The enzyme, which was characterized from dormant seeds of the plant Cajanus cajan (pigeon pea), has been shown to remove the terminal non-reducing β-L-arabinopyranoside residue from the artificial substrate p-nitrophenyl-β-L-arabinopyranose . In the presence of methanol the enzyme demonstrates transglycosylase activity, transferring the arabinose moiety to methanol while retaining the anomeric configuration, generating 1-O-methyl-β-L-arabinopyranose .
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
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SYSTEMATIC NAME
IUBMB Comments
beta-L-arabinopyranoside non-reducing end beta-L-arabinopyranosidase
The enzyme, which was characterized from dormant seeds of the plant Cajanus cajan (pigeon pea), has been shown to remove the terminal non-reducing beta-L-arabinopyranoside residue from the artificial substrate p-nitrophenyl-beta-L-arabinopyranose [1]. In the presence of methanol the enzyme demonstrates transglycosylase activity, transferring the arabinose moiety to methanol while retaining the anomeric configuration, generating 1-O-methyl-beta-L-arabinopyranose [2].
Substrates: no activity with 4-nitrophenyl alpha-L-arabinofuranoside and arabino-oligosaccharide A-5b (composed of an alpha-1,5-linked trisaccharide replaced with a beta-1,3-linked arabinobiose side chain at position O-3) Products: -
assay described, activity measured in whole body homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity shown
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid)
the enzyme retains most of its activity at 65°C and 70°C for 1 h, but longer incubation times at all temperatures lead to drastic decreases of activity. After 10 min at 80°C, the enzyme shows at least 75% activity
comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major
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