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13mer oligonucleotide duplex containing 8-oxoguanine + H2O
8-oxoguanine + oligonucleotide
-
-
-
?
23mer oligonucleotide duplex containing 8-oxoguanine + H2O
8-oxoguanine + oligonucleotide
-
-
-
?
53mer containing 8-oxoguanine + H2O
53mer with an abasic site + 8-oxoguanine
-
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
DNA + 2,6-diamino-4-hydroxy-5-formamidopyrimidine
-
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
DNA + 4,6-diamino-5-formamidopyrimidine
-
-
-
?
DNA containing 8-oxo-7,8-dihydroguanine residues + H2O
DNA + 8-oxo-7,8-dihydroguanine
-
-
-
?
DNA containing 8-oxoguanine opposite A + H2O
8-oxoguanine + DNA
poor substrate, analysis of rate constants of conformational transitions
-
-
?
DNA containing 8-oxoguanine opposite C + H2O
8-oxoguanine + DNA
good substrate, analysis of rate constants of conformational transitions
-
-
?
DNA containing 8-oxoguanine opposite G + H2O
8-oxoguanine + DNA
analysis of rate constants of conformational transitions
-
-
?
DNA containing 8-oxoguanine opposite T + H2O
8-oxoguanine + DNA
analysis of rate constants of conformational transitions
-
-
?
DNA containing 8-oxoguanine residues + H2O
DNA + 8-oxoguanine
best substrate
-
-
?
DNA containing dihydrouracil + H2O
8-oxoguanine + DNA
-
-
-
?
DNA containing formamidopyrimidine-guanine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
-
-
?
DNA containing methylated formamidopyrimidine-guanine residues + H2O
2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
-
-
-
?
13mer oligonucleotide duplex containing 8-oxoguanine + H2O
8-oxoguanine + oligonucleotide
-
-
-
-
?
2,6-diamino-4-hydroxy-5-formamidopyrimidine:Cyt oligodeoxynucleotide + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + Cyt oligodeoxynucleotide
-
-
-
-
?
8-oxo-7,8-dihydroguanine:Cyt oligodeoxynucleotide + H2O
8-oxo-7,8-dihydroguanine + Cyt oligodeoxynucleotide
-
-
-
-
?
DNA containing 2'-deoxy-8-oxonebularine residues + H2O
?
23mer oligonucleotide containing a single site, very poor substrate
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
DNA + 2,6-diamino-4-hydroxy-5-formamidopyrimidine
-
-
-
-
?
DNA containing 2,6-diamino-4-hydroxyformamidopyrimidine residues + H2O
2,6-diamino-4-hydroxyformamidopyrimidine + DNA
-
repair of the major DNA lesions 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxyformamidopyrimidine formed by reactive oxidative species
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
DNA + 4,6-diamino-5-formamidopyrimidine
-
-
-
-
?
DNA containing 5,6-dihydrothymine residues + H2O
?
-
dublex 33mer oligonucleotide, poor substrate
-
-
?
DNA containing 5,6-dihydrouracil + H2O
DNA + 5,6-dihydrouracil
-
-
-
-
?
DNA containing 5-hydroxy-2'-deoxyuridine + H2O
?
-
double-stranded oligonucleotides, N-glycosylase/beta,delta-elimination reaction
-
-
?
DNA containing 5-hydroxycytosine residues + H2O
5-hydroxycytosine + DNA
-
-
-
-
?
DNA containing 5-hydroxycytosine residues + H2O
?
DNA containing 5-hydroxyuracil residues + H2O
5-hydroxyuracil + DNA
-
-
-
-
?
DNA containing 7,8-dihydro-8-oxoguanine residues + H2O
DNA + 7,8-dihydro-8-oxoguanine
-
-
-
-
?
DNA containing 7-deaza-2'-deoxyguanosine + H2O
7-deaza-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing 7-hydro-8-oxoguanine + H2O
7-hydro-8-oxoguanine + DNA
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
DNA containing 7-methyl-7-deazaguanine residues + H2O
7-methyl-7-deazaguanine + DNA
-
-
-
-
?
DNA containing 7-methyl-8-oxo-2'-deoxyguanosine + H2O
7-methyl-8-oxo-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing 8-hydroxyadenine + H2O
DNA + 8-hydroxyguanine
-
-
-
-
?
DNA containing 8-hydroxyadenine residues + H2O
8-hydroxyadenine + DNA
-
poor substrate
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
DNA containing 8-hyroxyguanine + H2O
?
-
-
-
-
?
DNA containing 8-oxo-2'-deoxyguanosine + H2O
8-oxo-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing 8-oxo-2'-deoxyinosine + H2O
?
-
dublex
-
-
?
DNA containing 8-oxo-7,8-dihydroguanine residues + H2O
DNA + 8-hydroxyguanine
-
-
-
-
?
DNA containing 8-oxo-guanine residues + H2O
DNA + 8-oxoguanine
-
-
-
-
?
DNA containing 8-oxo-guanine residues mispaired to adenine + H2O
DNA + 8-oxoguanine
-
-
-
-
?
DNA containing 8-oxo-guanine residues mispaired to guanine + H2O
DNA + 8-oxoguanine
-
-
-
-
?
DNA containing 8-oxo-guanine residues mispaired to thymine + H2O
DNA + 8-oxoguanine
-
-
-
-
?
DNA containing 8-oxoguanine residues + H2O
8-oxoguanine + DNA
DNA containing 8-oxoguanine residues + H2O
DNA + 8-oxoguanine
-
-
-
-
?
DNA containing 8-thio-2'-deoxyguanosine + H2O
8-thio-2'-deoxyguanosine + DNA
-
-
-
-
?
DNA containing dihydrothymine residues + H2O
dihydrothymine + DNA
-
-
-
-
?
DNA containing guanidinohydantoin + H2O
guanidinohydantoin + DNA
-
for DNA duplex length of 30 bp, the excision efficiency in pairs with C, G, or T is similar to 8-oxoguanine. Opposite A, the base removal activity is more efficient than removal of 8-oxoguanine
-
-
?
DNA containing ring-opened N7-methylguanine + H2O
2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine + DNA
-
repairs oxidative DNA damage by efficiently removing formamidopyrimidine lesions and 8-oxoguanine residues from DNA
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
DNA containing spiroiminodihydantoin + H2O
spiroiminodihydantoin + DNA
-
for DNA duplex length of 30 bp, the excision efficiency in pairs with C, G, or T is similar to 8-oxoguanine. Opposite A, the base removal activity is more efficient than removal of 8-oxoguanine
-
-
?
DNA containing tetrahydrofuran residues + H2O
tetrahydrofuran + DNA
-
-
-
-
?
oligonucleotide containing 8-oxo-2'-deoxyguanosine residue + H2O
oligonucleotide + 8-oxo-2'-deoxyguanosine
-
-
-
-
?
oligonucleotide containing abasic site residue + H2O
oligonucleotide + abasic site
-
-
-
-
?
oligonucleotide containing tetrahydrofuran residue + H2O
oligonucleotide + tetrahydrofuran
-
-
-
-
?
additional information
?
-
DNA + H2O
?
-
substrate specificity
-
-
?
DNA + H2O
?
-
with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions
-
-
?
DNA + H2O
?
with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions
-
-
?
DNA + H2O
?
-
role of Lys-155 for substrate binding and product release, AP lyase mechanism
-
-
?
DNA + H2O
?
-
FpG-DNA interactions establish contacts with DNA ligands, which span no more than 9 base-pairs, structural studies of Fpg-DNA complexes
-
-
?
DNA + H2O
?
-
excises oxidized purines from damaged DNA, Schiff base intermediate, enzyme structure, bilobal protein with a wide, positive charged DNA-binding groove and a helix-2-turn-helix motif that participates in DNA binding, damage recognition, catalytic mechanism
-
-
?
DNA + H2O
?
-
removes oxidized purines from oxidatively damaged DNA
-
-
?
DNA + H2O
?
removes oxidized purines from oxidatively damaged DNA
-
-
?
DNA + H2O
?
-
catalytic mechanism involves formation of Schiff base intermediate between DNA containing an oxidized residue and the N-terminal Pro-2 of Fpg, mendatory role of P-2 in 7,8-dihydro-8-oxoguanine-DNA glycosylase and AP lyase activity, but less in 2,6-diamino-4-hydroxy-5-N-methyl-formamidopyrimidine-DNA glycosylase activity
-
-
?
DNA + H2O
?
-
active site is located within the first 73 amino acids of the N-terminus
-
-
?
DNA + H2O
?
-
excises purine bases with ring-opened imidazoles, associated activity that nicks DNA at apurinic/apyrimidinic sites, mechanism of cleavage involves beta elimination
-
-
?
DNA + H2O
?
-
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
646932, 646933, 646935, 646936, 646943, 646944, 646946, 646948, 646950, 646951, 646952, 646954 -
-
?
DNA + H2O
?
enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity
-
-
?
DNA + H2O
?
-
enzyme catalyzes the nicking of both the phosphodiester bonds 3' and 5' of apurinic or apyrimidinic sites in DNA so that the base-free deoxyribose is replaced by a gap limited by 3'-phosphate and 5'-phosphate ends, the 2 nickings are not the result of hydrolytic processes, the enzyme rather catalyzes a beta-elimination reaction immediately followed by a delta-elimination
-
-
?
DNA + H2O
?
-
DNA repair enzyme specific for the removal of purine-derived lesions from DNA damaged by free radicals and other oxidative processes
-
-
?
DNA + H2O
?
-
plays an important role in base excision repair of oxidatively damaged DNA
-
-
?
DNA + H2O
?
-
involved in the repair of oxidized purines generated in the genome by endogenous or exogenous oxidative stress
-
-
?
DNA + H2O
?
-
biological substrates are purine oxidation products
-
-
?
DNA + H2O
?
-
DNA base excision repair enzyme
-
-
?
DNA + H2O
?
DNA base excision repair enzyme
-
-
?
DNA + H2O
?
-
bifunctional base excision repair enzyme: DNA glycosylase/AP lyase
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
-
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
removal with similar specificity as 4,6-diamino-5-formamidopyrimidine and 8-hydroxyguanine
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
FapyGua
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
-
FapyGua
-
-
?
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O
2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA
FapyGua
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
-
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
removal with similar specificity as 2,6-diamino-4-hydroxy-5-formamidopyrimidine and 8-hydroxyguanine
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
FapyAde
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
-
FapyAde
-
-
?
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O
4,6-diamino-5-formamidopyrimidine + DNA
FapyAde
-
-
?
DNA containing 5-hydroxycytosine residues + H2O
?
-
double-stranded oligonucleotides containing 5-hydroxy-2'-deoxycytidine, N-glycosylase/beta,delta-elimination reaction
-
-
?
DNA containing 5-hydroxycytosine residues + H2O
?
-
dublex 33mer oligonucleotide, excision mechanism
-
-
?
DNA containing 7-hydro-8-oxoguanine + H2O
7-hydro-8-oxoguanine + DNA
-
repairs oxidative DNA damage by efficiently removing formamidopyrimidine lesions and 8-oxoguanine residues from DNA
-
-
?
DNA containing 7-hydro-8-oxoguanine + H2O
7-hydro-8-oxoguanine + DNA
-
dublex, primary physiological substrate, DNA repair
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
-
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
24-oligomer, Lys-155 directly interacts with the C8 oxygen of 8-oxopurines involving proton transfer or transient formation of an ion pair between enzyme and substrate, mechanism
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
DNA glycosylase/AP lyase activity
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
the C-8 keto group of 8-oxodeoxyguanine and the carbonyl moiety of formamidopyrimidine enable the enzyme to recognize and bind duplex DNA containing modified bases
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
mechanism involving protonation at O-6 of 8-oxodeoxyguanine
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
dublex 20-oligomer, catalytic mechanism with enzyme-substrate Schiff base intermediate, amino terminal localization of the catalytic site, C-8 keto group of 8-oxodeoxyguanine plays a critical role in binding enzyme
-
-
?
DNA containing 7-hydro-8-oxoguanine residues + H2O
DNA + 7-hydro-8-oxoguanine
-
kinetic mechanism, 3 activities: DNA-glycosylase, beta-elimination/AP-lyase and delta elimination, 12-nucleotide dublex containing 8-oxo-G in the sixth position of one strand, conformational transitions of Fpg protein during the catalytic process
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
-
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
DNA glycosylase/AP lyase activity
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
34mer oligonucleotide containing a single 7,8-dihydro-8-oxoguanine residue
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
dublex oligodeoxynucleotides containing 8-oxo-7,8-dihydro-2-deoxyguanosine positioned opposite dC, dG or dT are cleaved, but not opposite dA or single-stranded DNA, cleaves 3 and 5 to the modified base
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
comparison of repair activities of human OGG1 and Escherichia coli Fpg, enzymes show distinct preferences for the base opposite 8-oxoguanine, mechanism via Schiff base intermediate
treatment of 7,8-dihydro-8-oxoguanine with Fpg results in delta-elimination products
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
mendatory role of P-2 in 7,8-dihydro-8-oxoguanine-DNA glycosylase activity
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
7,8-dihydro-8-oxo-2-deoxyguanosine, natural substrate, substrate recognition, mechanism
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
readily incises dublexes with cytosine, thymine or guanine opposite, but much slower with adenine opposite 7,8-dihydro-8-oxoguanine, 2 activities: DNA-glycosylase and DNA nicking at abasic sites
treatment of 7,8-dihydro-8-oxoguanine with Fpg results in delta-elimination products
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
7,8-dihydro-8-oxoguanine opposite C dublex DNA, formation of a Schiff base intermediate, important role for Lys-57 in the 7,8-dihydro-8-oxoG-DNA glycolase activity in vitro and in vivo
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
7,8-dihydro-8-oxoguanine-DNA
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
7,8-dihydro-8-oxoguanine-DNA
treatment of 7,8-dihydro-8-oxoguanine with Fpg results in delta-elimination products
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
removal with similar specificity as 4,6-diamino-5-formamidopyrimidine and 2,6-diamino-4-hydroxy-5-formamidopyrimidine, important role of Lys-57 in Fpg activity for 8-hydroxyguanine, but lesser significant role for formamidopyrimidines, Pro-2 is critical for substrate recognition and in catalysis of its excision
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
54 nt DNA oligomer, CoFpg and ZnFpg are equally active at cleaving the DNA at the site of the oxidized guanine
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
natural substrate: 7,8-dihydro-8-oxo-dG, DNA base excision repair
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
dublex, primary physiological substrate is 7,8-dihydro-8-oxoguanine-DNA, DNA repair
-
-
?
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
-
repair of the major DNA lesions 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxyformamidopyrimidine formed by reactive oxidative species
-
-
?
DNA containing 8-oxoguanine residues + H2O
8-oxoguanine + DNA
-
-
-
-
?
DNA containing 8-oxoguanine residues + H2O
8-oxoguanine + DNA
-
8-oxoguanine residues opposite cytosine
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
-
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
enzyme excises the secondary alkylation product of 7-methylguanine Fapy
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
double-stranded DNA is preferred to single-stranded DNA
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
dublex oligodeoxynucleotides containing Me-Fapy
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
comparison of repair activities of human OGG1 and Escherichia coli Fpg, almost no paired base-dependent repair, effect of sequence context on repair efficiency, mechanism via Schiff base intermediate
treatment of me-Fapy with Fpg results in delta-elimination products
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
23-oligomer
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
less mendatory role of P-2 in 2,6-diamino-4-hydroxy-5-N-methyl-formamidopyrimidine-DNA glycosylase activity
-
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
the C-8 keto group of 8-oxodeoxyguanine and the carbonyl moiety of formamidopyrimidine enable the enzyme to recognize and bind duplex DNA containing modified bases, mechanism
-
?
DNA containing ring-opened N7-methylguanine residues + H2O
2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA
-
amino terminal localization of the catalytic site
-
?
additional information
?
-
-
not: carbocyclic substrate analog of 8-oxo-7,8-dihydro-2-deoxyguanosine
-
-
?
additional information
?
-
-
no cleavage of 3-methyladenine, uracil, intact 7-methylguanine from DNA
-
-
?
additional information
?
-
-
not: dublex DNA containing a single tetrahydrofuran residue
-
-
?
additional information
?
-
-
human OGG1 and Escherichia coli Fpg are structurally unrelated enzymes with different catalytic residues, OGG1: Lys-249 and Asp-268 in the hairpin-helix-hairpin-GDP motif are involved in the glycosylase/AP lyase activity, Fpg: uses a proline residue in the N-terminal region for catalysis
-
-
?
additional information
?
-
-
not: DNA containing 8-oxo-7,8-dihydro-2-deoxyadenosine, single-stranded DNA, dublex DNA containing synthetic abasic sites, mismatches containing dG, unmodified DNA
-
-
?
additional information
?
-
-
base excision repair initiated by the enzyme is less effective in the first two days of growth and more effective later in stationary phase
-
-
?
additional information
?
-
-
enzyme mediates repair of lesions containing hydantoins in vivo
-
-
?
additional information
?
-
-
cross-linking of active center with a series of reactive oligonucleotide duplexes containing both a single 8-oxoguanine residue and an O-ethyl-substituted diphosphate internucleotide group results in identification of seven phosphate groups on both strands of the DNA duplex specifically interacting with nucleophilic amino acids of the enzyme. L56 of enzyme cross-links to the phosphate located 3' to the 8-oxoguanine residue
-
-
?
additional information
?
-
-
defective repair of 5-hydroxy-2-deoxycytidine in Cockayne syndrome cells is complementated by Escherichia coli formamidopyrimidine DNA glycosylase and endonuclease III
-
-
?
additional information
?
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formamidopyrimidine-DNA N-glycosylase operates in the base excision repair pathway in bacteria by removing oxidized guanine bases from DNA and can also cleave the nascent or preformed abasic DNA by beta,delta-elimination. The cleaved product formation is initially reversible
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additional information
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FPG excises oxidatively damaged purines in the base excision repair pathway, overview
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additional information
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-
formamidopyrimidine-DNA N-glycosylase removes oxidized guanine bases from DNA. The cleaved product formation is initially reversible, it is followed by conformational changes in the enzyme and DNA molecules that represent the postchemical irreversible rate-limiting steps. The overall rate-limiting step of the enzymatic reaction seems to be the release of Fpg from its adduct with the 4-oxo-2-pentenal remnant of the deoxyribose moiety formed as a result of DNA strand cleavage by beta,delta-elmination, the initial chemical steps are fast and reversible. Catalytic mechanism, overview
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additional information
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-
FPG excises oxidatively damaged purines in the base excision repair pathway, it acts on DNA containing 5,6-dihydrouracil, 8-oxo-7,8-dihydroguanine, or on apurinic/apyrimidinic DNA base pairs, analysis of conformational dynamics of Fpg protein and DNA substrates, rate constants of conformational transitions, and intrinsic mechanism of recognition and excision of damaged bases in DNA, overview
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additional information
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formamidopyrimidine DNA glycosylase is specific for oxidized purines
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additional information
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the enzyme is more active towards oxidized purines than oxidized pyrimidines and has little to no activity toward DNA containing thymine glycols and osmium-tetroxide-treated DNA
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additional information
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analysis of acidity and proton affinity for a range of substrates such as 8-oxoguanine, 8-oxoadenine, 8-oxoinosine, 8-oxonebularine, formamidopyrimidine-guanine, 5-hydroxyuracil, and 5,6-dihydrothymine. The most favorable mechanism involves preprotonation of the O4', which results in the opening of the ribose ring
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0.0000039 - 0.0000088
13mer oligonucleotide duplex containing 8-oxoguanine
-
0.0000063 - 0.000018
23mer oligonucleotide duplex containing 8-oxoguanine
-
0.0000181 - 0.0000466
13mer oligonucleotide duplex containing 8-oxoguanine
-
0.00178 - 0.00487
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues
-
0.00078 - 0.00129
DNA containing 4,6-diamino-5-formamidopyrimidine residues
-
0.00045
DNA containing 5,6-dihydrothymine residues
-
pH 7.5, 37°C
-
0.00061 - 0.00957
DNA containing 5,6-dihydrouracil
0.0047
DNA containing 5-hydroxycytosine residues
-
pH 7.5, 37°C
-
0.000083
DNA containing 7-deaza-2'-deoxyguanosine
-
-
0.0000069 - 0.002
DNA containing 7-hydro-8-oxoguanine residues
-
0.000053
DNA containing 7-methyl-8-oxo-2'-deoxyguanosine
-
-
0.000004 - 0.00311
DNA containing 8-hydroxyguanine residues
-
0.000012
DNA containing 8-oxo-2'-deoxyguanosine
-
-
0.001 - 0.44
DNA containing 8-oxo-guanine residues
-
0.022 - 0.023
DNA containing 8-oxo-guanine residues mispaired to adenine
-
0.0001 - 0.0051
DNA containing 8-oxo-guanine residues mispaired to guanine
-
0.00019 - 0.0042
DNA containing 8-oxo-guanine residues mispaired to thymine
-
0.000225
DNA containing 8-thio-2'-deoxyguanosine
-
-
0.000009 - 0.000041
DNA containing ring-opened N7-methylguanine residues
-
additional information
additional information
-
0.0000039
13mer oligonucleotide duplex containing 8-oxoguanine
mutant R244E, pH 7.5, 37°C
-
0.0000047
13mer oligonucleotide duplex containing 8-oxoguanine
mutant S208A, pH 7.5, 37°C
-
0.000006
13mer oligonucleotide duplex containing 8-oxoguanine
mutant Q234R, pH 7.5, 37°C
-
0.0000081
13mer oligonucleotide duplex containing 8-oxoguanine
wild-type, pH 7.5, 37°C
-
0.0000088
13mer oligonucleotide duplex containing 8-oxoguanine
mutant Q234R/R244E, pH 7.5, 37°C
-
0.0000063
23mer oligonucleotide duplex containing 8-oxoguanine
mutant R258Q, pH 7.5, 30°C
-
0.0000081
23mer oligonucleotide duplex containing 8-oxoguanine
wild-type, pH 7.5, 30°C
-
0.0000081
23mer oligonucleotide duplex containing 8-oxoguanine
mutant N168Q, pH 7.5, 30°C
-
0.000018
23mer oligonucleotide duplex containing 8-oxoguanine
mutant R108K, pH 7.5, 30°C
-
0.0000181
13mer oligonucleotide duplex containing 8-oxoguanine
-
15°C
-
0.0000222
13mer oligonucleotide duplex containing 8-oxoguanine
-
17.5°C
-
0.0000296
13mer oligonucleotide duplex containing 8-oxoguanine
-
20°C
-
0.0000369
13mer oligonucleotide duplex containing 8-oxoguanine
-
22.5°C
-
0.0000466
13mer oligonucleotide duplex containing 8-oxoguanine
-
25°C
-
0.0000009
DNA
pH 7.5, 20°C, 24-oligomer DNA containing apurinic/apyrimidinic sites, lyase activity, K155A mutant Fpg
0.000019
DNA
pH 7.5, 20°C, 24-oligomer DNA containing AP sites, lyase activity, wild-type Fpg
0.0046
DNA
-
pH 7.5, 25°C, DNA containing an apurinic/apyrimidinic site
0.00178
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues
-
pH 7.4, 37°C, K57R mutant Fpg, from DNA gamma-irradiated under NO2
-
0.00187
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues
-
pH 7.4, 37°C, K57G mutant Fpg, from DNA gamma-irradiated under NO2
-
0.00343 - 0.00376
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues
-
pH 7.4, 37°C, wild-type Fpg
-
0.00422
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues
-
pH 7.4, 37°C, K57G mutant Fpg, from DNA treated with H2O2/Fe(III)-EDTA/asc
-
0.00487
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues
-
pH 7.4, 37°C, K57R mutant Fpg, from DNA treated with H2O2/Fe(III)-EDTA/asc
-
0.00078 - 0.00084
DNA containing 4,6-diamino-5-formamidopyrimidine residues
-
pH 7.4, 37°C, K57G mutant Fpg
-
0.00079 - 0.0009
DNA containing 4,6-diamino-5-formamidopyrimidine residues
-
pH 7.4, 37°C, K57R mutant Fpg
-
0.00107 - 0.00129
DNA containing 4,6-diamino-5-formamidopyrimidine residues
-
pH 7.4, 37°C, wild-type Fpg
-
0.00061
DNA containing 5,6-dihydrouracil
-
mutant K217A
0.00068
DNA containing 5,6-dihydrouracil
-
wild-type
0.0007
DNA containing 5,6-dihydrouracil
-
mutant H89A
0.0007
DNA containing 5,6-dihydrouracil
-
mutant R108A
0.00465
DNA containing 5,6-dihydrouracil
-
mutant R109A
0.00957
DNA containing 5,6-dihydrouracil
-
mutant H89A/R109A
0.0000069
DNA containing 7-hydro-8-oxoguanine residues
pH 7.5, 37°C, 24-oligomer, wild-type Fpg
-
0.0000075
DNA containing 7-hydro-8-oxoguanine residues
pH 7.5, 37°C, 24-oligomer, K155A mutant Fpg
-
0.002
DNA containing 7-hydro-8-oxoguanine residues
-
pH 7.5, 25°C
-
0.000004
DNA containing 8-hydroxyguanine residues
-
pH 7.8, 37°C, 34-oligomer, wild-type and K57R mutant Fpg
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0.000008
DNA containing 8-hydroxyguanine residues
-
pH 7.8, 37°C, 34-oligomer, K57G mutant Fpg
-
0.000008
DNA containing 8-hydroxyguanine residues
-
pH 7.5, 20°C, dublex DNA containing a single 8-oxoguanine residue positioned opposite dC
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0.000013
DNA containing 8-hydroxyguanine residues
-
pH 7.5, 37°C
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0.00093 - 0.00155
DNA containing 8-hydroxyguanine residues
-
pH 7.4, 37°C, K57R mutant Fpg
-
0.00109
DNA containing 8-hydroxyguanine residues
-
pH 7.4, 37°C, wild-type Fpg, from DNA treated with H2O2/Fe(III)-EDTA/asc
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0.00126
DNA containing 8-hydroxyguanine residues
-
pH 7.4, 37°C, K57G mutant Fpg
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0.00311
DNA containing 8-hydroxyguanine residues
-
pH 7.4, 37°C, wild-type Fpg, from DNA gamma-irradiated under NO2
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0.001
DNA containing 8-oxo-guanine residues
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wild-type
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0.0015
DNA containing 8-oxo-guanine residues
-
mutant R108A
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0.0041
DNA containing 8-oxo-guanine residues
-
mutant H89A
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0.0041
DNA containing 8-oxo-guanine residues
-
mutant K217T
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0.227
DNA containing 8-oxo-guanine residues
-
mutant R109A
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0.44
DNA containing 8-oxo-guanine residues
-
mutant H89A/R109A
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0.022
DNA containing 8-oxo-guanine residues mispaired to adenine
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wild-type
-
0.023
DNA containing 8-oxo-guanine residues mispaired to adenine
-
mutant R108A
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0.0001
DNA containing 8-oxo-guanine residues mispaired to guanine
-
wild-type
-
0.0051
DNA containing 8-oxo-guanine residues mispaired to guanine
-
mutant R108A
-
0.00019
DNA containing 8-oxo-guanine residues mispaired to thymine
-
wild-type
-
0.0042
DNA containing 8-oxo-guanine residues mispaired to thymine
-
mutant R108A
-
0.000009 - 0.000011
DNA containing ring-opened N7-methylguanine residues
-
pH 7.8, 37°C, wild-type, K57G and K57R mutant Fpg
-
0.000023
DNA containing ring-opened N7-methylguanine residues
pH 7.5, 37°C, 23-oligomer, wild-type Fpg
-
0.000029
DNA containing ring-opened N7-methylguanine residues
pH 7.5, 37°C, 23-oligomer, K155A mutant Fpg
-
0.000038
DNA containing ring-opened N7-methylguanine residues
-
pH 7.5, 37°C
-
0.000041
DNA containing ring-opened N7-methylguanine residues
-
pH 7.5, 20°C, dublex DNA containing a single Me-Fapy residue positioned opposite dC
-
additional information
additional information
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thermodynamic characterization of Fpg binding to lesion-containing 13mer DNA dublexes, binding affinity
-
additional information
additional information
-
Km for DNA substrate is affected by the base opposite the lesion
-
additional information
additional information
-
kinetic parameters of duplex oligonucleotides and mismatched duplexes
-
additional information
additional information
-
kinetics, detailed overview
-
additional information
additional information
-
transient kinetics of Fpg
-
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0.0065 - 0.0283
13mer oligonucleotide duplex containing 8-oxoguanine
-
0.07 - 1.7
23mer oligonucleotide duplex containing 8-oxoguanine
-
0.009 - 0.0273
13mer oligonucleotide duplex containing 8-oxoguanine
-
0.004 - 0.015
DNA containing 5,6-dihydrouracil
0.053
DNA containing 7-deaza-2'-deoxyguanosine
-
-
0.000383 - 0.025
DNA containing 7-hydro-8-oxoguanine residues
-
0.00051
DNA containing 7-methyl-8-oxo-2'-deoxyguanosine
-
-
0.000167 - 0.03
DNA containing 8-hydroxyguanine residues
-
0.00167
DNA containing 8-hyroxyguanine
-
pH 7.8, 37°C, 34-oligomer, K57R mutant Fpg
-
0.066
DNA containing 8-oxo-2'-deoxyguanosine
-
-
0.0003 - 0.0067
DNA containing 8-oxo-guanine residues
-
0.000075 - 0.00011
DNA containing 8-oxo-guanine residues mispaired to adenine
-
0.00038 - 0.0035
DNA containing 8-oxo-guanine residues mispaired to guanine
-
0.0003 - 0.0047
DNA containing 8-oxo-guanine residues mispaired to thymine
-
0.00045
DNA containing 8-thio-2'-deoxyguanosine
-
-
0.0012 - 0.085
DNA containing ring-opened N7-methylguanine residues
-
additional information
additional information
-
turnover of duplex oligonucleotides and mismatched duplexes
-
0.0065
13mer oligonucleotide duplex containing 8-oxoguanine
mutant R244E, pH 7.5, 37°C
-
0.0133
13mer oligonucleotide duplex containing 8-oxoguanine
mutant S208A, pH 7.5, 37°C
-
0.0148
13mer oligonucleotide duplex containing 8-oxoguanine
mutant Q234R/R244E, pH 7.5, 37°C
-
0.0153
13mer oligonucleotide duplex containing 8-oxoguanine
mutant Q234R, pH 7.5, 37°C
-
0.0283
13mer oligonucleotide duplex containing 8-oxoguanine
wild-type, pH 7.5, 37°C
-
0.07
23mer oligonucleotide duplex containing 8-oxoguanine
mutant R108K, pH 7.5, 30°C
-
0.08
23mer oligonucleotide duplex containing 8-oxoguanine
mutant R258Q, pH 7.5, 30°C
-
0.16
23mer oligonucleotide duplex containing 8-oxoguanine
mutant N168Q, pH 7.5, 30°C
-
1.7
23mer oligonucleotide duplex containing 8-oxoguanine
wild-type, pH 7.5, 30°C
-
0.009
13mer oligonucleotide duplex containing 8-oxoguanine
-
15°C
-
0.0106
13mer oligonucleotide duplex containing 8-oxoguanine
-
17.5°C
-
0.0148
13mer oligonucleotide duplex containing 8-oxoguanine
-
20°C
-
0.0202
13mer oligonucleotide duplex containing 8-oxoguanine
-
22.5°C
-
0.0273
13mer oligonucleotide duplex containing 8-oxoguanine
-
25°C
-
0.05
DNA
-
pH 7.5, 25°C, DNA containing an apurininc/apyrimidinic site
0.0568
DNA
pH 7.5, 20°C, 24-oligomer DNA containing AP sites, lyase activity, wild-type Fpg
0.0883
DNA
pH 7.5, 20°C, 24-oligomer DNA containing AP sites, lyase activity, K155A mutant Fpg
0.004
DNA containing 5,6-dihydrouracil
-
mutant H89A/R109A
0.0053
DNA containing 5,6-dihydrouracil
-
mutant R108A
0.0053
DNA containing 5,6-dihydrouracil
-
mutant R109A
0.0093
DNA containing 5,6-dihydrouracil
-
mutant K217A
0.011
DNA containing 5,6-dihydrouracil
-
wild-type
0.015
DNA containing 5,6-dihydrouracil
-
mutant H89A
0.000383
DNA containing 7-hydro-8-oxoguanine residues
pH 7.5, 37°C, 24-oligomer, K155A mutant Fpg
-
0.0185
DNA containing 7-hydro-8-oxoguanine residues
pH 7.5, 37°C, 24-oligomer, wild-type Fpg
-
0.025
DNA containing 7-hydro-8-oxoguanine residues
-
pH 7.5, 25°C
-
0.000167
DNA containing 8-hydroxyguanine residues
-
pH 7.8, 37°C, 34-oligomer, K57G mutant Fpg
-
0.00717
DNA containing 8-hydroxyguanine residues
-
pH 7.8, 37°C, 34-oligomer, wild-type Fpg
-
0.03
DNA containing 8-hydroxyguanine residues
-
pH 7.5, 37°C
-
0.0003
DNA containing 8-oxo-guanine residues
-
mutant R108A
-
0.0035
DNA containing 8-oxo-guanine residues
-
mutant H89A/R109A
-
0.004
DNA containing 8-oxo-guanine residues
-
mutant K217T
-
0.005
DNA containing 8-oxo-guanine residues
-
wild-type
-
0.0059
DNA containing 8-oxo-guanine residues
-
mutant R109A
-
0.0067
DNA containing 8-oxo-guanine residues
-
mutant H89A
-
0.000075
DNA containing 8-oxo-guanine residues mispaired to adenine
-
mutant R108A
-
0.00011
DNA containing 8-oxo-guanine residues mispaired to adenine
-
wild-type
-
0.00038
DNA containing 8-oxo-guanine residues mispaired to guanine
-
mutant R108A
-
0.0035
DNA containing 8-oxo-guanine residues mispaired to guanine
-
wild-type
-
0.0003
DNA containing 8-oxo-guanine residues mispaired to thymine
-
mutant R108A
-
0.0047
DNA containing 8-oxo-guanine residues mispaired to thymine
-
wild-type
-
0.0012
DNA containing ring-opened N7-methylguanine residues
pH 7.5, 37°C, 23-oligomer, K155A mutant Fpg
-
0.002
DNA containing ring-opened N7-methylguanine residues
-
pH 7.8, 37°C, K57G and K57R mutant Fpg
-
0.00383
DNA containing ring-opened N7-methylguanine residues
pH 7.5, 37°C, 23-oligomer, wild-type Fpg
-
0.00833
DNA containing ring-opened N7-methylguanine residues
-
pH 7.8, 37°C, wild-type Fpg
-
0.085
DNA containing ring-opened N7-methylguanine residues
-
pH 7.5, 37°C
-
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E173Q
no enzymic activity, E173 may play a crucial role in forming the active site pocket
E2Q
no enzymic activity, interactions with G167 and Y170 are interupted
H71A
severely compromised in turnover of oligonucleotides with 8-oxoguanosine opposie cytosine, but show turnover rates comparable to wild-type on abasic-site containing DNA
N168D
0.2% of wild-type activity
N168Q
9.5% of wild-type activity
Q234R
mutant retains activity
Q234R/R244E
mutant retains activity
R108K
1.9% of wild-type activity
R108L
0.3% of wild-type activity
R108Q
0.3% of wild-type activity
R244E
mutant retains activity
R258A
0.4% of wild-type activity
R258K
0.3% of wild-type activity
R258Q
6.2% of wild-type activity
R54E/E131R
loss of activity
S208A
mutation has no effect, in both wild-type and S208 A residue Tyr170 quickly reorients to form an alternative set of hydrogen bonds
Y170F
mutation decreases Fpg binding but does not fully inactivate the protein
E3Q
-
inactive. Mutant binds DNA duplexes containing spiroiminodihydantoin or guanidinohydantoin about 1000fold more tightly over corresponding duplexes containing 8-oxoguanine
F110A
-
the mutation affects the enzyme activity, especially in the case of oxoG/C substrate, in the second and third reaction steps
F110W
-
the mutation affects the enzyme activity, especially in the case of oxoG/C substrate, in the second and third reaction steps
F111A
-
the mutant displays a significant increase in the average diffusion constant compared to the wild-type protein with no enzymatic activity on DNA containing 8-oxoguanine residues opposite cytosine. The mutant has little or no ability to form a Schiff base with 8-oxoguanine residues opposite cytosine or 5,6-dihydrouracil opposite guanine compared to wild type enzyme
H89A
-
selective diminition of the rate of excision of 8-oxoguanine
H89A/R109A
-
about 10fold increase in KM-value
K217T
-
selective reduction of the ability to excise 8-oxoguanine from DNA
K57A
-
mutant with about 15% of wild-type activity in both N-glycosylase and AP lyase activity
P2T
-
mutant with 10% of wild-type 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine DNA glycosylase activity and barely detectable 7,8-dihydro-8-oxoguanine-DNA glycosylase activity, no cleavage of DNA containing AP sites
R108A
-
R108 is a major determinant of opposite-base specificity
R109A
-
binding of enzyme to damaged DNA is almost abolished
K155A
-
effect of mutation on specificity, mutant with very low activity, kinetic study
K155A
mutant with 50fold decreased activity with 7-hydro-8-oxoguanine-DNA as substrate, only 3-4fold decreased activity with 7-methylformamidopyrimidine-DNA, increased AP lyase activity
K155A
-
mutant with reduced 8-oxoguanine-DNA but unchanged Fapy-DNA glycosylase activity
K155A
-
mutant enzyme with decreased N-glycosylase and increased AP lyase activity, it dissociates prematurely from the covalent enzyme-DNA complex leading to a higher turnover number for DNA containing an AP site
K57G
-
study of the effect of the mutation on the structure dynamics, mutant with decreased 8-hydroxyguanine-DNA glycosylase activity
K57G
-
mutant has dramatically reduced 7,8-dihydro-8-oxoguanine-DNA glycosylase activity and is poorly effective in formation of Schiff base complex with 8-oxoG/C, little effect on 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine-DNA glycosylase activity, no effect on DNA nicking activity at abasic sites
K57G
-
effect of mutation on specificity, mutant removes FapyAde and FapyGua with reduced activity compared to wild-type Fpg, kinetic study
K57R
-
effect of mutation on specificity, mutant removes FapyAde and FapyGua with reduced activity compared to wild-type Fpg, kinetic study
K57R
-
slight effect of mutation on 7,8-dihydro-8-oxoguanine-DNA glycosylase activity, no effect on 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine-DNA glycosylase activity and on DNA nicking activity at abasic sites
P2E
-
study of the effect of the mutation on the structure dynamics, mutation causes complete loss of DNA glycosylase/beta-lyase activity and induces a conformational change leading to a more rigid globular structure than wild-type, K57G and P2G Fpg
P2E
-
effect of mutation on specificity, inactive mutant, kinetic study
P2E
-
completely inactive mutant: no 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine DNA/7,8-dihydro-8-oxoguanine-DNA glycosylase activity or cleavage of DNA containing AP sites
P2G
-
effect of mutation on specificity, mutant with very low activity, kinetic study
P2G
-
study of the effect of the mutation on the structure dynamics, mutant with complete loss of beta-lyase and partial loss of DNA glycosylase activity
P2G
-
mutant with 10% of wild-type 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine DNA glycosylase activity and barely detectable 7,8-dihydro-8-oxoguanine-DNA glycosylase activity, no cleavage of DNA containing AP sites
additional information
-
mutant Fpg protein with NH2-terminal modifications
additional information
-
expression in Chinese hamster ovary cells results in decrease of the levels of oxypurine clustered damages while those of oxypyrimidine clusters and abasic clusters are unchanged. Growth rates of cells are increased and the level of spontaneous background mutants in the hypoxanthine guanine phosphoribosyl transferase gene is decreased
additional information
-
expression of enzyme in human cells from patients belonging to Cockayne syndrome complementation groups A and B completely corrects the repair deficiency in both CS-A and CS-B cells. The sensitivity of CS-B cells to elevated concentrations of potassium bromate is not compensated by expression of enzyme
additional information
-
expression of enzyme-green fluorescent protein fusion protein in human bladder cells. Cells expressing the fusion protein repair 8-oxoguanine and abasic sites at accelerated rates and are resistant to the oxidizing carcinogen potassium bromate
additional information
-
monitoring of spontaneous revertants in a background in which a T/G replacement inactivates the lacZ gene, in strains possessing and lacking Fpg activity. In strains without enzymic activity grown on glucose medium, the proportion of revertants increases over a 5-day period. In contrast, in strains with enzymic activity, revertants appear primarily during the first 2-3 days after plating, few new revertants appear in the following days
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Boiteux, S.; O'Connor, T.R.; Laval, J.
Formamidopyrimidine-DNA glycosylase of Escherichia coli: cloning and sequencing of the fpg structural gene and overproduction of the protein
EMBO J.
6
3177-3183
1987
Escherichia coli, Escherichia coli HB1100
brenda
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Escherichia coli
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Escherichia coli
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Escherichia coli, Escherichia coli BH20
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Escherichia coli (P50465), Escherichia coli
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Escherichia coli
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Escherichia coli, Escherichia coli BH20
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Escherichia coli, Escherichia coli B834 (DE3)
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Escherichia coli
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Escherichia coli (P05523), Escherichia coli
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Buchko, G.W.; McAteer, K.; Wallace, S.S.; Kennedy, M.A.
Solution-state NMR investigation of DNA binding interactions in Escherichia coli formamidopyrimidine-DNA glycosylase (Fpg): a dynamic description of the DNA/protein interface
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2005
Escherichia coli
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Zaika, E.I.; Perlow, R.A.; Matz, E.; Broyde, S.; Gilboa, R.; Grollman, A.P.; Zharkov, D.O.
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Escherichia coli
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Escherichia coli
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Escherichia coli
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Harbut, M.B.; Meador, M.; Dodson, M.L.; Lloyd, R.S.
Modulation of the turnover of formamidopyrimidine DNA glycosylase
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Escherichia coli (P05523), Escherichia coli
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Kuznetsov, N.A.; Koval, V.V.; Zharkov, D.O.; Vorobjev, Y.N.; Nevinsky, G.A.; Douglas, K.T.; Fedorova, O.S.
Pre-steady-state kinetic study of substrate specificity of Escherichia coli formamidopyrimidine--DNA glycosylase
Biochemistry
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2007
Escherichia coli (P05523), Escherichia coli
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Krishnamurthy, N.; Muller, J.G.; Burrows, C.J.; David, S.S.
Unusual structural features of hydantoin lesions translate into efficient recognition by Escherichia coli Fpg
Biochemistry
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2007
Escherichia coli
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Paul, S.; Gros, L.; Laval, J.; Sutherland, B.M.
Expression of the E. coli fpg protein in CHO cells lowers endogenous oxypurine clustered damage levels and decreases accumulation of endogenous Hprt mutations
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Escherichia coli
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Escherichia coli
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Accelerated repair and reduced mutagenicity of oxidative DNA damage in human bladder cells expressing the E. coli FPG protein
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Escherichia coli
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Hamm, M.L.; Gill, T.J.; Nicolson, S.C.; Summers, M.R.
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Escherichia coli, Homo sapiens
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Escherichia coli, Homo sapiens
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Smith, C.C.; ODonovan, M.R.; Martin, E.A.
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Escherichia coli, Homo sapiens
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Minetti, C.A.; Remeta, D.P.; Breslauer, K.J.
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Escherichia coli
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Kuznetsov, N.A.; Zharkov, D.O.; Koval, V.V.; Buckle, M.; Fedorova, O.S.
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Escherichia coli
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Foresta, M.; Ropolo, M.; Degan, P.; Pettinati, I.; Kow, Y.W.; Damonte, G.; Poggi, A.; Frosina, G.
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Escherichia coli
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Koval, V.V.; Kuznetsov, N.A.; Ishchenko, A.A.; Saparbaev, M.K.; Fedorova, O.S.
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Escherichia coli
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Schalow, B.J.; Courcelle, C.T.; Courcelle, J.
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Escherichia coli, Escherichia coli SR108
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Dunn, A.R.; Kad, N.M.; Nelson, S.R.; Warshaw, D.M.; Wallace, S.S.
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Escherichia coli
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Reddy, P.T.; Jaruga, P.; Nelson, B.C.; Lowenthal, M.; Dizdaroglu, M.
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Escherichia coli
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Rana, J.; Huang, H.
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Escherichia coli
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Kain, J.; Karlsson, H.L.; Moeller, L.
DNA damage induced by micro- and nanoparticles - interaction with FPG influences the detection of DNA oxidation in the comet assay
Mutagenesis
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2012
Escherichia coli
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Kuznetsov, N.A.; Vorobjev, Y.N.; Krasnoperov, L.N.; Fedorova, O.S.
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Escherichia coli
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Foresta, M.; Izzotti, A.; La Maestra, S.; Micale, R.; Poggi, A.; Vecchio, D.; Frosina, G.
Accelerated repair and reduced mutagenicity of DNA damage induced by cigarette smoke in human bronchial cells transfected with E.coli formamidopyrimidine DNA glycosylase
PLoS ONE
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Escherichia coli
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Prakash, A.; Doublie, S.; Wallace, S.S.
The Fpg/Nei family of DNA glycosylases: substrates, structures, and search for damage
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Escherichia coli (P05523), Geobacillus stearothermophilus (P84131), Lactococcus lactis (P42371), Thermus thermophilus (O50606), Thermus thermophilus DSM 579 (O50606)
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Le Meur, R.; Culard, F.; Nadan, V.; Goffinont, S.; Coste, F.; Guerin, M.; Loth, K.; Landon, C.; Castaing, B.
The nucleoid-associated protein HU enhances 8-oxoguanine base excision by the formamidopyrimidine-DNA glycosylase
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Escherichia coli (P05523)
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Kiruba, G.S.; Xu, J.; Zelikson, V.; Lee, J.K.
Gas-phase studies of formamidopyrimidine glycosylase (Fpg) substrates
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2016
Escherichia coli
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Endutkin, A.V.; Koptelov, S.S.; Popov, A.V.; Torgasheva, N.A.; Lomzov, A.A.; Tsygankova, A.R.; Skiba, T.V.; Afonnikov, D.A.; Zharkov, D.O.
Residue coevolution reveals functionally important intramolecular interactions in formamidopyrimidine-DNA glycosylase
DNA Repair
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2018
Escherichia coli (P05523), Escherichia coli
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Li, H.; Endutkin, A.V.; Bergonzo, C.; Campbell, A.J.; de los Santos, C.; Grollman, A.; Zharkov, D.O.; Simmerling, C.
A dynamic checkpoint in oxidative lesion discrimination by formamidopyrimidine-DNA glycosylase
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Escherichia coli (P05523), Geobacillus stearothermophilus (P84131)
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