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Information on EC 3.2.1.99 - arabinan endo-1,5-alpha-L-arabinanase
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EC Tree
3.2.1.99 arabinan endo-1,5-alpha-L-arabinanaseIUBMB Comments
Acts best on linear 1,5-alpha-L-arabinan. Also acts on branched arabinan, but more slowly.
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Word Map
- 3.2.1.99
- arabinofuranosidase
- alpha-l-arabinofuranosidase
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debranched
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arabinases
- arabinotriose
- arabino-oligosaccharides
- nutrition
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magnetospheric
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endo-galactanase
- l-arabitol
- analysis
- rhamnogalacturonans
- lipoarabinomannans
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arabinose-containing
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exo-acting
- thermodenitrificans
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five-bladed
- p-nitrophenyl-alpha-l-arabinofuranoside
- cellvibrio
- biofuel production
- degradation
- synthesis
- industry
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
endo-arabinanase, endoarabinanase, arase, endo-arabinase, abn-ts, abns1, abnase, endo-1,5-alpha-l-arabinanase, arb43a, cedaase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
ABN
Abn1
Abn2
ABNA
abnA1
abnA2
ABNase
Abnc
AbnS1
AbnZ1
alpha-1,5-L-endo-arabinanase
alpha-L-arabinohydrolase
ARA1
araban 5-alpha-L-arabinohydrolase
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arabinase, endo-1,5-alpha-L-
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BlAbn1
cold-adapted endo-arabinanase
endo-(1,5)-alpha-L-arabinanase
endo-(1->5)-alpha-L-arabinanase
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endo-1,5-alpha-arabinanase
endo-1,5-alpha-L-arabinanase
endo-1,5-alpha-L-arabinase
endo-1,5-arabinase
endo-alpha-(1->5)-L-arabinanase
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endo-alpha-1,5-arabanase
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endo-alpha-1,5-L-arabinanase
endo-arabanase
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endo-arabinanase
endo-arabinase
endo-L-arabinanase
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endoarabinanase
endoarabinase
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GH43 arabinanase
GH43 endo-arabinanase
Tpet_0637
additional information
endo-1,5-alpha-arabinanase
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endo-1,5-alpha-L-arabinanase
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endo-1,5-alpha-L-arabinase
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endo-arabinanase
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endo-arabinase
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additional information
the enzyme belongs to the glycoside hydrolase family 43, GH43
additional information
the enzyme belongs to the glycoside hydrolase family 43, GH43
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additional information
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the enzyme belongs to the glycoside hydrolase family 43, GH43
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O = alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O = alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
regulation, induction and carbon catabolite repression of endo-1,5-alpha-arabinanase
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alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O = alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
regulation, induction and carbon catabolite repression of endo-1,5-alpha-arabinanase
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alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O = alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
regulation, induction and carbon catabolite repression of endo-1,5-alpha-arabinanase
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alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O = alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
also acts slowly on beet-arabinan
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alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O = alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
three catalytic carboxylates: Asp27, the general base, Glu201, the general acid, and Asp147, the pKa modulator, substrate binding structure and catalytic mechanism, overview
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O = alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
regulation, induction and carbon catabolite repression of endo-1,5-alpha-arabinanase
Aspergillus niger N400 / DSM 2466 / ATCC 9029 / CBS 120.49
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alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O = alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
regulation, induction and carbon catabolite repression of endo-1,5-alpha-arabinanase
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alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O = alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
three catalytic carboxylates: Asp27, the general base, Glu201, the general acid, and Asp147, the pKa modulator, substrate binding structure and catalytic mechanism, overview
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
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SYSTEMATIC NAME
IUBMB Comments
5-alpha-L-arabinan 5-alpha-L-arabinanohydrolase
Acts best on linear 1,5-alpha-L-arabinan. Also acts on branched arabinan, but more slowly.
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CAS REGISTRY NUMBER
COMMENTARY
75432-96-1
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(1->5)-L-alphaarabinan + H2O
(1->5)-L-arabinooligosaccharides
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?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
1,5-alpha-L-arabinan + H2O
L-arabinose + alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
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?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
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?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
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?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranose
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?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
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?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
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DP6
composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times, product formation, end-products after exhaustive enzymatic degradation, intermediate enzymatic hydrolysis: L-arabinobiose + L-arabinotetraose and L-arabinotriose
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
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DP7
composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times, product formation, end-products after exhaustive enzymatic degradation, intermediate enzymatic hydrolysis: L-arabinotriose + L-arabinotetraose
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-arabinofuranose
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?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
branched arabinan + H2O
?
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branched arabinan from sugar beet. Hydrolyzed at 12% compared to debranched arabinan
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?
cell wall polysaccharide + H2O
arabinose + galactose + rhamnose + fucose
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F-11, apple
F-11, 34.3% arabinose + 10% galactose + 2.6% rhamnose/fucose
?
debranched (1->5)-L-arabinan + H2O
L-arabinose
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under the optimum conditions, 16 g/l L-arabinose is obtained from 20 g/l debranched arabinan with a hydrolysis yield of 80%
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?
debranched (1->5)-L-arabinan + H2O
L-arabinose + L-arabinosyl-alpha-(1->5)-L-arabinoside + ?
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debranched arabinan + H2O
arabinose + arabinobiose + arabinotriose
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?
reduced arabinohexaose + H2O
arabinotriose + reduced arabinotriose
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the enzyme hydrolyzes a branched arabinohexaose with a double substituted arabinose at subsite-2
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?
sugar beet arabinan + H2O
L-arabinose + L-arabinosyl-alpha-(1->5)-L-arabinoside
48% of the activity with debranched (1->5)-L-arabinan
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?
sugar beet arabinan arabinan + H2O
L-arabinose + ?
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conversion yield of 83% at pH 5.0 and 75°C for 216 h
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?
1,5-alpha-L-arabinan + H2O
?
Abn2, together with previously characterized AbnA, is responsible for the majority of the extracellular arabinan activity in Bacillus subtilis. Abn2 is monocistronic, and its expression is driven from two different promoters
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?
1,5-alpha-L-arabinan + H2O
?
Abn2, together with previously characterized AbnA, is responsible for the majority of the extracellular arabinan activity in Bacillus subtilis. Abn2 is monocistronic, and its expression is driven from two different promoters
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?
1,5-alpha-L-arabinan + H2O
?
debranched arabinan, best substrate
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?
1,5-alpha-L-arabinan + H2O
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + ?
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linear arabinan
main product
?
1,5-alpha-L-arabinan + H2O
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + ?
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linear arabinan
main product
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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major final end-products, upon exhaustive degradation of substrate some monomers, L-arabinose, are produced, action patterns of initial hydrolysis products
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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best substrate, endo mechanism
action patterns of initial hydrolysis products, product formation, overview
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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product formation, overview: oligoarabinosides with degree of polymerization between 2 and 5 are the major products, but only trace amounts of free arabinose
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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major final end-products, upon exhaustive degradation of substrate some monomers, L-arabinose, are produced, action patterns of initial hydrolysis products
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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best substrate, endo mechanism
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?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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best substrate, endo mechanism
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?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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best substrate, endo mechanism
major final end-products, upon exhaustive degradation of substrate some monomers, L-arabinose, are produced
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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best substrate, endo mechanism
action patterns of initial hydrolysis products, product formation, overview
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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best substrate: linear 1,5-alpha-L-haze arabinan
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?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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i.e. debranched arabinan
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?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
AB063312
i.e. debranched arabinan
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?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
AB063312
i.e. debranched arabinan
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?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
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?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
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?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
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F-11 and IFO 3022: best substrate, endo mechanism
end-product
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
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F-11 best substrate
end-product, F-11: releases arabinose, arabinobiose, arabinotriose and higher oligosaccharides during hydrolysis, end-product are arabinose and arabinobiose
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
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IFO 3134, linear 1,5-alpha-L-arabinan
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?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
Bacillus subtilis F-11
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?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
Bacillus subtilis F-11
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F-11 and IFO 3022: best substrate, endo mechanism
end-product
?
1,5-alpha-L-arabinan + H2O
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + L-arabinose
Bacillus subtilis F-11
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F-11 best substrate
end-product, F-11: releases arabinose, arabinobiose, arabinotriose and higher oligosaccharides during hydrolysis, end-product are arabinose and arabinobiose
?
1,5-alpha-L-arabinan + H2O
L-arabinose + alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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F-11 and IFO 3022: best substrate, endo mechanism
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-
?
1,5-alpha-L-arabinan + H2O
L-arabinose + alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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F-11 best substrate
-
-
?
1,5-alpha-L-arabinan + H2O
L-arabinose + alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
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IFO 3022, best substrate
IFO 3022
?
1,5-alpha-L-arabinan + H2O
L-arabinose + alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
Bacillus subtilis F-11
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F-11 and IFO 3022: best substrate, endo mechanism
-
-
?
1,5-alpha-L-arabinan + H2O
L-arabinose + alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara
Bacillus subtilis F-11
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F-11 best substrate
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-
?
1,5-alpha-L-arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside
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-
-
?
1,5-alpha-L-arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside
-
-
-
?
1,5-alpha-L-arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside + L-arabinose
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-
-
?
1,5-alpha-L-arabinan + H2O
oligo-alpha-(1->5)-L-arabinoside + L-arabinose
-
-
-
?
1,5-L-alpha-arabinoheptaose + H2O
1,5-L-alpha-arabinotriose + 1,5-L-alpha-arabinotetraose
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-
-
-
?
1,5-L-alpha-arabinoheptaose + H2O
1,5-L-alpha-arabinotriose + 1,5-L-alpha-arabinotetraose
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-
-
-
?
1,5-L-alpha-arabinopentaose + H2O
1,5-L-alpha-arabinotriose + 1,5-L-alpha-arabinobiose
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-
-
-
?
1,5-L-alpha-arabinopentaose + H2O
1,5-L-alpha-arabinotriose + 1,5-L-alpha-arabinobiose
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-
-
-
?
1,5-L-alpha-arabinotetraose + H2O
1,5-L-alpha-arabinobiose + 1,5-L-alpha-arabinobiose
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-
-
-
?
1,5-L-alpha-arabinotetraose + H2O
1,5-L-alpha-arabinobiose + 1,5-L-alpha-arabinobiose
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-
-
-
?
1,5-L-alpha-arabinotriose + H2O
L-arabinose + 1,5-L-alpha-arabinobiose
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-
-
-
?
1,5-L-alpha-arabinotriose + H2O
L-arabinose + 1,5-L-alpha-arabinobiose
-
-
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
very poor substrate
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
poor substrate
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
mild hydrolysis: no significant degradation, exhaustive enzymatic hydrolysis: extremely low degradation
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
DP3
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
poor substrate
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
poor substrate
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
DP4
composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times, product formation, intermediate enzymatic hydrolysis: L-arabinobiose and much less L-arabinose + L-arabinotriose, end-products after exhaustive enzymatic degradation
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
-
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
DP5
composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times, product formation, end-products after exhaustive enzymatic degradation, intermediate enzymatic hydrolysis: L-arabinobiose + L-arabinotriose
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
-
-
-
?
alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara-(1-5)-O-alpha-L-Ara + H2O
alpha-L-arabinofuranose + alpha-L-arabinofuranosyl-(1-5)-O-alpha-L-arabinofuranose
-
DP8
composition of end-products depends largely on substrate and enzyme concentrations, and on incubation times, product formation, end-products after exhaustive enzymatic degradation, intermediate enzymatic hydrolysis: L-arabinotriose + L-arabinopentaose and L-arabinobiose + L-arabinohexaose and L-arabinotetraose
?
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + H2O
L-arabinose
-
at very low rate, with high enzyme concentration
-
?
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + H2O
L-arabinose
-
at very low rate, with high enzyme concentration
-
?
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + H2O
L-arabinose
-
no or extremely low degradation
-
-
?
alpha-L-arabinopyranosyl-(1-5)-O-alpha-L-arabinopyranose + H2O
L-arabinose
-
DP2
-
-
?
arabinogalactan + H2O
?
-
for optimal breakdown of potato arabinogalactan combined action of endo-arabinase and endo-galactanase is required
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-
?