.; Jiricny, J.; Schaer, P.
The versatile thymine DNA-glycosylase: a comparative characterization of the human, Drosophila and fission yeast orthologs
Nucleic Acids Res.
31
2261-2271
2003
Drosophila melanogaster (Q9V4D8), Drosophila melanogaster, Homo sapiens (Q13569), Homo sapiens
Manually annotated by BRENDA team