Any feedback?
Information on EC 3.2.1.86 - 6-phospho-beta-glucosidase
for references in articles please use BRENDA:EC3.2.1.86Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
3.2.1.86 6-phospho-beta-glucosidaseIUBMB Comments
Also hydrolyses several other phospho-beta-D-glucosides, but not their non-phosphorylated forms.
Specify your search results
Word Map
- 3.2.1.86
-
phosphotransferase
- beta-glucoside
- arbutin
- salicin
-
phosphoenolpyruvate-dependent
-
antiterminator
-
beta-glucoside-specific
- mortiferum
- phospho-beta-galactosidase
- turanose
- palatinose
-
glycosylhydrolase
- trehalulose
- leucrose
- p-nitrophenyl-alpha-d-glucopyranoside
- maltulose
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
Synonyms
phospho-beta-glucosidase, 6-phospho-alpha-glucosidase, phospho-alpha-glucosidase, 6-phospho-beta-glucosidase, gan1d, lacg2, spy1599, lacg1, bgla-2, bgl-2, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
6-phospho-alpha-glucosidase
6-phospho-beta-glucosidase
Cellobiose-6-phosphate hydrolase
-
-
-
-
Gan1D
LacG1
LacG2
P-beta-glc
PalH
phospho-alpha-glucosidase
phospho-beta-glucosidase
phospho-beta-glucosidase A
phosphocellobiase
-
-
-
-
SPy1599
additional information
Gan1D
the enzyme exhibits bifunctional activity, possessing both 6-phospho-beta-galactosidase (primary function) and 6-phospho-beta-glucosidase activities
Gan1D
the enzyme exhibits bifunctional activity, possessing both 6-phospho-beta-galactosidase (primary function) and 6-phospho-beta-glucosidase activities
-
phospho-beta-glucosidase
-
-
-
-
phospho-beta-glucosidase A
-
-
-
-
additional information
the enzyme belongs to the glycsoylhydrolase family GH4. Glycosyl hydrolase family 4, GH4 is exceptional among the 114 families in this enzyme superfamily. Members of GH4 exhibit unusual cofactor requirements for activity, and an essential cysteine residue is present at the active site
additional information
the enzyme belongs to the glycsoylhydrolase family GH4. Glycosyl hydrolase family 4, GH4 is exceptional among the 114 families in this enzyme superfamily. Members of GH4 exhibit unusual cofactor requirements for activity, and an essential cysteine residue is present at the active site
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
6-phospho-beta-D-glucosyl-(1->4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate
-
-
-
-
6-phospho-beta-D-glucosyl-(1->4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate
active site structure and double displacement mechanism, overview
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
6-phospho-beta-D-glucosyl-(1->4)-D-glucose glucohydrolase
Also hydrolyses several other phospho-beta-D-glucosides, but not their non-phosphorylated forms.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
37205-51-9
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
2,4-dinitrophenol + D-glucose 6-phosphate
2,5-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
2,5-dinitrophenol + D-glucose 6-phosphate
-
-
-
-
?
2-nitrophenyl 6-phospho-beta-D-glucoside + H2O
2-nitrophenol + D-glucose 6-phosphate
-
-
-
-
?
2-nitrophenyl beta-D-galactoside 6-phosphate + H2O
2-nitrophenol + beta-D-galactose 6-phosphate
-
-
-
-
?
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
2-nitrophenol + beta-D-glucose 6-phosphate
3,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
3,4-dinitrophenol + D-glucose 6-phosphate
-
-
-
-
?
3,5-dichlorophenyl 6-phospho-beta-D-glucoside + H2O
3,5-dichlorophenol + D-glucose 6-phosphate
-
-
-
-
?
3-nitrophenyl 6-phospho-beta-D-glucoside + H2O
3-nitrophenol + D-glucose 6-phosphate
-
-
-
-
?
4-chloro-2-nitrophenyl 6-phospho-beta-D-glucoside + H2O
4-chloro-2-nitrophenol + D-glucose 6-phosphate
-
-
-
-
?
4-cyanophenyl 6-phospho-beta-D-glucoside + H2O
4-cyanophenol + D-glucose 6-phosphate
-
-
-
-
?
4-methyl-2-oxo-2H-chromen-7-yl 6-O-phosphono-beta-D-glucopyranoside + H2O
?
-
-
-
?
4-methylumbelliferyl-beta-D-glucopyranoside 6-phosphate + H2O
4-methylumbelliferone + D-glucose 6-phosphate
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucoside + H2O
4-methylumbelliferone + D-glucose
-
-
-
-
?
4-nitrophenyl 6-phospho-beta-D-glucoside + H2O
4-nitrophenol + D-glucose 6-phosphate
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
strains expressing the Bgl isoform Val204 show a lower hydrolysing activity on 4-nitrophenyl-beta-D-glucopyranoside when compared to strains that possess the Bgl isoform Ala204
-
-
?
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O
4-nitrophenol + beta-D-glucopyranose 6-phosphate
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O
4-nitrophenol + beta-D-glucose 6-phosphate
4-nitrothiophenyl beta-D-glucoside 6-phosphate + H2O
4-nitrothiophenol + beta-D-glucose 6-phosphate
-
-
-
-
?
4-tert-butylphenyl 6-phospho-beta-D-glucoside + H2O
4-tert-butylphenol + D-glucose 6-phosphate
-
-
-
-
?
cellobiose 6-phosphate + H2O
beta-D-glucose + glucose 6-phosphate
-
-
-
?
laminaribiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + D-galactose 6-phosphate
o-nitrophenyl-beta-D-galactose + H2O
o-nitrophenol + D-galactose
-
very low activity
-
-
?
p-nitrophenyl 6-phospho-beta-D-thioglucoside + H2O
p-nitrophenol + 6-phospho-beta-D-thioglucose
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
p-nitrophenol + D-glucose
-
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + beta-D-glucopyranose 6-phosphate
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucose 6-phosphate
-
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside-6-phosphate + H2O
p-nitrophenol + D-glucose 6-phosphate
-
-
-
-
?
p-nitrophenyl-beta-D-glucose + H2O
p-nitrophenol + D-glucose
-
very low activity
-
-
?
sophorose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
-
?
2,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
2,4-dinitrophenol + D-glucose 6-phosphate
-
high activity
-
-
?
2,4-dinitrophenyl 6-phospho-beta-D-glucoside + H2O
2,4-dinitrophenol + D-glucose 6-phosphate
-
-
-
-
?
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
2-nitrophenol + beta-D-glucose 6-phosphate
-
-
-
?
2-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
2-nitrophenol + beta-D-glucose 6-phosphate
-
-
-
?
4-nitrophenyl 6-phospho-beta-glucoside + H2O
4-nitrophenol + D-glucose 6-phosphate
-
-
-
-
?
4-nitrophenyl 6-phospho-beta-glucoside + H2O
4-nitrophenol + D-glucose 6-phosphate
-
-
-
?
4-nitrophenyl 6-phospho-beta-glucoside + H2O
4-nitrophenol + D-glucose 6-phosphate
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O
4-nitrophenol + beta-D-glucopyranose 6-phosphate
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O
4-nitrophenol + beta-D-glucopyranose 6-phosphate
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O
4-nitrophenol + beta-D-glucose 6-phosphate
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O
4-nitrophenol + beta-D-glucose 6-phosphate
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O
4-nitrophenol + beta-D-glucose 6-phosphate
-
-
-
?
6-phospho-beta-D-glucosyl-(1->4)-D-glucose + H2O
D-glucose + D-glucose 6-phosphate
-
-
-
?
6-phospho-beta-D-glucosyl-(1->4)-D-glucose + H2O
D-glucose + D-glucose 6-phosphate
-
-
-
-
?
arbutin 6-phosphate + H2O
hydroquinone + D-glucose 6-phosphate
-
-
-
-
?
arbutin 6-phosphate + H2O
hydroquinone + D-glucose 6-phosphate
-
-
-
?
arbutin 6-phosphate + H2O
hydroquinone + D-glucose 6-phosphate
-
-
-
-
?
arbutin 6-phosphate + H2O
hydroquinone + D-glucose 6-phosphate
-
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
?
gentiobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
?
gentiobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
-
?
gentiobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
-
?
lactose 6-phosphate + H2O
beta-D-galactose-6-phosphate + D-glucose
-
-
-
?
lactose 6-phosphate + H2O
beta-D-galactose-6-phosphate + D-glucose
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + D-galactose 6-phosphate
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + D-galactose 6-phosphate
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + D-galactose 6-phosphate
-
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
-
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
-
isoenzyme B
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
-
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + D-glucopyranose 6-phosphate
-
-
-
?
phenyl beta-D-glucoside 6-phosphate + H2O
phenol + glucose 6-phosphate
-
-
-
-
?
phenyl beta-D-glucoside 6-phosphate + H2O
phenol + glucose 6-phosphate
-
-
-
-
?
salicin 6-phosphate + H2O
2-hydroxymethylphenol + D-glucose 6-phosphate
-
-
-
?
salicin 6-phosphate + H2O
2-hydroxymethylphenol + D-glucose 6-phosphate
-
isoenzyme B
-
-
?
salicin 6-phosphate + H2O
2-hydroxymethylphenol + D-glucose 6-phosphate
-
-
-
-
?
salicin 6-phosphate + H2O
2-hydroxymethylphenol + D-glucose 6-phosphate
-
-
-
-
?
additional information
?
-
an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism
-
-
?
additional information
?
-
-
an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism
-
-
?
additional information
?
-
an essential cysteine residue is present at the active site. Members of GH4 employ a unique catalytic mechanism for cleavage of the glycosidic bond. GH4 single or double-displacement mechanism
-
-
?
additional information
?
-
-
expression of P-beta-glc induced by lactose is characteristic of Lactobacillus gasseri. Because this lactic acid bacterium is a major inhabitant of the human intestine, this enzyme is a key glycosidase involved in lactose utilization
-
-
?
additional information
?
-
the enzyme also shows 6-phospho-beta-galactosidase activity, EC 3.2.1.85
-
-
?
additional information
?
-
-
the enzyme also shows 6-phospho-beta-galactosidase activity, EC 3.2.1.85
-
-
?
additional information
?
-
the enzyme also shows 6-phospho-beta-galactosidase activity, EC 3.2.1.85
-
-
?
additional information
?
-
single nucleotide polymorphism in the bgl gene coding for the 6-phospho-beta-glucosidase is one possible cause of the difference in bacterial virulence among strains of Mycoplasma mycoides subsp. mycoides SC. Bgl does not act as a direct virulence factor, but strains possessing the Bgl isoform Val204 with low hydrolysing activity are more prone to survive in environments that contain high levels of beta-D-glucosides, thus contributing in some extent to mycoplasmaemia
-
-
?
additional information
?
-
-
the enzyme is not active against non-phosphorylated beta-glucosides
-
-
?
additional information
?
-
no activity with beta-(1,4)-thiocellobiose 6-phosphate
-
-
?
additional information
?
-
-
no activity with beta-(1,4)-thiocellobiose 6-phosphate
-
-
?
additional information
?
-
-
no activity with 2-nitrophenyl beta-D-galactopyranoside, 4-methylumbelliferyl N-acetyl-alpha-D-glucosaminide, 4-methylumbelliferyl beta-D-glucopyranoside, 4-methylumbelliferyl alpha-D-glucopyranoside, 2-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl alpha-D-glucoside 6-phosphate, 4-nitrophenyl alpha-D-mannoside 6-phosphate, and 4-nitrophenyl alpha-D-galactoside 6-phosphate
-
-
?
additional information
?
-
the enzyme SPy1599 contains a phosphate-binding pocket in the active site and has specific 6-phospho-beta-glucosidase activity. No or very poor activity with 4-nitrophenyl derivatives of galactose, glucose, xylose, galactose 6-phosphate, N-acetyl-D-galactosamine, and N-acetyl-D-glusosamine
-
-
?
additional information
?
-
-
the enzyme SPy1599 contains a phosphate-binding pocket in the active site and has specific 6-phospho-beta-glucosidase activity. No or very poor activity with 4-nitrophenyl derivatives of galactose, glucose, xylose, galactose 6-phosphate, N-acetyl-D-galactosamine, and N-acetyl-D-glusosamine
-
-
?
additional information
?
-
the enzyme SPy1599 contains a phosphate-binding pocket in the active site and has specific 6-phospho-beta-glucosidase activity. No or very poor activity with 4-nitrophenyl derivatives of galactose, glucose, xylose, galactose 6-phosphate, N-acetyl-D-galactosamine, and N-acetyl-D-glusosamine
-
-
?
additional information
?
-
the enzyme shows no beta-glucosidase activity, EC 3.2.1.21
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl 6-phospho-beta-glucoside + H2O
4-nitrophenol + D-glucose 6-phosphate
-
-
-
-
?
cellobiose 6-phosphate + H2O
beta-D-glucose + glucose 6-phosphate
-
-
-
?
6-phospho-beta-D-glucosyl-(1->4)-D-glucose + H2O
D-glucose + D-glucose 6-phosphate
-
-
-
?
6-phospho-beta-D-glucosyl-(1->4)-D-glucose + H2O
D-glucose + D-glucose 6-phosphate
-
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
?
cellobiose 6-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
-
-
?
additional information
?
-
-
expression of P-beta-glc induced by lactose is characteristic of Lactobacillus gasseri. Because this lactic acid bacterium is a major inhabitant of the human intestine, this enzyme is a key glycosidase involved in lactose utilization
-
-
?
additional information
?
-
single nucleotide polymorphism in the bgl gene coding for the 6-phospho-beta-glucosidase is one possible cause of the difference in bacterial virulence among strains of Mycoplasma mycoides subsp. mycoides SC. Bgl does not act as a direct virulence factor, but strains possessing the Bgl isoform Val204 with low hydrolysing activity are more prone to survive in environments that contain high levels of beta-D-glucosides, thus contributing in some extent to mycoplasmaemia
-
-
?
additional information
?
-
-
no activity with 2-nitrophenyl beta-D-galactopyranoside, 4-methylumbelliferyl N-acetyl-alpha-D-glucosaminide, 4-methylumbelliferyl beta-D-glucopyranoside, 4-methylumbelliferyl alpha-D-glucopyranoside, 2-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl alpha-D-glucoside 6-phosphate, 4-nitrophenyl alpha-D-mannoside 6-phosphate, and 4-nitrophenyl alpha-D-galactoside 6-phosphate
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
-
enzyme is completely inactive in the absence of NAD+. Kd-value is 480 nM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Mg2+
Mn2+
Ca2+
-
enhances activity, at 5 mM 11% increase in activity compared to activity without added ion
Mg2+
-
enhances activity, maximal hydrolysis of p-nitrophenyl-beta-D-glucopyranoside at 5 mM, 7% increase in activity compared to activity without added ion
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
no inhibition by cyclohexylammonium or cyclohexylamine salt
-
additional information
-
no inhibition by cyclohexylammonium or cyclohexylamine salt
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.3
4-methyl-2-oxo-2H-chromen-7-yl 6-O-phosphono-beta-D-glucopyranoside
-