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Information on EC 3.2.2.17 - deoxyribodipyrimidine endonucleosidase
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EC Tree
3.2.2.17 deoxyribodipyrimidine endonucleosidaseSpecify your search results
Word Map
- 3.2.2.17
- micrococcus
-
uv-irradiated
- photoproducts
-
apyrimidinic
-
photoreactivation
-
incises
- xeroderma
- glycosylases
-
apurinic
-
excision-repair
-
endonuclease-sensitive
-
dimer-dna
-
uvrabc
-
formamidopyrimidine-dna
- bursaria
- ap-endonuclease
-
enzyme-dna
-
photorepaired
- analysis
- medicine
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
uv endonuclease, t4 uv endonuclease, t4-pdg, uv-specific endonuclease, pyrimidine dimer glycosylase, t4 pyrimidine dimer glycosylase, chlorella virus pyrimidine dimer glycosylase, pd-dna glycosylase, pyrimidine dimer dna glycosylase, pd-glycosylase, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Chlorella virus pyrimidine dimer glycosylase
cis-syn trans-syn-II cyclobutane pyrimidine dimer glycosylase
-
-
denV
deoxyribonucleate pyrimidine dimer glycosidase
-
-
-
-
deoxyribopyrimidine endonucleosidase
-
-
-
-
dimer dependent endonuclease V
-
-
-
-
endodeoxyribonuclease (apurinic or apyrimidinic)
Tequatrovirus T4
-
formerly designated EC 2.1.25.2
endodeoxyribonuclease (pyrimidine dimer)
Tequatrovirus T4
-
formerly designated EC 3.1.25.1
endonuclease V
PD-DNA glycosylase
-
-
-
-
pyrimidine dimer DNA glycosylase
-
-
-
-
pyrimidine dimer-specific glycosylase/AP lyase
-
-
T4 Endo V
T4 PD-DNA-glycosylase
T4 pyrimidine dimer glycosylase
T4 UV endonuclease
Chlorella virus pyrimidine dimer glycosylase
-
-
denV
Tequatrovirus T4 T4denV+
-
-
-
denV
Tequatrovirus T4 T4denV1
-
-
-
endonuclease V
Tequatrovirus T4 T4denV+
-
-
-
T4 Endo V
-
-
-
-
T4 PD-DNA-glycosylase
Tequatrovirus T4 T4denV1
-
-
-
T4 UV endonuclease
Tequatrovirus T4 T4denV+
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
thymidine-dimer-containing DNA + H2O = thymidine-dimer-containing DNA with apyrimidinic site
thymidine-dimer-containing DNA + H2O = thymidine-dimer-containing DNA with apyrimidinic site
-
-
-
-
thymidine-dimer-containing DNA + H2O = thymidine-dimer-containing DNA with apyrimidinic site
hydrolysis of phosphodiester bonds
Tequatrovirus T4
-
thymidine-dimer-containing DNA + H2O = thymidine-dimer-containing DNA with apyrimidinic site
cleaves N-glycosyl bond between pyrimidine and sugar without rupture of a phosphodiester bond
-
thymidine-dimer-containing DNA + H2O = thymidine-dimer-containing DNA with apyrimidinic site
scheme of reaction mechanism
Tequatrovirus T4
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of N-glycosyl bond
hydrolysis of N-glycosyl bond
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
deoxy-D-ribocyclobutadipyrimidine polynucleotidodeoxyribohydrolase
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
16-mers oligodeoxynucleotide + H2O
?
-
anomers of methyl-deoxyribofuranose analogues indicates that T4-pdg preferentially cleaved the beta-anomers
-
-
?
poly(dAdT) + H2O
oligonucleotides
Tequatrovirus T4
-
-
acid-soluble oligonucleotides, length less than 15 nucleotides
?
DNA + H2O
?
-
the combined rate of sugar ring opening and reduction of the sugar is significantly slower than the rate of formation of (T4-pdg)-DNA intermediate
-
-
?
DNA + H2O
?
-
pyrimidine cyclobutane dimer formed between adjacent pyrimidines
-
-
?
DNA + H2O
?
Tequatrovirus T4
-
the enzyme incises DNA at cyclobutane pyrimidine dimers that are formed as a consequence of exposure to ultraviolet light. The investigation reveals a temporal uncoupling of the T4-Pdg-induced DNA bending and nucleotide flipping
-
-
?
DNA + H2O
thymine + ?
-
enzyme mechanism glycosylase/AP lyase, cis-syn and trans-synII-isomers of cyclobutane pyrimidine dimers in double-stranded DNA
-
-
?
DNA + H2O
thymine + ?
-
cis-syn pyrimidine dimer specific
-
-
?
DNA + H2O
thymine + ?
Tequatrovirus T4
-
beta-elimination reaction results in an alpha,beta-unsaturated aldehyde
-
-
?
DNA + H2O
thymine + ?
Tequatrovirus T4
-
strict specificity for DNA cyclobutyldipyrimidines
-
-
?
DNA + H2O
thymine + ?
Tequatrovirus T4
-
double stranded DNA containing pyrimidine dimers
-
-
?
DNA + H2O
thymine + ?
Tequatrovirus T4
-
UV-irradiated DNA, containing thymine dimers
enzymatic photoreactivation, direct photoreversal, releases free thymine from E. coli DNA
?
DNA phiX174 RFI + H2O
thymine + ?
-
irradiated with increasing doses of UV light
-
-
?
DNA phiX174 RFI + H2O
thymine + ?
-
double-stranded covalently closed circular DNA molecule contains apurinic sites
-
?
oligonucleotide + H2O
?
-
49-mers containing a single site-specific cis-syn, trans-syn I or trans-syn II thymine dimer
beta elimination reaction results in products wich have 3'4-hydroxy-2-pentanal ends
-
?
oligonucleotide + H2O
?
-
49-mers radiolabeled containing a site-specific uracil with uracil DNA glycosylase
incise of AP sites resulting in beta- and delta-elimination products with 3'4-hydroxy-2-pentenal and 3'-phosphate termini respectively
-
?
oligonucleotide + H2O
?
-
containing either a (6-4) or Dewar photoproduct of TpT
-
-
?
oligonucleotides + H2O
dCMP + dTMP
-
49mers containing a site specific cis-syn, trans-syn I or trans-syn II thymine dimer, beta-elimination reaction
-
-
?
additional information
?
-
-
unable to incise DNA containing either A:G, A:8-oxoG or 8-oxoG:C mismatches
-
-
?
additional information
?
-
-
3'nicking is not result of the hydrolysis of the phospho ester bond, but rather result of a beta-elimination reaction, the sequence of the 138 amino acid residues of T4 UV endonuclease is known, active sites for DNA glycosylase and the nicking activity seem to be independent, since endonuclease is a phosphoric diester hydrolase classified as EC 3.1.4.-T4 UV endonuclease and Micrococcus luteus endonuclease are not endonucleases
-
-
?
additional information
?
-
-
UV-specific endonuclease inactive on UV single-stranded DNA, N-glycosylase activity less active on single-stranded substrate
-
-
?
additional information
?
-
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
Tequatrovirus T4
-
3'nicking is not result of the hydrolysis of the phospho ester bond, but rather result of a beta-elimination reaction, the sequence of the 138 amino acid residues of T4 UV endonuclease is known, active sites for DNA glycosylase and the nicking activity seem to be independent, since endonuclease is a phosphoric diester hydrolase classified as EC 3.1.4.-T4 UV endonuclease and Micrococcus luteus endonuclease are not endonucleases
-
-
?
additional information
?
-
Tequatrovirus T4
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
Tequatrovirus T4
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
Tequatrovirus T4
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
Tequatrovirus T4
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
Tequatrovirus T4
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
Tequatrovirus T4
-
the enzyme reduces the incidence of basal-cell and squamous cell carcinoma in mice. Treatment of Cdk4(R24C/R24C)/Nras(Q61K) mice topically with the T4 endonuclease V DNA repair enzyme immediately prior to neonatal ultraviolet radiation (UVR) exposure has a powerful effect in exacerbating melanoma development, overview
-
-
?
additional information
?
-
Tequatrovirus T4 T4denV+
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
DNA + H2O
?
-
pyrimidine cyclobutane dimer formed between adjacent pyrimidines
-
-
?
additional information
?
-
-
UV-specific endonuclease inactive on UV single-stranded DNA, N-glycosylase activity less active on single-stranded substrate
-
-
?
additional information
?
-
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
Tequatrovirus T4
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
Tequatrovirus T4
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
Tequatrovirus T4
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
Tequatrovirus T4
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
Tequatrovirus T4
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
additional information
?
-
Tequatrovirus T4
-
the enzyme reduces the incidence of basal-cell and squamous cell carcinoma in mice. Treatment of Cdk4(R24C/R24C)/Nras(Q61K) mice topically with the T4 endonuclease V DNA repair enzyme immediately prior to neonatal ultraviolet radiation (UVR) exposure has a powerful effect in exacerbating melanoma development, overview
-
-
?
additional information
?
-
Tequatrovirus T4 T4denV+
-
only nicking of UV-irradiated DNA, creating phospho diester bond breaks 5' with respect to pyrimidine dimers, protein containing the glycosylase activity also contains the apyrimidinic endonuclease activity, multifunctional enzyme, 2 distinct domains in a single enzyme molecule possess both pyrimidine dimer-DNA glycosylase, and AP endonuclease activity, first the glycosylase cleaves glycosylic bond of one of the pyrimidine residues of a dimer to yield an AP site, then AP endonuclease hydrolyzes the phospho diester bond near the AP site, prefers duplex DNA to single-stranded
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
MgCl2
NaCl
MgCl2
-
catalytic activity 7 times greater than in presence of EDTA, DNA glycosylase activity preceded by a Mg2+ dependent AP endonuclease, followed by an AP endonuclease active in the absence of Mg2+ and in presence of EDTA
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-[N-(acetoxyacetyl)amino]fluorene
Tequatrovirus T4
-
AAAF, carcinogen , extensive modification of DNA does not significantly reduce the thymine dimer yield
DNA
-
depurinated DNA competitively inhibits the UV endonuclease activity, irradiated DNA inhibits the AP endonuclease activity
NaBH4
-
The rate of the covalent imine intermediate suggest that the reduction of the intermediate is rate-limiting in the reaction
NaBH4
-
reduces the imino intermediate resulting in a stable covalent linkage between the enzyme and the DNA
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
isopropyl-1-thio-beta-D-galactopyranoside
-
induction of the pdg gene
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Carcinoma
Enhancement of DNA repair using topical T4 endonuclease V does not inhibit melanoma formation in Cdk4/Tyr-Nras mice following neonatal UVR.
Carcinoma, Squamous Cell
Enhancement of DNA repair using topical T4 endonuclease V does not inhibit melanoma formation in Cdk4/Tyr-Nras mice following neonatal UVR.
Ichthyosis
Restoration of u.v.-induced excision repair in Xeroderma D cells transfected with the denV gene of bacteriophage T4.
Infections
Are pyrimidine dimers tolerated during DNA replication of UV-irradiated parvovirus minute-virus-of-mice in mouse fibroblasts?
Melanoma
Enhancement of DNA repair using topical T4 endonuclease V does not inhibit melanoma formation in Cdk4/Tyr-Nras mice following neonatal UVR.
Plasmacytoma
Mammalian mitochondrial endonuclease activities specific for ultraviolet-irradiated DNA.
Skin Neoplasms
Enhancement of DNA repair using topical T4 endonuclease V does not inhibit melanoma formation in Cdk4/Tyr-Nras mice following neonatal UVR.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.001212
DNA
-
excision of 4,6-diamino-5-formamidopyrimidine
0.002556
DNA
-
excision of 2,6-diamino-4-hydroxy-5-formamidopyrimidine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
cis-syn 49mer 0.09 nM, trans-syn II 49mer 1.5 nM, AP 49mer 0.17 nM
additional information
Tequatrovirus T4
-
cis-syn 49mer 0.19 nM, trans-syn II 49mer 6.0 nM, AP 49mer 0.15 nM
additional information
Tequatrovirus T4
-
20000 U/mg. One unit of rT4 endo V activity is defined as the amount of rT4 endo V used to completely relax 250 ng of UV-irradiated plasmid DNA in 30 min at 37°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 8
6.5
6 - 8
-
pH optimum for the 2 enzymatic activities differs, AP lyase function at pH 6.5, becoming inactive at pH 8
6 - 8
Tequatrovirus T4
-
pH optimum for the 2 enzymatic activities differs, AP lyase function at pH 6.5, becoming inactive at pH 8
6.5
Tequatrovirus T4
-
2 distinct domains within the single polypeptide, the 2 activities possess different optima for pH and salt concentration and different degrees of thermolability
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 8.5
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
15 - 37
15 - 37
Tequatrovirus T4
-
possibility of denaturation of the substrate at temperatures above 20°C
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Tequatrovirus T4
42 Chlorella viruses, host alga Chlorella strain NC64A
-
-
brenda
eukaryotic algal virus, Chlorella virus, PBCV-1
-
-
brenda
Tequatrovirus T4
-
170697, 170698, 170703, 171803, 171805, 171807, 171808, 171809, 171810, 171812, 171813, 656231, 678169, 679415, 681339, 681394, 682057, 682201, 682279, 710256
-
-
brenda
Tequatrovirus T4
T4 D, T4v1, T4uvs-5, T4uvs-13, host Escherichia coli B, Escherichia coli 1100, Escherichia coli Cr63
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). END5_BPT4
138
0
16079
Swiss-Prot
other Location (Reliability: 3)
A0A858I7T9_9CAUD
138
0
16155
TrEMBL
other Location (Reliability: 3)
A0A3G2KBF4_9CAUD
137
0
16103
TrEMBL
other Location (Reliability: 2)
A0A5Q2W7N5_9CAUD
137
0
16151
TrEMBL
other Location (Reliability: 3)
A0A6H0X9V2_9CAUD
138
0
16537
TrEMBL
other Location (Reliability: 2)
E0STL1_IGNAA
Ignisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1)
143
0
17336
TrEMBL
-
D5U3B9_THEAM
Thermosphaera aggregans (strain DSM 11486 / M11TL)
172
0
20048
TrEMBL
-
A0A249XZ41_9CAUD
138
0
16298
TrEMBL
other Location (Reliability: 3)
Q0AH64_NITEC
Nitrosomonas eutropha (strain DSM 101675 / C91 / Nm57)
146
0
16505
TrEMBL
-
A0A6H0X754_9CAUD
138
0
16537
TrEMBL
other Location (Reliability: 2)
Q3J702_NITOC
Nitrosococcus oceani (strain ATCC 19707 / BCRC 17464 / JCM 30415 / NCIMB 11848 / C-107)
155
0
17758
TrEMBL
-
A0A3G2KC26_9CAUD
137
0
16047
TrEMBL
other Location (Reliability: 3)
I4D677_DESAJ
Desulfosporosinus acidiphilus (strain DSM 22704 / JCM 16185 / SJ4)
135
0
15749
TrEMBL
-
A0A8G0QJU9_9CAUD
138
0
16366
TrEMBL
other Location (Reliability: 3)
A0A653FZP2_9CAUD
138
0
16298
TrEMBL
other Location (Reliability: 3)
B2GIR7_KOCRD
Kocuria rhizophila (strain ATCC 9341 / DSM 348 / NBRC 103217 / DC2201)
149
0
16867
TrEMBL
-
G8TZR6_SULAD
Sulfobacillus acidophilus (strain ATCC 700253 / DSM 10332 / NAL)
121
0
14395
TrEMBL
-
A0A6H0X8F4_9CAUD
138
0
16594
TrEMBL
other Location (Reliability: 2)
E4RUY4_LEAB4
Leadbetterella byssophila (strain DSM 17132 / JCM 16389 / KACC 11308 / NBRC 106382 / 4M15)
140
0
16488
TrEMBL
-
A5IMN0_THEP1
Thermotoga petrophila (strain ATCC BAA-488 / DSM 13995 / JCM 10881 / RKU-1)
129
0
15567
TrEMBL
-
A0A899IMV4_9CAUD
137
0
15963
TrEMBL
other Location (Reliability: 3)
LYSC_HUMAN
148
0
16537
Swiss-Prot
other Location (Reliability: 3)
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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