Information on EC 3.2.1.72 - xylan 1,3-beta-xylosidase

for references in articles please use BRENDA:EC3.2.1.72
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

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EC NUMBER
COMMENTARY hide
3.2.1.72
-
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RECOMMENDED NAME
GeneOntology No.
xylan 1,3-beta-xylosidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of successive xylose residues from the non-reducing termini of (1->3)-beta-D-xylans
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
(1,3)-beta-D-xylan degradation
-
-
d-xylose degradation
-
-
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SYSTEMATIC NAME
IUBMB Comments
3-beta-D-xylan xylohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
37288-50-9
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Swissprot
Manually annotated by BRENDA team
-
Swissprot
Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-naphthylacetate + H2O
alpha-naphthol + acetate
show the reaction diagram
-
-
-
-
?
beta -1,4-xylotetraose
D-xylose
show the reaction diagram
beta-1,3-xylobiose + H2O
D-xylose
show the reaction diagram
beta-1,3-xylotetraose + H2O
D-xylose
show the reaction diagram
beta-1,3-xylotriose + H2O
D-xylose
show the reaction diagram
beta-1,4-xylobiose
D-xylose
show the reaction diagram
low activity
-
-
-
beta-1,4-xylotriose
D-xylose
show the reaction diagram
p-nitrophenyl-beta-D-xylopyranose + H2O
p-nitrophenol + D-xylopyranose
show the reaction diagram
no activity on laminarioligosaccharides, beta-1,3-linked D-glucose units, beta-1,3-xylan, beta-1,4-xylan, carboxymethyl cellulose, laminaran, p-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-D-mannopyranoside, p-nitrophenyl-beta-D-mannopyranoside, p-nitrophenyl-beta-Dgalactopyranoside, p-nitrophenyl-beta-D-glucopyranoside, p-nitrophenyl-beta-D-glucopyranoside, p-nitrophenyl-beta-L-fucopyranoside, p-nitrophenyl-beta-L-arabinofuranoside, p-nitrophenyl-beta-L-arabinopyranoside, p-nitrophenyl-beta-D-glucuronide, and p-nitrophenyl-beta-D-cellobioside
-
-
?
beta-1,3-xylan + H2O
additional information
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
beta-1,3-xylan + H2O
additional information
-
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
more than 90% inhibition
Cu2+
more than 90% inhibition
Hg2+
more than 90% inhibition
Mn2+
more than 90% inhibition
p-chloromercuribenzoic acid
more than 90% inhibition
Pb2+
more than 90% inhibition
Zn2+
more than 90% inhibition
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.244
p-nitrophenyl-beta-D-xylopyranoside
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
with 2 mM p-nitrophenyl-beta-D-xylopyranoside at 37°C
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
more than 90% of maximum activity
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60000
SDS-PAGE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9
-
stable for 30 min
742766
6 - 9
retaining more than 80% of the original activity after preincubation in the buffer at various pHs at 4°C for 12 h
677708
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
30 min, stable up to
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
wild type by DEAE-Toyopearl 650M and MonoQ 5/50 column chromatography, recombinant protein by HiTrap chelating HP column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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LINKS TO OTHER DATABASES (specific for EC-Number 3.2.1.72)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)