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Information on EC 3.2.1.80 - fructan beta-fructosidase and Organism(s) Aspergillus awamori and UniProt Accession Q96TU3

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EC Tree
IUBMB Comments
Hydrolyses inulin and levan, and also sucrose.
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This record set is specific for:
Aspergillus awamori
UNIPROT: Q96TU3
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Word Map
The taxonomic range for the selected organisms is: Aspergillus awamori
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
glycoside hydrolase, 1-feh, exoinulinase, fructanase, fructan exohydrolase, 6-feh, fructan 1-exohydrolase, 1-feh iia, 1-feh i, wfh-sm3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2,1-beta-D-fructan fructohydrolase
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exo-beta-D-fructosidase
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exo-beta-fructosidase
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-
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fructan exohydrolase
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Fructanase
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fructofuranosidase, polysaccharide beta
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-
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polysaccharide beta-fructofuranosidase
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
beta-D-fructan fructohydrolase
Hydrolyses inulin and levan, and also sucrose.
CAS REGISTRY NUMBER
COMMENTARY hide
37288-56-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram
-
-
-
?
inulobiose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram
inulin is an intermediate product
-
-
?
inuloheptaose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram
inulohexaose, inulopentaose, inulotetraose, inulotriose, inulobiose and inulin are intermediate products
-
-
?
inulohexaose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram
inulopentaose, inulotetraose, inulotriose, inulobiose and inulin are intermediate products
-
-
?
inulopentaose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram
inulotetraose, inulotriose, inulobiose and inulin are intermediate products
-
-
?
inulotetraose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram
inulotriose, inulobiose and inulin are intermediate products
-
-
?
inulotriose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram
inulobiose and inulin are intermediate products
-
-
?
levan + H2O
beta-D-fructofuranose + alpha-D-glucopyranose + ?
show the reaction diagram
-
-
-
?
levanohexaose + 6 H2O
6 beta-D-fructofuranose
show the reaction diagram
levanopentaose, levanotetraose, levanotriose, levanobiose and levan are intermediate products
-
-
?
levanopentaose + 4 H2O
5 beta-D-fructofuranose
show the reaction diagram
levanotetraose, levanotriose, levanobiose and levan are intermediate products
-
-
?
levanotetraose + 3 H2O
4 beta-D-fructofuranose
show the reaction diagram
levanotriose, levanobiose and levan are intermediate products
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-
?
levanotriose + 2 H2O
3 beta-D-fructofuranose
show the reaction diagram
levanobiose and levan are intermediate products
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-
?
sucrose + H2O
beta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram
-
-
-
?
inulin + H2O
beta-D-fructose + ?
show the reaction diagram
-
-
-
-
?
levan + H2O
beta-D-fructose + ?
show the reaction diagram
-
-
-
-
?
sucrose + H2O
beta-D-fructose + D-glucose
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
inulin + 5 H2O
5 beta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
inulin
at 37ºC, pH 4.5
3.72
Inulobiose
at 37ºC, pH 4.5
1.1
inuloheptaose
at 37ºC, pH 4.5
1.02
inulohexaose
at 37ºC, pH 4.5
1.08
inulopentaose
at 37ºC, pH 4.5
0.94
inulotetraose
at 37ºC, pH 4.5
1.63
inulotriose
at 37ºC, pH 4.5
1.01
levanohexaose
at 37ºC, pH 4.5
10.2
levanopentaose
at 37ºC, pH 4.5
2.74
levanotetraose
at 37ºC, pH 4.5
0.21
levanotriose
at 37ºC, pH 4.5
40
sucrose
at 37ºC, pH 4.5
additional information
additional information
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
var. 2250
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
INUE_ASPAW
537
0
59171
Swiss-Prot
Secretory Pathway (Reliability: 2)
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
no modification
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glycoprotein
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme-fructose-complex, 1.87 A resolution, single isomorphous replacement, 20% fructose solution
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free enzyme, 1.5 A resolution, single isomorphous replacement, orthorhombic crystals, 0.5 M sodium iodide and flash-cooling to -173°C in stream of cold nitrogen
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nutrition
microbial production of pure beta-D-fructofuranose or fructose syrup
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kulminskaya, A.A.; Arand, M.; Eneyskaya, E.V.; Ivanen, D.R.; Shabalin, K.A.; Shishlyannikov, S.M.; Saveliev, A.N.; Korneeva, O.S.; Neustroev, K.N.
Biochemical characterization of Aspergillus awamori exoinulinase: substrate binding characteristics and regioselectivity of hydrolysis
Biochim. Biophys. Acta
1650
22-29
2003
Aspergillus awamori (Q96TU3), Aspergillus awamori
Manually annotated by BRENDA team
Nagem, R.A.; Rojas, A.L.; Golubev, A.M.; Korneeva, O.S.; Eneyskaya, E.V.; Kulminskaya, A.A.; Neustroev, K.N.; Polikarpov, I.
Crystal structure of exo-inulinase from Aspergillus awamori: the enzyme fold and structural determinants of substrate recognition
J. Mol. Biol.
344
471-480
2004
Aspergillus awamori, Aspergillus awamori EI
Manually annotated by BRENDA team