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Information on EC 3.2.1.6 - endo-1,3(4)-beta-glucanase

for references in articles please use BRENDA:EC3.2.1.6
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EC Tree
IUBMB Comments
Substrates include laminarin, lichenin and cereal D-glucans; different from EC 3.2.1.52 beta-N-acetylhexosaminidase.
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This record set is specific for:
UNIPROT: Q000P7
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Word Map
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
cmcase, lichenase, endo-beta-1,4-glucanase, endo-1,4-beta-glucanase, beta-1,3-1,4-glucanase, cel8a, h(a16-m), lam16a, fsbeta-glucanase, endo-beta-1,3-1,4-glucanase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
endo-1,3-beta-glucanase
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3(or 4)-beta-D-glucan 3(4)-glucanohydrolase
Substrates include laminarin, lichenin and cereal D-glucans; different from EC 3.2.1.52 beta-N-acetylhexosaminidase.
CAS REGISTRY NUMBER
COMMENTARY hide
62213-14-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
barley beta-glucan + H2O
?
show the reaction diagram
-
-
-
?
curdlan + H2O
?
show the reaction diagram
from Alcaligenes faecalis
-
-
?
laminarin + H2O
laminaritriose
show the reaction diagram
from Laminaria digitata
-
-
?
lichenan + H2O
?
show the reaction diagram
-
-
-
?
pachyman + H2O
?
show the reaction diagram
-
-
-
?
zymosan A + H2O
?
show the reaction diagram
from Saccharomyces cerevisiae
-
-
?
additional information
?
-
no activity with carboxymethylcellulose and xylan of all truncated proteins of LamA
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
Laminarin
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
toward laminarin hydrolysis for truncated proteins CBF and C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
for truncated protein CBF, pH 5.5
50
for truncated protein C, pH 5.5
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q000P7_9BACL
1792
0
190148
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
144600
calculated molecular mass of expressed CBF of LamA
38200
calculated molecular mass of expressed C of LamA
51600
calculated molecular mass of expressed CF of LamA
77000
calculated molecular mass of expressed SC of LamA
92000
calculated molecular mass of expressed CB3 of LamA
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
immobilized Ni2+ affinity chromatography, gel filtration chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of five truncated proteins of LamA in Escherichia coli BL21(DE3)
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
the various truncated proteins of LamA also show inhibition on the mycelium growth of Rhizopus solani, a pathogen infecting a broad range of many crops, suggesting the application potential of LamA as an antifungal agent in many aspects
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cheng, Y.M.; Hong, T.Y.; Liu, C.C.; Meng, M.
Cloning and functional characterization of a complex endo-beta-1,3-glucanase from Paenibacillus sp.
Appl. Microbiol. Biotechnol.
81
1051-1061
2009
Paenibacillus sp. CCRC 17245 (Q000P7)
Manually annotated by BRENDA team