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Information on EC 3.2.2.22 - rRNA N-glycosylase and Organism(s) Viscum album and UniProt Accession P81446

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EC Tree
     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.22 rRNA N-glycosylase
IUBMB Comments
Ricin A-chain and related toxins show this activity. Naked rRNA is attacked more slowly than rRNA in intact ribosomes. Naked rRNA from Escherichia coli is cleaved at a corresponding position.
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This record set is specific for:
Viscum album
UNIPROT: P81446
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Word Map
The taxonomic range for the selected organisms is: Viscum album
The enzyme appears in selected viruses and cellular organisms
Synonyms
pd-l1, ricin, shiga toxin, saporin, abrin, ricinus communis agglutinin, gelonin, ricin a chain, trichosanthin, ribosome-inactivating proteins, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cytotoxic ribosome-inactivating lectin
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gelonin
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-
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Mirabilis antiviral protein
-
-
-
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mistletoe lectin I
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mistletoe lectin II
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-
mistletoe lectin III
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-
ML-I
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MLII
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momorcochin-S
-
-
-
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nigrin b
-
-
-
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PGase
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polynucleotide:adenosine glycosidase
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-
ribosomal ribonucleate N-glycosidase
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-
-
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ribosome-inactivating type II protein
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ricin
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-
-
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RIPII
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RNA N-glycosidase
-
-
-
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rRNA N-glycosidase
saporins
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-
-
-
type 2 RIP
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type II ribosome-inactivating protein
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type II RIP
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VAA-I
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viscinum
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Viscum album agglutinin I
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-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolysis of the N-glycosylic bond at A-4324 in 28S rRNA from rat ribosomes
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of N-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
rRNA N-glycohydrolase
Ricin A-chain and related toxins show this activity. Naked rRNA is attacked more slowly than rRNA in intact ribosomes. Naked rRNA from Escherichia coli is cleaved at a corresponding position.
CAS REGISTRY NUMBER
COMMENTARY hide
113756-12-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
rRNA + H2O
?
show the reaction diagram
-
-
-
?
DNA + H2O
?
show the reaction diagram
-
primarily DNA substrate, polynucleotide:adenosine glycosidase activity
-
-
?
large rRNA + H2O
?
show the reaction diagram
-
the enzyme highly inhibits protein synthesis by ribosome inactivation via depurination of rRNA at a specific site
-
-
?
naked rRNA + H2O
?
show the reaction diagram
-
primarily DNA substrate, polynucleotide:adenosine glycosidase activity
-
-
?
polyA + H2O
?
show the reaction diagram
-
primarily DNA substrate, polynucleotide:adenosine glycosidase activity
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
rRNA + H2O
?
show the reaction diagram
-
-
-
?
large rRNA + H2O
?
show the reaction diagram
-
the enzyme highly inhibits protein synthesis by ribosome inactivation via depurination of rRNA at a specific site
-
-
?
additional information
?
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
target cell recognition and binding specificity for glycoproteins and gangliosides from different target cell types, detailed overview
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
mistletoe
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
-
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ML1_VISAL
564
0
62628
Swiss-Prot
Mitochondrion (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28478
-
1 * 28478, ML-I subunit A + 1 * 28981, ML-I subunits B, sequence calculation
28981
-
1 * 28478, ML-I subunit A + 1 * 28981, ML-I subunits B, sequence calculation
30000
-
1 * 30000, recombinant B-subunit, the enzyme forms a heterodimer comprising subunits A and B, the latter is the carbohydrate-binding subunit
66000
-
holoenzyme, native PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
additional information
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
high quality crystals of the ML-I complex obtained by vapor diffusion
homology modeling of both A and B chain protein and comparison with European mistletoe lectin-1, Himalayan mistletoe lectin, and ricin
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native ML-I from plant extract by ammonium sulfate fractionation, and affinity chromatography
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recombinant B-subunit from Escherichia coli strain BL21(DE3) inclusion bodies to homogeneity
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression of MLI and MLIII B-subunits in Escherichia coli strain BL21(DE3)
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expression of both the A and the B chains of KML separately in a bacterial expression system
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isozyme MLIII, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, expression of the B-subunit in Escherichia coli strain BL21(DE3) in inclusion bodies
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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
recombinant B-subunit from Escherichia coli strain BL21(DE3) inclusion bodies by denaturation in 7 M guanidinium hydrochloride, renaturation by 130fld dilution in phosphate buffer, pH 7.4, and incubation for 16 h at 4°C
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renaturation of recombinant MLIII B-subunit from 7 M guanidinium hydrochloride by dilution with phosphate buffer, pH 7.4, and incubation for 16 h at 4°C
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Krauspenhaar, R.; Rypniewski, W.; Kalkura, N.; Moore, K.; DeLucas, L.; Stoeva, S.; Mikhailov, A.; Voelter, W.; Betzel, C.
Crystallization under microgravity of mistletoe lectin I from Viscum album with adenine monophosphate and the crystal structure at 1.9.ANG. resolution
Acta Crystallogr. Sect. D
58
1704-1707
2002
Viscum album (P81446), Viscum album
Manually annotated by BRENDA team
Muething, J.; Meisen, I.; Bulau, P.; Langer, M.; Witthohn, K.; Lentzen, H.; Neumann, U.; Peter-Katalinic, J.
Mistletoe lectin I is a sialic acid-specific lectin with strict preference to gangliosides and glycoproteins with terminal Neu5Ac alpha 2-6Gal beta 1-4GlcNAc residues
Biochemistry
43
2996-3007
2004
Viscum album
Manually annotated by BRENDA team
Pevzner, I.B.; Agapov, II; Pfueller, U.; Pfueller, K.; Maluchenko, N.V.; Moisenovich, M.M.; Tonevitsky, A.G.; Kirpichnikov, M.P.
Cloning and expression of mistletoe lectin III B-subunit
Biochemistry (Moscow)
70
306-315
2005
Viscum album
Manually annotated by BRENDA team
Pevzner, I.B.; Agapov, II; Niwa, H.; Maluchenko, N.V.; Moisenovich, M.M.; Pfuller, U.; Tonevitsky, A.G.
Differences in amino acid sequences of mistletoe lectin I and III B-subunits determining carbohydrate binding specificity
Biochim. Biophys. Acta
1675
155-164
2004
Viscum album
Manually annotated by BRENDA team
Hartley, M.R.; Lord, J.M.
Cytotoxic ribosome-inactivating lectins from plants
Biochim. Biophys. Acta
1701
1-14
2004
Abrus precatorius, Cucurbita pepo, Dianthus caryophyllus, Escherichia coli, Mirabilis jalapa, Momordica charantia, Phytolacca americana, Sambucus nigra, Saponaria officinalis, Shigella dysenteriae, Trichosanthes kirilowii, Triticum aestivum, Viscum album, Adenia volkensii, Sambucus ebulus, Ricinus communis (P02879)
Manually annotated by BRENDA team
Stirpe, F.
Ribosome-inactivating proteins
Toxicon
44
371-383
2004
Abrus precatorius, Ricinus communis, Suregada multiflora, Hordeum vulgare, Phytolacca americana, Sambucus nigra, Saponaria officinalis, Shigella dysenteriae, Trichosanthes kirilowii, Viscum album, Zea mays, Phoradendron californicum, Adenia volkensii, Adenia digitata, Mirabilis expansa
Manually annotated by BRENDA team
Ye, W.; Nanga, R.P.; Kang, C.B.; Song, J.H.; Song, S.K.; Yoon, H.S.
Molecular characterization of the recombinant A-chain of a type II ribosome-inactivating protein (RIP) from Viscum album coloratum and structural basis on its ribosome-inactivating activity and the sugar-binding properties of the B-chain
J. Biochem. Mol. Biol.
39
560-570
2006
Viscum album
Manually annotated by BRENDA team