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Information on EC 3.2.2.23 - DNA-formamidopyrimidine glycosylase and Organism(s) Arabidopsis thaliana and UniProt Accession O80358

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EC Tree
     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.23 DNA-formamidopyrimidine glycosylase
IUBMB Comments
May play a significant role in processes leading to recovery from mutagenesis and/or cell death by alkylating agents. Also involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: O80358
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
neil1, neil3, fpg protein, formamidopyrimidine-dna glycosylase, formamidopyrimidine dna glycosylase, 8-oxoguanine-dna glycosylase, formamidopyrimidine glycosylase, fapy-dna glycosylase, fpg-1, fpg-l, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
formamidopyrimidine glycosylase
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formamidopyrimidine-DNA glycosylase
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2,6-diamino-4-hydroxy-5(N-methyl)formamidopyrimidine-DNA glycosylase
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2,6-diamino-4-hydroxy-5N-formamidopyrimidine-DNA glycosylase
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2,6-diamino-4-hydroxy-5N-methyl-formamidopyrimidine-DNA glycosylase
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8-hydroxyguanine endonuclease
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8-oxoguanine DNA glycosylase
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deoxyribonucleate glycosidase
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DNA glycohydrolase (releasing 2,6-diamino-4-hydroxy-5-(N-methyl)-formamidopyrimidine)
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Fapy-DNA glycosylase
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formamidopyrimidine DNA glycosylase
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formamidopyrimidine-DNA glycosyl hydrolase
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formamidopyrimidine-DNA glycosylase
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Fpg protein
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glycosidase, deoxyribonucleate formamidopyrimidine
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MutM
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-
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additional information
Fpg is one of several DNA glycosylases in Arabidopsis thaliana
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of N-glycosyl bond
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SYSTEMATIC NAME
IUBMB Comments
DNA glycohydrolase [2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimide releasing]
May play a significant role in processes leading to recovery from mutagenesis and/or cell death by alkylating agents. Also involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA.
CAS REGISTRY NUMBER
COMMENTARY hide
78783-53-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DNA + H2O
?
show the reaction diagram
DNA containing 8-hydroxyguanine residues + H2O
8-hydroxyguanine + DNA
show the reaction diagram
AtFPG-1, but not AtFPG-2, cleaves double-stranded oligonucleotides containing 8-oxoguanine
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-
?
DNA containing tetrahydrofuran residues + H2O
tetrahydrofuran + DNA
show the reaction diagram
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-
-
?
depurinated supercoiled plasmid DNA + H2O
?
show the reaction diagram
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the degree of supercoiling of assay plasmid DNA does not affect enzyme activity
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-
?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DNA + H2O
?
show the reaction diagram
catalyzes the initial steps in the repair of DNA containing oxidized purines
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
assay at room temperature
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
FPG_ARATH
390
0
43194
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32400
x * 32400, AtFPG-2, calculated from the amino acid sequence
34000
x * 34000, AtFPG-2, SDS-PAGE
44800
x * 44800, AtFPG-1, calculated from the amino acid sequence
46000
x * 46000, AtFPG-1, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 20% (v/v) PEG 2000 MME, 100 mM KBr, and 100 mM sodium acetate pH 5.0
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HiTrap nickel sulfate-charged column chromatography and HiTrap SP column chromatography
recombinant AtFPG-1, expressed in Escherichia coli BL21(DE3)
recombinant AtFPG-2, expressed in Escherichia coli BL21(DE3)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta (DE3) pLysS cells
gene fpg, expression in Escherichia coli, expression increases the frequency of spontaneous reversions in the presence of plant FPG variant, quantitative overview
isolation of 2 cDNA clones: alternative splicing products AtFPG-1 and -2 of a single gene with different C-terminal sequences, amino acid sequences, expression of both proteins in Escherichia coli BL21(DE3)
gene fpg, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gao, M.J.; Murphy, T.M.
Alternative forms of formamidopyrimidine-DNA glycosylase from Arabidopsis thaliana
Photochem. Photobiol.
73
128-134
2001
Arabidopsis thaliana (O80358), Arabidopsis thaliana
Manually annotated by BRENDA team
Murphy, T.M.; George, A.
A comparison of two DNA base excision repair glycosylases from Arabidopsis thaliana
Biochem. Biophys. Res. Commun.
329
869-872
2005
Arabidopsis thaliana
Manually annotated by BRENDA team
Murphy, T.M.; Guo, Y.Y.
Antimutagenic specificities of two plant glycosylases, oxoguanine glycosylase and formamidopyrimidine glycosylase, assayed in vivo
Biochem. Biophys. Res. Commun.
392
335-339
2010
Arabidopsis thaliana (O80358), Arabidopsis thaliana
Manually annotated by BRENDA team
Kathe, S.D.; Barrantes-Reynolds, R.; Jaruga, P.; Newton, M.R.; Burrows, C.J.; Bandaru, V.; Dizdaroglu, M.; Bond, J.P.; Wallace, S.S.
Plant and fungal Fpg homologs are formamidopyrimidine DNA glycosylases but not 8-oxoguanine DNA glycosylases
DNA Repair
8
643-653
2009
Arabidopsis thaliana (Q88AH6), Arabidopsis thaliana, Candida albicans
Manually annotated by BRENDA team
Duclos, S.; Aller, P.; Jaruga, P.; Dizdaroglu, M.; Wallace, S.S.; Doublie, S.
Structural and biochemical studies of a plant formamidopyrimidine-DNA glycosylase reveal why eukaryotic Fpg glycosylases do not excise 8-oxoguanine
DNA Repair
11
714-725
2012
Arabidopsis thaliana (O80358), Arabidopsis thaliana
Manually annotated by BRENDA team