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EC Tree
IUBMB Comments Ricin A-chain and related toxins show this activity. Naked rRNA is attacked more slowly than rRNA in intact ribosomes. Naked rRNA from Escherichia coli is cleaved at a corresponding position.
The taxonomic range for the selected organisms is: Viscum album The enzyme appears in selected viruses and cellular organisms
Synonyms
pd-l1, ricin, shiga toxin, saporin, abrin, ricinus communis agglutinin, gelonin, ricin a chain, trichosanthin, ribosome-inactivating proteins,
more
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cytotoxic ribosome-inactivating lectin
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Mirabilis antiviral protein
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polynucleotide:adenosine glycosidase
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ribosomal ribonucleate N-glycosidase
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ribosome-inactivating type II protein
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RNA N-glycosidase
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type II ribosome-inactivating protein
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Viscum album agglutinin I
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rRNA N-glycosidase
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hydrolysis of the N-glycosylic bond at A-4324 in 28S rRNA from rat ribosomes
hydrolysis of the N-glycosylic bond at A-4324 in 28S rRNA from rat ribosomes
catalytic mechanism
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hydrolysis of the N-glycosylic bond at A-4324 in 28S rRNA from rat ribosomes
substrate recogition via glycoprotein binding, overview
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hydrolysis of N-glycosyl bond
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rRNA N-glycohydrolase
Ricin A-chain and related toxins show this activity. Naked rRNA is attacked more slowly than rRNA in intact ribosomes. Naked rRNA from Escherichia coli is cleaved at a corresponding position.
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DNA + H2O
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primarily DNA substrate, polynucleotide:adenosine glycosidase activity
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large rRNA + H2O
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the enzyme highly inhibits protein synthesis by ribosome inactivation via depurination of rRNA at a specific site
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naked rRNA + H2O
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primarily DNA substrate, polynucleotide:adenosine glycosidase activity
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polyA + H2O
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primarily DNA substrate, polynucleotide:adenosine glycosidase activity
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additional information
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additional information
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the enzyme binds specifically to terminal Gal and GalNAc of the target cell surface receptor
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additional information
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the enzyme inhibits protein synthesis of the targeted cells and is highly cytotoxic, viscumin causes apoptosis
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additional information
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ML-I is a sialic acid-specific lectin with strict preference to gangliosides and glycoproteins with terminal Neu5Ac alpha 2-6Gal beta 1-4GlcNAc residues, e.g. on human granulocytes or erythrocytes, structural features, overview
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additional information
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in vitro synthesis of luciferase in a rabbit reticulocyte lysate system is inhibited by enzyme A chain protein. Enzyme A chain as well as native enzyme inhibits the growth of MCF7/caspases-3 (+) cells. Key catalytic residues are Tyr76, Tyr115, Glu165, and Arg168
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large rRNA + H2O
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the enzyme highly inhibits protein synthesis by ribosome inactivation via depurination of rRNA at a specific site
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additional information
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additional information
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the enzyme binds specifically to terminal Gal and GalNAc of the target cell surface receptor
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additional information
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the enzyme inhibits protein synthesis of the targeted cells and is highly cytotoxic, viscumin causes apoptosis
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additional information
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target cell recognition and binding specificity for glycoproteins and gangliosides from different target cell types, detailed overview
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mistletoe
Uniprot
brenda
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brenda
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brenda
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ML1_VISAL
564
0
62628
Swiss-Prot
Mitochondrion (Reliability: 2 )
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28478
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1 * 28478, ML-I subunit A + 1 * 28981, ML-I subunits B, sequence calculation
28981
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1 * 28478, ML-I subunit A + 1 * 28981, ML-I subunits B, sequence calculation
30000
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1 * 30000, recombinant B-subunit, the enzyme forms a heterodimer comprising subunits A and B, the latter is the carbohydrate-binding subunit
66000
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holoenzyme, native PAGE
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dimer
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1 * 28478, ML-I subunit A + 1 * 28981, ML-I subunits B, sequence calculation
dimer
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1 * 30000, recombinant B-subunit, the enzyme forms a heterodimer comprising subunits A and B, the latter is the carbohydrate-binding subunit
dimer
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heterodimer formed by a catalytic subunit A and a carbohydrate binding subunit B
dimer
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subunit A and subunit B connected by a disulfide bridge
additional information
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amino acid sequence analysis and comparison with other toxin sequences, overview
additional information
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structure comparison and analysis
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high quality crystals of the ML-I complex obtained by vapor diffusion
homology modeling of both A and B chain protein and comparison with European mistletoe lectin-1, Himalayan mistletoe lectin, and ricin
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native ML-I from plant extract by ammonium sulfate fractionation, and affinity chromatography
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recombinant B-subunit from Escherichia coli strain BL21(DE3) inclusion bodies to homogeneity
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DNA and amino acid sequence determination and analysis, expression of MLI and MLIII B-subunits in Escherichia coli strain BL21(DE3)
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expression of both the A and the B chains of KML separately in a bacterial expression system
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isozyme MLIII, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, expression of the B-subunit in Escherichia coli strain BL21(DE3) in inclusion bodies
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recombinant B-subunit from Escherichia coli strain BL21(DE3) inclusion bodies by denaturation in 7 M guanidinium hydrochloride, renaturation by 130fld dilution in phosphate buffer, pH 7.4, and incubation for 16 h at 4°C
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renaturation of recombinant MLIII B-subunit from 7 M guanidinium hydrochloride by dilution with phosphate buffer, pH 7.4, and incubation for 16 h at 4°C
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Krauspenhaar, R.; Rypniewski, W.; Kalkura, N.; Moore, K.; DeLucas, L.; Stoeva, S.; Mikhailov, A.; Voelter, W.; Betzel, C.
Crystallization under microgravity of mistletoe lectin I from Viscum album with adenine monophosphate and the crystal structure at 1.9.ANG. resolution
Acta Crystallogr. Sect. D
58
1704-1707
2002
Viscum album (P81446), Viscum album
brenda
Muething, J.; Meisen, I.; Bulau, P.; Langer, M.; Witthohn, K.; Lentzen, H.; Neumann, U.; Peter-Katalinic, J.
Mistletoe lectin I is a sialic acid-specific lectin with strict preference to gangliosides and glycoproteins with terminal Neu5Ac alpha 2-6Gal beta 1-4GlcNAc residues
Biochemistry
43
2996-3007
2004
Viscum album
brenda
Pevzner, I.B.; Agapov, II; Pfueller, U.; Pfueller, K.; Maluchenko, N.V.; Moisenovich, M.M.; Tonevitsky, A.G.; Kirpichnikov, M.P.
Cloning and expression of mistletoe lectin III B-subunit
Biochemistry (Moscow)
70
306-315
2005
Viscum album
brenda
Pevzner, I.B.; Agapov, II; Niwa, H.; Maluchenko, N.V.; Moisenovich, M.M.; Pfuller, U.; Tonevitsky, A.G.
Differences in amino acid sequences of mistletoe lectin I and III B-subunits determining carbohydrate binding specificity
Biochim. Biophys. Acta
1675
155-164
2004
Viscum album
brenda
Hartley, M.R.; Lord, J.M.
Cytotoxic ribosome-inactivating lectins from plants
Biochim. Biophys. Acta
1701
1-14
2004
Abrus precatorius, Cucurbita pepo, Dianthus caryophyllus, Escherichia coli, Mirabilis jalapa, Momordica charantia, Phytolacca americana, Sambucus nigra, Saponaria officinalis, Shigella dysenteriae, Trichosanthes kirilowii, Triticum aestivum, Viscum album, Adenia volkensii, Sambucus ebulus, Ricinus communis (P02879)
brenda
Stirpe, F.
Ribosome-inactivating proteins
Toxicon
44
371-383
2004
Abrus precatorius, Ricinus communis, Suregada multiflora, Hordeum vulgare, Phytolacca americana, Sambucus nigra, Saponaria officinalis, Shigella dysenteriae, Trichosanthes kirilowii, Viscum album, Zea mays, Phoradendron californicum, Adenia volkensii, Adenia digitata, Mirabilis expansa
brenda
Ye, W.; Nanga, R.P.; Kang, C.B.; Song, J.H.; Song, S.K.; Yoon, H.S.
Molecular characterization of the recombinant A-chain of a type II ribosome-inactivating protein (RIP) from Viscum album coloratum and structural basis on its ribosome-inactivating activity and the sugar-binding properties of the B-chain
J. Biochem. Mol. Biol.
39
560-570
2006
Viscum album
brenda