endoglucanase E1 produces mainly cellobiose and a trace of glucose, endoglucanase E2 produces mainly cellotriose and higher oligomers with some cellobiose and a trace of glucose
endoglucanase E2 produces glucose, cellobiose, cellotriose and higher oligosaccharides. The major end product produced by endoglucanase E1 is cellobiose and small amounts of glucose
the mechanism for enzymatic hydrolysis of cellulose involves synergistic actions by endoglucanase, EC 3.2.1.4, exoglucanase or cellobiohydrolase, EC 3.2.1.91, and beta-glucosidase, EC 3.2.1.21
model of synergistic cooperation between endocellulase Cel9A and exocellulase Cel48a. Endocellulase Cel9A is most effective on fresh bacterial cellulose with a presumably uniform surface at the molecular level. Its processive activity likely erodes the surface and thus reduces its own activity. Cel48A is able to hydrolyze the Cel9A-modified substrate efficiently and replenish the uniform surface required by Cel9A, creating a feedback mechanism
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapor-diffusion method at room temperature. Structure of the complex of the catalytic domain of endoglucanase Cel6A with a nonhydrolyzable substrate analogue that acts as an inhibitor, methylcellobiosyl-4-thio-beta-cellobioside determined at 1.5 A resolution, structure of mutant enzyme Y73S, structure of mutant enzyme Y73S in complex with methylcellobiosyl-4-thio-beta-cellobioside at 1.04 A resolution, structure of mutant enzyme Y73S in complex with cellotetrose at 1.64 A resolution
activity with carboxymethyl cellulose is 0.2% of wild-type activity, activity with phosphoric acid-swollen cellulose is 1.2% of wild-type activity, activity with bacterial microcrystalline cellulose from Acetobacter xylinum is 1.6% of wild-type activity
activity with carboxymethyl cellulose is less than 0.1% of wild-type activity, activity with phosphoric acid-swollen cellulose is 0.13% of wild-type activity, activity with bacterial microcrystalline cellulose from Acetobacter xylinum is 0.5% of wild-type activity
activity with carboxymethyl cellulose is 0.3% of wild-type activity, activity with phosphoric acid-swollen cellulose is 1.6% of wild-type activity, activity with bacterial microcrystalline cellulose from Acetobacter xylinum is 2.2% of wild-type activity
activity with carboxymethyl cellulose is 0.4% of wild-type activity, activity with phosphoric acid-swollen cellulose is 1.8% of wild-type activity, activity with bacterial microcrystalline cellulose from Acetobacter xylinum is 3.5% of wild-type activity, mutant enzyme loses about 90% of the initial activity after 15 h at 65°C, compared to 10% loss of wild-type activity
activity with carboxymethyl cellulose is 0.45% of wild-type activity, activity with phosphoric acid-swollen cellulose is 2% of wild-type activity, activity with bacterial microcrystalline cellulose from Acetobacter xylinum is 2.7% of wild-type activity, mutant enzyme loses about 20% of the initial activity after 15 h at 65°C, compared to 10% loss of wild-type activity
activity with carboxymethyl cellulose, acid-swollen cellulose or bacterial microcrystalline cellulose from Acetobacter xylinum is nearly identical to wild-type activity
activity with carboxymethyl cellulose is 0.13% of wild-type activity, activity with phosphoric acid-swollen cellulose is 0.2% of wild-type activity, activity with bacterial microcrystalline cellulose from Acetobacter xylinum is 0.43% of wild-type activity, kcat/Km for 2,4-dinitrophenyl beta-D-cellobioside is 8.5fold higher than the wild-type value
activity with carboxymethyl cellulose is 0.3% of wild-type activity, activity with phosphoric acid-swollen cellulose is 1.1% of wild-type activity, activity with bacterial microcrystalline cellulose from Acetobacter xylinum is 2.5% of wild-type activity, kcat/Km for 2,4-dinitrophenyl beta-D-cellobioside is 123.8fold higher than the wild-type value
activity with carboxymethyl cellulose is less than 0.1% of wild-type activity, activity with phosphoric acid-swollen cellulose is 0.15% of wild-type activity, activity with bacterial microcrystalline cellulose from Acetobacter xylinum is 1.1% of wild-type activity
activity with carboxymethyl cellulose is 7% of wild-type activity, activity with phosphoric acid-swollen cellulose is 4.1% of wild-type activity, activity with bacterial microcrystalline cellulose from Acetobacter xylinum is 8.3% of wild-type activity, mutant enzyme loses about 20% of the initial activity after 15 h at 65°C, compared to 10% loss of wild-type activity
activity with carboxymethyl cellulose is 0.5% of wild-type activity, activity with phosphoric acid-swollen cellulose is 1.3% of wild-type activity, activity with bacterial microcrystalline cellulose from Acetobacter xylinum is 1.8% of wild-type activity
activity with carboxymethyl cellulose is 5fold higher than wild-type activity, activity with phosphoric acid-swollen cellulose is 28% of wild-type activity, activity with bacterial microcrystalline cellulose from Acetobacter xylinum is 14.2% of wild-type activity, mutant enzyme loses about 30% of the initial activity after 15 h at 65°C, compared to 10% loss of wild-type activity
activity with carboxymethyl cellulose is 6.7fold higher than wild-type activity, activity with phosphoric acid-swollen cellulose is 75% of wild-type activity, activity with bacterial microcrystalline cellulose from Acetobacter xylinum is 16.6% of wild-type activity
Cel9A mutant, causes weaker binding to alpha-chitin than wild-type, mutation of residue near the catalytic center. Mutant has weak chitinase activity, but no soluble products are detected
activity with phosphoric acid-swollen cellulose is less than 1.3% of the wild-type activity, activity with carboxymethylcellulose is less than 0.9% of the wild-type activity, activity with bacterial microcrystalline cellulose is less than 18.7% of the wild-type activity
activity with phosphoric acid-swollen cellulose is 54% of the wild-type activity, activity with carboxymethylcellulose is 15% of the wild-type activity, activity with bacterial microcrystalline cellulose is 52% of the activity with wild-type enzyme
15 h, wild-type enzyme is stable, mutant enzyme D58A loses about 20% of initial activity, mutant enzymes Y206F, D58N, D58N and Y318A lose about 10% of the inital activity
15 h, wild-type enzyme loses about 10% of initial activity, mutant enzyme D58N and Y206F loses about 20% of maximal activity, mutant enzyme D58A loses about 90% of initial activity, mutant enzyme Y318A loses about 30% of initial activity
Cel6A production in Escherichia coli and the DB-Cel6A fusions are inserted by particle bombardment method into the tobacco (Nicotiana tabacum cv. Samsun) chloroplast genome for protein expression
Crystal structure of Thermobifida fusca endoglucanase Cel6A in complex with substrate and inhibitor: the role of tyrosine Y73 in substrate ring distortion
Batista, P.R.; Costa, M.G.; Pascutti, P.G.; Bisch, P.M.; de Souza, W.
High temperatures enhance cooperative motions between CBM and catalytic domains of a thermostable cellulase: mechanism insights from essential dynamics