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Information on EC 3.2.1.55 - non-reducing end alpha-L-arabinofuranosidase and Organism(s) Bifidobacterium longum and UniProt Accession Q841V6

for references in articles please use BRENDA:EC3.2.1.55
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EC Tree
IUBMB Comments
The enzyme acts on alpha-L-arabinofuranosides, alpha-L-arabinans containing (1,3)- and/or (1,5)-linkages, arabinoxylans and arabinogalactans. Some beta-galactosidases (EC 3.2.1.23) and beta-D-fucosidases (EC 3.2.1.38) also hydrolyse alpha-L-arabinosides. cf. EC 3.2.1.185, non-reducing end beta-L-arabinofuranosidase.
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This record set is specific for:
Bifidobacterium longum
UNIPROT: Q841V6
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Word Map
The taxonomic range for the selected organisms is: Bifidobacterium longum
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
arabinofuranosidase, arabinosidase, alpha-l-araf, alpha-arabinosidase, alpha-l-arabinosidase, afase, alpha-l-arabinofuranosidase b, arabinoxylan arabinofuranohydrolase, alpha-araf, alpha-arabinofuranosidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha-L-arabinofuranosidase
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ABF
-
-
-
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alpha-arabinofuranosidase
-
-
-
-
alpha-arabinosidase
-
-
-
-
Alpha-L-AF
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-
-
-
alpha-L-Afase
-
-
alpha-L-arabinanase
-
-
-
-
alpha-L-arabinofuranosidase
alpha-L-arabinofuranoside arabinofuranohydrolase
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alpha-L-arabinofuranoside hydrolase
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-
-
-
alpha-L-arabinosidase
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-
-
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arabinosidase
-
-
-
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L-arabinosidase
-
-
-
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polysaccharide alpha-L-arabinofuranosidase
-
-
-
-
additional information
enzyme belongs to the family 51 of glycosidases
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
show the reaction diagram
exo-acting enzyme
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase
The enzyme acts on alpha-L-arabinofuranosides, alpha-L-arabinans containing (1,3)- and/or (1,5)-linkages, arabinoxylans and arabinogalactans. Some beta-galactosidases (EC 3.2.1.23) and beta-D-fucosidases (EC 3.2.1.38) also hydrolyse alpha-L-arabinosides. cf. EC 3.2.1.185, non-reducing end beta-L-arabinofuranosidase.
CAS REGISTRY NUMBER
COMMENTARY hide
9067-74-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
show the reaction diagram
-
-
-
?
alpha-(1->5)-L-arabinofuranotriose + H2O
alpha-L-arabinofuranose
show the reaction diagram
-
-
-
?
alpha-L-arabinofuranobiose + H2O
alpha-L-arabinofuranose
show the reaction diagram
-
-
-
?
alpha-L-arabinofuranopentaose + H2O
alpha-L-arabinofuranose
show the reaction diagram
-
-
-
?
alpha-L-arabinofuranoptetraose + H2O
alpha-L-arabinofuranose
show the reaction diagram
-
-
-
?
arabinan + H2O
alpha-L-arabinose + ?
show the reaction diagram
-
-
-
?
arabinoxylan + H2O
alpha-L-arabinose + ?
show the reaction diagram
-
-
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
show the reaction diagram
100% activity
-
-
?
arabinan + H2O
L-arabinose + ?
show the reaction diagram
-
-
-
-
?
arabinoxylan + H2O
L-arabinose + ?
show the reaction diagram
-
-
-
-
?
ginsenoside Rc + H2O
ginsenoside Rd + L-arabinofuranose
show the reaction diagram
10.5% activity compared to 4-nitrophenyl alpha-L-arabinofuranoside
-
-
?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
109.4% relative activity at 1 mM
Na+
the enzyme activity is unaffected by NaCl concentration in the range of 0-1 mM
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ba2+
68% residual activity at 1 mM
Co2+
54.4% residual activity at 1 mM
Cu2+
7% residual activity at 1 mM
Fe2+
81.3% residual activity at 1 mM
Mg2+
80.2% residual activity at 1 mM
Ni2+
85.1% residual activity at 1 mM
additional information
not affected by chelating or reducing agents
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.295
4-nitrophenyl-alpha-L-arabinofuranoside
pH 6.0, 45°C
0.21
4-nitrophenyl alpha-L-arabinofuranoside
in 50 mM sodium acetate buffer (pH 5.5), at 37°C
1.73
ginsenoside Rc
in 50 mM sodium acetate buffer (pH 5.5), at 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.64
crude extract, using 4-nitrophenyl alpha-L-arabinofuranoside as substrate, in 50 mM sodium acetate buffer (pH 5.5), at 37°C
71.36
after 26fold purification, using 4-nitrophenyl alpha-L-arabinofuranoside as substrate, in 50 mM sodium acetate buffer (pH 5.5), at 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57
when incubated for 15 min at pH 7.0
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
IABF_BIFLN
566
0
61548
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
260000
detagged enzyme, gel filtration
64000
4 * 64000, SDS-PAGE
61260
-
mass spectrometry and gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
4 * 64000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Lactococcus lactis by Ni2+-chelate affinity chromatography
Ni2+-charged HiTrap IMAC column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, overexpression of the C-terminally His-tagged enzyme in Lactococcus lactis NZ9000 under control of nisin-inducible nisA promotor
expressed in Escherichia coli BL21(DE3) cells
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
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degradation of lignocellulose, hemicellulose and pectin
food industry
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clarification of fruit juices for wine industry
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Margolles, A.; de los Reyes-Gavillan, C.G.
Purification and functional characterization of a novel alpha-L-arabinofuranosidase from Bifidobacterium longum B667
Appl. Environ. Microbiol.
69
5096-5103
2003
Bifidobacterium longum (Q841V6), Bifidobacterium longum B667 (Q841V6)
Manually annotated by BRENDA team
Numan, M.T.; Bhosle, N.B.
alpha-L-Arabinofuranosidases: the potential applications in biotechnology
J. Ind. Microbiol. Biotechnol.
33
247-260
2006
Aspergillus luchuensis, Aspergillus oryzae, Aspergillus oryzae HL15, Bacillus pumilus, Bacillus pumilus PS213, Bifidobacterium longum, Bifidobacterium longum B667, Brevibacillus brevis, Brevibacillus brevis K-110, Cellvibrio japonicus, Clostridium cellulovorans, Fusarium oxysporum f. sp. dianthi, Penicillium chrysogenum, Rhizomucor pusillus, Rhizomucor pusillus HHT1, Streptomyces chartreusis, Streptomyces chartreusis GS901, Streptomyces thermoviolaceus, Streptomyces thermoviolaceus OP-520, Talaromyces purpureogenus, Thermoanaerobacter ethanolicus, Thermoanaerobacter ethanolicus JW 200, Thermobacillus xylanilyticus, Thermobacillus xylanilyticus D3, Thermobifida fusca, Thermomicrobia bacterium PRI-1686, Thermotoga maritima, Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
Manually annotated by BRENDA team
Gueimonde, M.; Noriega, L.; Margolles, A.; de los Reyes-Gavilan, C.G.
Induction of alpha-L-arabinofuranosidase activity by monomeric carbohydrates in Bifidobacterium longum and ubiquity of encoding genes
Arch. Microbiol.
187
145-153
2007
Bifidobacterium longum, Bifidobacterium longum NIZO B667
Manually annotated by BRENDA team
Lee, J.H.; Hyun, Y.J.; Kim, D.H.
Cloning and characterization of alpha-L-arabinofuranosidase and bifunctional alpha-L-arabinopyranosidase/beta-D-galactopyranosidase from Bifidobacterium longum H-1
J. Appl. Microbiol.
111
1097-1107
2011
Bifidobacterium longum (E7CY70), Bifidobacterium longum H-1 (E7CY70)
Manually annotated by BRENDA team