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Information on EC 3.2.1.4 - cellulase and Organism(s) Bursaphelenchus xylophilus and UniProt Accession Q6BCL7

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EC Tree
     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.4 cellulase
IUBMB Comments
Will also hydrolyse 1,4-linkages in beta-D-glucans also containing 1,3-linkages.
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This record set is specific for:
Bursaphelenchus xylophilus
UNIPROT: Q6BCL7
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Word Map
The taxonomic range for the selected organisms is: Bursaphelenchus xylophilus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
cellulase, cel7a, cellobiohydrolase i, cel5a, cel6a, avicelase, endocellulase, celluclast, cel7b, cbhii, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.5 cellulase
-
-
-
-
Abscission cellulase
-
-
-
-
alkali cellulase
-
-
-
-
Alkaline cellulase
-
-
-
-
avicelase
-
-
-
-
beta-1,4-endoglucan hydrolase
-
-
-
-
beta-1,4-glucanase
-
-
-
-
Carboxymethyl cellulase
-
-
-
-
Carboxymethyl-cellulase
-
-
-
-
carboxymethylcellulase
-
-
-
-
CEL1
-
-
-
-
cellobiohydrolase
-
-
-
-
celluase A
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-
-
-
celludextrinase
-
-
-
-
Cellulase
-
-
-
-
cellulase A 3
-
-
-
-
Cellulase E1
-
-
-
-
Cellulase E2
-
-
-
-
Cellulase E4
-
-
-
-
Cellulase E5
-
-
-
-
Cellulase SS
-
-
-
-
Cellulase V1
-
-
-
-
cellulosin AP
-
-
-
-
CMCase
-
-
-
-
CX-cellulase
-
-
-
-
EG1
-
-
-
-
EG2
-
-
-
-
EG3
-
-
-
-
EGA
-
-
-
-
EGB
-
-
-
-
EGC
-
-
-
-
EGCCA
-
-
-
-
EGCCC
-
-
-
-
EGCCD
-
-
-
-
EGCCF
-
-
-
-
EGCCG
-
-
-
-
EGD
-
-
-
-
EGE
-
-
-
-
EGF
-
-
-
-
EGH
-
-
-
-
EGI
-
-
-
-
EGIV
-
-
-
-
EGM
-
-
-
-
EGSS
-
-
-
-
EGX
-
-
-
-
EGY
-
-
-
-
EGZ
-
-
-
-
endo-1,4-beta-D-glucanase
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-
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endo-1,4-beta-glucanase
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-
-
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endo-1,4-beta-glucanase E1
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-
-
-
endo-1,4-beta-glucanase V1
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-
-
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endocellulase E1
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-
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endoglucanase
endoglucanase D
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-
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FI-CMCASE
-
-
-
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pancellase SS
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-
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Thermoactive cellulase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
4-beta-D-glucan 4-glucanohydrolase
Will also hydrolyse 1,4-linkages in beta-D-glucans also containing 1,3-linkages.
CAS REGISTRY NUMBER
COMMENTARY hide
9012-54-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
amorphous cellulose + H2O
?
show the reaction diagram
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
?
glucomannan + H2O
?
show the reaction diagram
-
-
-
?
amorphous cellulose + H2O
?
show the reaction diagram
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
?
glucomannan + H2O
?
show the reaction diagram
-
-
-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.2 - 8.3
the maximum activity range is broad, with 70% of maximum activity occurring between pH 4.2 and 8.3
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60 - 70
more than 80% of maximal activity is observed for Bx-ENG-2 at 70°C
additional information
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q6BCL7_BURXY
286
0
29496
TrEMBL
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18000
25000
Bx-ENG-1 shows a single band with a molecular size of 25 kDa when 2-mercaptethanol is added to the SDS-PAGE sample
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
Bx-ENG-2 exists in a glycosylated dimeric form
dimer
Bx-ENG-3 exists in a glycosylated dimeric form
monomer
Bx-ENG-1 exists in an unglycosylated monomeric form
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Pichia pastoris
expressed in Pichia pastoris
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Shibuya, H.; Kikuchi, T.
Purification and characterization of recombinant endoglucanases from the pine wood nematode Bursaphelenchus xylophilus
Biosci. Biotechnol. Biochem.
72
1325-1332
2008
Bursaphelenchus xylophilus (Q6BCL3), Bursaphelenchus xylophilus (Q6BCL7), Bursaphelenchus xylophilus (Q6BCM1), Bursaphelenchus xylophilus
Manually annotated by BRENDA team