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EC Tree
IUBMB CommentsWill also hydrolyse 1,4-linkages in beta-D-glucans also containing 1,3-linkages.
The taxonomic range for the selected organisms is: Bursaphelenchus xylophilus
The enzyme appears in selected viruses and cellular organisms
Synonyms
cellulase, cel7a, cellobiohydrolase i, cel5a, cel6a, avicelase, endocellulase, celluclast, cel7b, cbhii,
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Abscission cellulase
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Alkaline cellulase
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beta-1,4-endoglucan hydrolase
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beta-1,4-glucanase
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Carboxymethyl cellulase
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Carboxymethyl-cellulase
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carboxymethylcellulase
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cellobiohydrolase
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endo-1,4-beta-D-glucanase
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endo-1,4-beta-glucanase
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endo-1,4-beta-glucanase E1
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endo-1,4-beta-glucanase V1
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Thermoactive cellulase
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endoglucanase
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hydrolysis of O-glycosyl bond
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4-beta-D-glucan 4-glucanohydrolase
Will also hydrolyse 1,4-linkages in beta-D-glucans also containing 1,3-linkages.
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amorphous cellulose + H2O
?
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?
carboxymethyl cellulose + H2O
?
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?
glucomannan + H2O
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?
amorphous cellulose + H2O
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?
carboxymethyl cellulose + H2O
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?
glucomannan + H2O
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?
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4.2 - 8.3
the maximum activity range is broad, with 70% of maximum activity occurring between pH 4.2 and 8.3
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60 - 70
more than 80% of maximal activity is observed for Bx-ENG-2 at 70°C
additional information
more than 80% of maximal activity is observed for Bx-ENG-3 at 70°C
additional information
more than 80% of maximal activity is observed for Bx-ENG-3 at 70°C
additional information
more than 80% of maximal activity is observed for Bx-ENG-3 at 70°C
additional information
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more than 80% of maximal activity is observed for Bx-ENG-3 at 70°C
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UniProt
brenda
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Q6BCL7_BURXY
286
0
29496
TrEMBL
Secretory Pathway (Reliability: 1)
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25000
Bx-ENG-1 shows a single band with a molecular size of 25 kDa when 2-mercaptethanol is added to the SDS-PAGE sample
18000
gel filtration
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dimer
Bx-ENG-2 exists in a glycosylated dimeric form
dimer
Bx-ENG-3 exists in a glycosylated dimeric form
monomer
Bx-ENG-1 exists in an unglycosylated monomeric form
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expressed in Pichia pastoris
expressed in Pichia pastoris
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Shibuya, H.; Kikuchi, T.
Purification and characterization of recombinant endoglucanases from the pine wood nematode Bursaphelenchus xylophilus
Biosci. Biotechnol. Biochem.
72
1325-1332
2008
Bursaphelenchus xylophilus (Q6BCL3), Bursaphelenchus xylophilus (Q6BCL7), Bursaphelenchus xylophilus (Q6BCM1), Bursaphelenchus xylophilus
brenda
Transporter Classification Database (TCDB):
4.D.3.1.6