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Information on EC 3.2.1.8 - endo-1,4-beta-xylanase and Organism(s) Pseudoalteromonas haloplanktis and UniProt Accession Q8RJN8

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Pseudoalteromonas haloplanktis
UNIPROT: Q8RJN8 not found.
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The taxonomic range for the selected organisms is: Pseudoalteromonas haloplanktis
The enzyme appears in selected viruses and cellular organisms
Synonyms
endoxylanase, xylanase a, endo-xylanase, xyn11a, xyn10a, beta-xylanase, endo-1,4-beta-xylanase, gh11 xylanase, xylanase b, xynii, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(1--> 4)-beta-xylan 4-xylanohydrolase
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-
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1,4-beta-D-xylan xylanohydrolase
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1,4-beta-D-xylan xylanohydrolase 22
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1,4-beta-xylan xylanohydrolase
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34 kDa xylanase
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beta-1,4-D-xylanase
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beta-1,4-xylan xylanohydrolase
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beta-1,4-xylanase
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beta-D-xylanase
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beta-xylanase
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endo-(1--> 4)-beta-xylanase
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endo-1,4-beta-D-xylanase
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endo-1,4-beta-xylanase
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endo-1,4-xylanase
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endo-beta-1,4-xylanase
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endoxylanase
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FIA-xylanase
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ORF4
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TAXI
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X34
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XYLA
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xylanase
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Xylanase 22
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xylanase, endo-1,4-
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XYLD
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XYLY
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additional information
enzyme belongs to family 8 of glycosyl hydrolases
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
show the reaction diagram
PATHWAY SOURCE
PATHWAYS
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-, -
SYSTEMATIC NAME
IUBMB Comments
4-beta-D-xylan xylanohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
9025-57-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
xylan + H2O
D-xylotriose + xylotetraose + ?
show the reaction diagram
xylooligosaccharide + H2O
D-xylotriose + xylotetraose
show the reaction diagram
long-chain substrates, high activity
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-
?
xylopentaose + H2O
xylotriose + xylobiose
show the reaction diagram
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-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
xylan + H2O
D-xylotriose + xylotetraose + ?
show the reaction diagram
major constituent of plant hemicellulose forming the cell walls of hards woods, 15-30%, and softwoods, 7-10%
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?
additional information
?
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unrooted evolutionary tree, overview
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-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
16% inhibition at 1 mM
Hg2+
31% inhibition at 1 mM
Ni2+
6% inhibition at 1 mM
Zn2+
14% inhibition at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
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-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1247
xylan
pH 6.5, 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
psychrophilic enzyme with high activity at low temperatures and low thermal stability
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 35
60% of maximal activity at 5°C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q8RJN8_PSEHA
426
0
48407
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45982
x * 45982, mass spectrometry
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 45982, mass spectrometry
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
mutants D144A, E78Q, in complex with substrate xylopentaose and product xylotriose
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D144A
crystallization data
E78Q
crystallization data
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
psychrophilic enzyme with high activity at low temperatures and low thermal stability
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme from Escherichia coli, native enzyme 11fold by ultrafiltration and ion exchange chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli BL21(DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Collins, T.; Meuwis, M.A.; Stals, I.; Claeyssens, M.; Feller, G.; Gerday, C.
A novel family 8 xylanase, functional and physicochemical characterization
J. Biol. Chem.
277
35133-35139
2002
Pseudoalteromonas haloplanktis (Q8RJN8)
Manually annotated by BRENDA team
De Vos, D.; Collins, T.; Nerinckx, W.; Savvides, S.N.; Claeyssens, M.; Gerday, C.; Feller, G.; Van Beeumen, J.
Oligosaccharide binding in family 8 glycosidases: crystal structures of active-site mutants of the beta-1,4-xylanase pXyl from Pseudoaltermonas haloplanktis TAH3a in complex with substrate and product
Biochemistry
45
4797-4807
2006
Pseudoalteromonas haloplanktis (Q8RJN8), Pseudoalteromonas haloplanktis TAH3a (Q8RJN8), Pseudoalteromonas haloplanktis TAH3a
Manually annotated by BRENDA team