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Information on EC 3.2.1.36 - hyaluronoglucuronidase and Organism(s) Streptococcus pyogenes phage H10403 and UniProt Accession Q54699

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Streptococcus pyogenes phage H10403
UNIPROT: Q54699 not found.
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The taxonomic range for the selected organisms is: Streptococcus pyogenes phage H10403
The enzyme appears in selected viruses and cellular organisms
Synonyms
hyaluronidase, haase, hyase, haase-b, vest2a, vest2b, lhase, hyaluronate lyase hylp2, hyaluronoglucuronidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hyaluronate lyase HylP2
phage-encoded
glucuronoglucosaminoglucan hyaluronate lyase
-
-
-
-
hyaluronidase
-
-
-
-
orgelase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
hyaluronate 3-glycanohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
37288-34-9
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
ascorbic acid
Streptococcus pyogenes phage H10403
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HYLP2_BPH45
337
0
36453
Swiss-Prot
-
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotrimer
gel filtration, cross-linking experiments, 110 kDa under physiological conditions
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop vapour diffusion method
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Y264F
involved in catalytic activity, mutant protein without activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni2+ chelate chromatography, gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
His-tagged version expressed in Escherichia coli BL21
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mishra, P.; Prem Kumar, R.; Ethayathulla, A.S.; Singh, N.; Sharma, S.; Perbandt, M.; Betzel, C.; Kaur, P.; Srinivasan, A.; Bhakuni, V.; Singh, T.P.
Polysaccharide binding sites in hyaluronate lyase--crystal structures of native phage-encoded hyaluronate lyase and its complexes with ascorbic acid and lactose
FEBS J.
276
3392-3402
2009
Streptococcus pyogenes phage H10403 (Q54699)
Manually annotated by BRENDA team