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Information on EC 3.2.1.85 - 6-phospho-beta-galactosidase
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3.2.1.85 6-phospho-beta-galactosidaseSpecify your search results
Word Map
- 3.2.1.85
- lactose
-
phosphotransferase
- streptococcus
- lactobacillus
- lactococcus
- casei
-
phosphoenolpyruvate-dependent
- mutans
- gasseri
- tagatose
-
lactose-negative
-
leloir
-
lactose-specific
-
iilac
- beta-galactosides
- lacr
- phospho-beta-glucosidase
- cremoris
- sanguis
- diagnostics
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
phospho-beta-galactosidase, p-beta-gal, 6-phospho-beta-galactosidase, beta-d-phosphogalactoside galactohydrolase, 6-phospho-beta-d-galactosidase, beta-pgal, p-beta-galactosidase, bgase-iii, beta-phosphogalactosidase, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
beta-D-phosphogalactoside galactohydrolase
BGase-III
Gan1D
LacG1
LacG2
P-beta-gal
P-bgal
PBG
-
-
-
-
phospho-beta-galactosidase
Gan1D
the enzyme exhibits bifunctional activity, possessing both 6-phospho-beta-galactosidase (primary function) and 6-phospho-beta-glucosidase activities
Gan1D
the enzyme exhibits bifunctional activity, possessing both 6-phospho-beta-galactosidase (primary function) and 6-phospho-beta-glucosidase activities
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
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SYSTEMATIC NAME
IUBMB Comments
6-phospho-beta-D-galactoside 6-phosphogalactohydrolase
-
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CAS REGISTRY NUMBER
COMMENTARY
37237-42-6
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-nitrophenyl beta-D-galactopyranoside 6-phosphate + H2O
2-nitrophenyl beta-D-galactopyranose + phosphate
-
-
-
-
?
2-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
2-nitrophenol + beta-D-galactose 6-phosphate
4-nitrophenyl 6-phospho-beta-D-galactopyranoside + H2O
4-nitrophenol + 6-phospho-beta-D-galactopyranose
-
-
-
-
?
4-nitrophenyl 6-phospho-beta-D-glucopyranoside + H2O
4-nitrophenol + 6-phospho-beta-D-glucopyranose
-
-
-
-
?
lactose 6-phosphate + H2O
D-glucose + D-galactose 6-phosphate
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose 6-phosphate
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
o-nitrophenyl-beta-D-galactoside + H2O
o-nitrophenol + beta-D-galactose
-
-
-
?
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
o-nitrophenol + beta-D-glucose-6-phosphate
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-glucose-6-phosphate
-
-
-
?
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
p-nitrophenol + beta-D-galactose-6-phosphate
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
p-nitrophenol + beta-D-glucose-6-phosphate
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + beta-D-glucose-6-phosphate
less effective than ortho-form
-
-
?
2-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
2-nitrophenol + beta-D-galactose 6-phosphate
-
-
-
?
2-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
2-nitrophenol + beta-D-galactose 6-phosphate
-
-
-
?
cellobiose-6-phosphate + H2O
D-glucose-6-phosphate + D-glucose
-
-
-
?
cellobiose-6-phosphate + H2O
D-glucose-6-phosphate + D-glucose
-
-
-
?
lactose + H2O
D-glucose + D-galactose
-
existence of an alternative lactose catabolic pathway in the enzyme-deficient mutant strain IL1403 by phospho-beta-galactosidase activity exhibited by BglS, a phospho-beta-glucosidase, the lactose hydrolysis is catalyzed by both phospho-beta-galactosidase and phospho-beta-glucosidase, the latter being the main enzyme in the pathway
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
involved in lactose assimilation
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
o-nitrophenol + beta-D-glucose-6-phosphate
-
only little activity
-
?
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
o-nitrophenol + beta-D-glucose-6-phosphate
-
only little activity
-
?
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
o-nitrophenol + beta-D-glucose-6-phosphate
-
only little activity
-
?
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
p-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
p-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
p-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
p-nitrophenol + beta-D-galactose-6-phosphate
less effective than ortho-form
-
-
?
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
p-nitrophenol + beta-D-galactose-6-phosphate
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
p-nitrophenol + beta-D-glucose-6-phosphate
-
only little activity
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
p-nitrophenol + beta-D-glucose-6-phosphate
-
only little activity
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
p-nitrophenol + beta-D-glucose-6-phosphate
-
only little activity
-
?
additional information
?
-
the enzyme also shows 6-phospho-beta-glucosidase activity, EC 3.2.1.86
-
-
?
additional information
?
-
the enzyme also shows 6-phospho-beta-glucosidase activity, EC 3.2.1.86
-
-
?
additional information
?
-
-
physiologic regulation of the pathway, overview
-
-
?
additional information
?
-
-
Pyrococcus furiosus beta-glucosidase has been mutated to increase its activity toward galactosides by incorporating residues present in Lactococcus lactis 6-phospho-beta-galactosidase
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
lactose + H2O
D-glucose + D-galactose
-
existence of an alternative lactose catabolic pathway in the enzyme-deficient mutant strain IL1403 by phospho-beta-galactosidase activity exhibited by BglS, a phospho-beta-glucosidase, the lactose hydrolysis is catalyzed by both phospho-beta-galactosidase and phospho-beta-glucosidase, the latter being the main enzyme in the pathway
-
-
?
additional information
?
-
-
physiologic regulation of the pathway, overview
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
involved in lactose assimilation
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
-
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Cu2+
Hg2+
Mg2+
Mn2+
Zn2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2,4-dinitrophenyl-2-deoxy-2-bromo-beta-D-galactopyranosyl-6-phosphate
-
fluoro-, chloro- and bromo-derivatives, complete inactivation, most rapidly with fluoro-, followed by chloro- and bromo-derivatives
2,4-dinitrophenyl-2-deoxy-2-chloro-beta-D-galactopyranosyl-6-phosphate
-
fluoro-, chloro- and bromo-derivatives, complete inactivation, most rapidly with fluoro-, followed by chloro- and bromo-derivatives
2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-galactopyranosyl-6-phosphate
-
fluoro-, chloro- and bromo-derivatives, complete inactivation, most rapidly with fluoro-, followed by chloro- and bromo-derivatives
p-hydroxymercuribenzoate
-
83.4% of control activity at 1 mM, 12.2% at 2 mM
p-nitrophenyl-beta-thiogalactoside 6-phosphate
-
competitive inhibition
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
dithiothreitol
-
enhanced activiy after dialysis by addition up to 0.75 mM
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Infections
T7 infection-dependent selective expression of cloned genes in P1-lysogenic Escherichia coli.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3.8
2-nitrophenyl-beta-D-galactopyranoside 6-phosphate
pH 6.0, 40°C
5.5
2-nitrophenyl-beta-D-galactopyranoside 6-phosphate
pH 5.5, 50°C
0.54
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
wild type enzyme, pH 7.5, room temperature
0.6 - 3
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
0.6 - 3
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
2.0 hybrid with protein A
0.76 - 0.8
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
1.3 - 1.59
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
2.86
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
W429A mutant enzyme, pH 7.5, room temperature
4.89
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
K435V/Y437F mutant enzyme, pH 7.5, room temperature
7.3
o-nitrophenyl-beta-D-galactopyranoside