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Information on EC 3.2.1.8 - endo-1,4-beta-xylanase and Organism(s) Neocallimastix patriciarum and UniProt Accession P29127
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3.2.1.8 endo-1,4-beta-xylanaseSpecify your search results
Word Map
- 3.2.1.8
- xylans
- xylanases
- xylose
- arabinoxylans
- trichoderma
- xylooligosaccharides
- reesei
- xylotriose
-
xylanolytic
-
birchwood
- spelt
- cellulases
- straw
-
carbohydrate-binding
-
lignocellulosic
- beta-xylosidase
- xylotetraose
- bran
-
hemicellulases
- lanuginosus
- thermomyces
- corncob
- arabinofuranosidase
- bagasse
- cellobiohydrolase
- alpha-l-arabinofuranosidase
- cellulomonas
-
saccharification
-
cellulose-binding
- avicel
-
thermoascus
- degradation
- agriculture
- cellulosome
- paper production
- glucuronoxylans
-
lignocellulolytic
- endo-1,4-beta-glucanase
- halodurans
- arabinan
- industry
- xylosidase
- biofuel production
-
breadmaking
- analysis
-
hemicellulolytic
- nutrition
- cellulolyticus
- longibrachiatum
-
taxi
- food industry
- synthesis
-
kraft
- biotechnology
-
biobleaching
-
cellulase-free
- neocallimastix
-
water-extractable
- cellulosa
-
dockerin
The taxonomic range for the selected organisms is: Neocallimastix patriciarum
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
endoxylanase, xylanase a, endo-xylanase, xyn11a, xyn10a, beta-xylanase, endo-1,4-beta-xylanase, gh11 xylanase, xylanase b, xynii, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
(1--> 4)-beta-xylan 4-xylanohydrolase
-
-
-
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1,4-beta-D-xylan xylanohydrolase
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-
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1,4-beta-D-xylan xylanohydrolase 22
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1,4-beta-xylan xylanohydrolase
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-
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34 kDa xylanase
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-
-
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beta-1,4-D-xylanase
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-
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beta-1,4-xylan xylanohydrolase
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-
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beta-1,4-xylanase
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-
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beta-D-xylanase
-
-
-
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beta-xylanase
-
-
-
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endo-(1--> 4)-beta-xylanase
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-
-
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endo-1,4-beta-D-xylanase
-
-
-
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endo-1,4-beta-xylanase
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-
-
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endo-1,4-xylanase
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-
-
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endo-beta-1,4-xylanase
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-
-
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endoxylanase
FIA-xylanase
-
-
-
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ORF4
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-
-
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TAXI
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-
-
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X34
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-
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XYLA
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-
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xylanase
Xylanase 22
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xylanase, endo-1,4-
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XYLD
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XYLY
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endoxylanase
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xylanase
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SYSTEMATIC NAME
IUBMB Comments
4-beta-D-xylan xylanohydrolase
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CAS REGISTRY NUMBER
COMMENTARY
9025-57-4
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-glucosyl-(1->4)-[(1->4)-beta-D-xylopentaoside] + H2O
?
-
-
-
?
beechwood xylan + H2O
?
161% of the activity with xyloheptaose
-
-
?
wheat arabinoxylan + H2O
?
117% of the activity with xyloheptaose
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-
?
arabinoxylan + H2O
?
-
GH 10 enzyme produces smaller oligosaccharides than GH 11. GH 11 produces oligosaccharides with unsubstituted xylopyranose at the reducing- and non-reducing ends
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-
?
xylohexaose + H2O
?
98% of the activity with xyloheptaose
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-
?
xylopentaose + H2O
?
49% of the activity with xyloheptaose
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-
?
xylotetraose + H2O
?
1% of the activity with xyloheptaose
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-
?
additional information
?
-
no activity against carboxy methyl cellulose, beta-glucan, lichenan, laminarin, agarose, starch, and kappa-carageenan
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-
?
additional information
?
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no activity against carboxy methyl cellulose, beta-glucan, lichenan, laminarin, agarose, starch, and kappa-carageenan
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-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
in the insoluble fraction of cell lysate or embedding in artificial oil bodies, XynS20-intein M-oleosin does not show any enzymatic activity. After release from artificial oil bodies by inclusion of DTT to induce intein linker self-splicing, XynS20 reveals a xylanase activity band of about 36 kDa
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additional information
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in the insoluble fraction of cell lysate or embedding in artificial oil bodies, XynS20-intein M-oleosin does not show any enzymatic activity. After release from artificial oil bodies by inclusion of DTT to induce intein linker self-splicing, XynS20 reveals a xylanase activity band of about 36 kDa
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.166
4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-glucosyl-(1->4)-[(1->4)-beta-D-xylopentaoside]
pH 6.0, 50°C
15
4-nitrophenyl-beta-D-xylobioside
in 12 mM sodium citrate, 50 mM sodium phosphate buffer (pH 6.5)
2.1
4-nitrophenyl-beta-D-xylotrioside
in 12 mM sodium citrate, 50 mM sodium phosphate buffer (pH 6.5)
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). XYNA_NEOPA
607
0
66175
Swiss-Prot
Secretory Pathway (Reliability: 3)
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
x * 25827, mutant N38Y/F52W/G56Y/G201L, calculated, x * 25800, SDS-PAGE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme bound to ferulic acid-1,5-arabinofuranose-alpha-1,3-xylotriose, hanging drop vapour diffusion method, with 0.1 M cadmium chloride, 0.1 M sodium acetate (pH 4.6) and 30% (w/v) polyethylene glycol 400
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
N38Y/F52W/G56Y/G201L
increase in melting temperature by 14 degrees
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4 - 7
when pH is below 4.0 or above 7.0, less than 60% of the optimal activity is retained and the enzyme shows no activity at pH 9.0
695791
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30 - 55
at temperatures below 30°C and above 55°C, enzyme activity is less than 50% of that at the optimal temperature
65
20 min, wild-type, 70% loss of activity, mutant N38Y/F52W/G56Y/G201L, about 10% loss of activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA fragments of xynS20 genes subcloned into vector pOSP2. XynS20 expressed as a recombinant protein fused to the N-terminus of oleosin by a linker polypeptide, intein M, in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
enzyme coupled assay procedure, using substrate 4-nitrophenyl 4,6-O-(3-oxobutylidene)-beta-D-glucosyl-(1->4)-[(1->4)-beta-D-xylopentaoside] and release of 4-nitrophenol by beta-xylosidase
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Berrin, J.G.; Juge, N.
Factors affecting xylanase functionality in the degradation of arabinoxylans
Biotechnol. Lett.
30
1139-150
2008
Bacillus sp. (in: Bacteria), Cellvibrio japonicus, Cellvibrio japonicus (Q59675), Acetivibrio thermocellus (O52780), Streptomyces lividans (P26514), Neocallimastix patriciarum (P29127), Paenibacillus polymyxa (P45796), Cellulomonas fimi (P54865), Aspergillus niger (P55329), Rhodothermus marinus (P96988), Aspergillus nidulans (Q00177), Thermotoga maritima (Q60037), Streptomyces olivaceoviridis (Q7SI98), Thermoclostridium stercorarium (Q8GJ44), Talaromyces funiculosus (Q9HFH0), Trichoderma viride (Q9UVF9)
Maslen, S.L.; Goubet, F.; Adam, A.; Dupree, P.; Stephens, E.
Structure elucidation of arabinoxylan isomers by normal phase HPLC-MALDI-TOF/TOF-MS/MS
Carbohydr. Res.
342
724-735
2007
Cellvibrio japonicus, Neocallimastix patriciarum
Vardakou, M.; Dumon, C.; Murray, J.W.; Christakopoulos, P.; Weiner, D.P.; Juge, N.; Lewis, R.J.; Gilbert, H.J.; Flint, J.E.
Understanding the structural basis for substrate and inhibitor recognition in eukaryotic GH11 xylanases
J. Mol. Biol.
375
1293-1305
2008
Neocallimastix patriciarum (P29127), Neocallimastix patriciarum
Liu, J.R.; Duan, C.H.; Zhao, X.; Tzen, J.T.; Cheng, K.J.; Pai, C.K.
Cloning of a rumen fungal xylanase gene and purification of the recombinant enzyme via artificial oil bodies
Appl. Microbiol. Biotechnol.
79
225-233
2008
Neocallimastix patriciarum (A8TGA1), Neocallimastix patriciarum
Bu, Y.; Cui, Y.; Peng, Y.; Hu, M.; Tian, Y.; Tao, Y.; Wu, B.
Engineering improved thermostability of the GH11 xylanase from Neocallimastix patriciarum via computational library design
Appl. Microbiol. Biotechnol.
102
3675-3685
2018
Neocallimastix patriciarum (Q9UV68), Neocallimastix patriciarum
Mangan, D.; Cornaggia, C.; Liadova, A.; McCormack, N.; Ivory, R.; McKie, V.A.; Ormerod, A.; McCleary, B.V.
Novel substrates for the automated and manual assay of endo-1,4-beta-xylanase
Carbohydr. Res.
445
14-22
2017
Cellvibrio mixtus (O68541), Neocallimastix patriciarum (P29127), Aspergillus niger (P55329)
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