Any feedback?
Information on EC 3.2.1.35 - hyaluronoglucosaminidase and Organism(s) Mus musculus and UniProt Accession Q8VEI3
for references in articles please use BRENDA:EC3.2.1.35Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
3.2.1.35 hyaluronoglucosaminidaseIUBMB Comments
Also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates, and dermatan.
Specify your search results
Word Map
- 3.2.1.35
- glycosaminoglycans
- proteoglycans
- hyaluronan
- cartilage
- collagen
- heparan
- hyaluronidases
- disaccharide
- oligosaccharide
- dermatan
- fibronectin
- chondrocytes
- heparitinase
-
articular
-
hyals
-
alcian
-
cspgs
-
acrosomal
- chabc
- aggrecan
- 4-sulfate
- tetrasaccharide
-
35ssulfate
-
cumulus
-
mucopolysaccharides
- haase
-
pericellular
- keratanase
- pellucida
-
6-sulphate
- hexasaccharide
-
transglycosylation
- diagnostics
- chlorate
- chondroitin-6-sulfate
-
rhamm
-
ha-binding
-
monosulfated
-
hexuronic
-
cetylpyridinium
-
d-glucuronic
- molecular biology
-
acrosome-reacted
-
iduronic
- biotechnology
-
glca
-
3hglucosamine
- pharmacology
-
perineuronal
- analysis
- neurocan
- disulfated
- octasaccharide
-
tgfbr3
- heparinum
- medicine
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
chondroitinase, testicular hyaluronidase, hyal1, hyal2, ph-20, hyal-1, rhuph20, spreading factor, hyal-2, mgea5, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
chondritinase I
-
-
-
-
chondroitinase
-
-
-
-
hyaluronidase
hyaluronoglucosidase
-
-
-
-
Mu toxin
-
-
-
-
Sperm surface protein PH-20
-
-
-
-
hyaluronidase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate
Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate
also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates, and dermatan
-
Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate
reaction mechanism and three-dimensional structure
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
hyaluronate 4-glycanohydrolase
Also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates, and dermatan.
CAS REGISTRY NUMBER
COMMENTARY
37326-33-3
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
hyaluronan + 2 H2O
N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
-
-
?
hyaluronan + H2O
N-acetylglucosamine + D-glucuronate + ?
-
complete degradation, absolutely specific for
-
?
hyaluronic acid + H2O
?
-
two isoforms necessary for penetration of extracellular vestments surrounding eggs prior fertilization
-
-
?
hyaluronan + 2 H2O
N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
-
-
?
additional information
?
-
-
CD44-mediated specific role of certain populations of testicular enzyme in the induction of angiogenesis
-
?
additional information
?
-
-
enzyme activity in serum from tumour-bearing mice, e.g. H460M or SA87 cell grafted mice, is increased, the level is corrsponding to the tumour mass, not in serum from CB193 cell grafted mice
-
?
additional information
?
-
-
the enzyme depolymerizes various chondroitin sulfate isoforms to a similar extent, suggesting broad substrate specificity
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
hyaluronic acid + H2O
?
-
two isoforms necessary for penetration of extracellular vestments surrounding eggs prior fertilization
-
-
?
hyaluronan + 2 H2O
N-acetyl-beta-D-glucosamine + D-glucuronate + ?
-
-
-
?
additional information
?
-
-
CD44-mediated specific role of certain populations of testicular enzyme in the induction of angiogenesis
-
?
additional information
?
-
-
enzyme activity in serum from tumour-bearing mice, e.g. H460M or SA87 cell grafted mice, is increased, the level is corrsponding to the tumour mass, not in serum from CB193 cell grafted mice
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
blocking of CD44-receptors inhibits the enzyme-induced tubular structure formation of endothelial cells
-
additional information
-
the ethanol extract of of the stem bark of Butea monosperma dose-dependently inhibits enzyme with IC50 of 0.0494 mg/ml
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
cells form tubular structures after stimulation by angiogenic factors, e.g. the enzyme and the basic fibroblast growth factor bFGF, treatment leads to cell survival and prevents from apoptosis, respectively
-
Hyal3 is expressed in epididymal tissue/fluid, from where it is acquired by caudal mouse sperm in vitro
-
from mice grafted with human-tumour-derived, enzyme secreting H460M or SA87 cells, and mice grafted with enzyme non-secreting CB 193 cells
-
Hyal3 is expressed in epididymal tissue/fluid, from where it is acquired by caudal mouse sperm in vitro
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
mouse isoforms are differentially distributed in the soluble, membrane, and acrosome-related fraction, where they are most abundant
-
-
mouse isoforms are differentially distributed in the soluble, membrane, and acrosome-related fraction, where they are most abundant
-
-
mouse isoforms are differentially distributed in the soluble, membrane, and acrosome-related fraction, where they are most abundant. Protein resides in the membrane over the sperm head and midpiece
-
protein resides in the membrane over the sperm head and midpiece
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
HYAL5-deficient epididymal sperm is functionally normal, the loss of SPAM1 results in a remarkably increased accumulation of sperm on the surface or outer edge of the cumulus
physiological function
physiological function
-
SPAM1 is involved in sperm penetration through the cumulus matrix
physiological function
-
although fully fertile, Hyal3 null sperm shows delayed cumulus penetration and reduced acrosomal exocytosis. Spam1 null mutants lacking sperm adhesion molecule 1 have significantly diminished Hyal3 activity
physiological function
-
the enzyme play a special role in the testes of adult mice being involved in the maturation of sperm or during fertilization
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). HYAL3_MOUSE
412
0
46132
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
47000
55000
additional information
additional information
presence of multiple forms of Hyal3 ranging from about 45000 to 56000 Da in expression lysates
additional information
-
presence of multiple forms of Hyal3 ranging from about 45000 to 56000 Da in expression lysates
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
-
cancer cell lines often secrete hyaluronidase, the enzyme might therefore be a marker for growing tumours
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Thaler, C.D.; Cardullo, R.A.
Biochemical characterization of a glycosylphosphatidyl-inositol-linked hyaluronidase on mouse sperm
Biochemistry
34
7788-7795
1995
Mus musculus
Maingonnat, C.; Courel, M.N.; Bertrand, P.; Vincent, J.C.; Sesboue, R.; Delpech, B.
Hyaluronidase in sera of tumour-bearing nude mice
Biomarkers
8
333-338
2003
Mus musculus
Rahmanian, M.; Heldin, P.
Testicular hyaluronidase induces tubular structures of endothelial cells grown in three-dimensional collagen gel through a CD44-mediated mechanism
Int. J. Cancer
97
601-607
2002
Mus musculus
Stern, R.; Jedrzejas, M.J.
Hyaluronidases: their genomics, structures, and mechanisms of action
Chem. Rev.
106
818-839
2006
Bos taurus, Homo sapiens, Mus musculus, Apis mellifera (Q08169)
Hemming, R.; Martin, D.C.; Slominski, E.; Nagy, J.I.; Halayko, A.J.; Pind, S.; Triggs-Raine, B.
Mouse Hyal3 encodes a 45-56 kDa glycoprotein whose overexpression increases hyaluronidase 1 activity in cultured cells
Glycobiology
18
280-289
2008
Mus musculus (Q8VEI3), Mus musculus
de la Motte, C.; Nigro, J.; Vasanji, A.; Rho, H.; Kessler, S.; Bandyopadhyay, S.; Danese, S.; Fiocchi, C.; Stern, R.
Platelet-derived hyaluronidase 2 cleaves hyaluronan into fragments that trigger monocyte-mediated production of proinflammatory cytokines
Am. J. Pathol.
174
2254-2264
2009
Mus musculus (O35632), Homo sapiens (Q12891)
Kimura, M.; Kim, E.; Kang, W.; Yamashita, M.; Saigo, M.; Yamazaki, T.; Nakanishi, T.; Kashiwabara, S.; Baba, T.
Functional roles of mouse sperm hyaluronidases, HYAL5 and SPAM1, in fertilization
Biol. Reprod.
81
939-947
2009
Mus musculus
Reese, K.L.; Aravindan, R.G.; Griffiths, G.S.; Shao, M.; Wang, Y.; Galileo, D.S.; Atmuri, V.; Triggs-Raine, B.L.; Martin-Deleon, P.A.
Acidic hyaluronidase activity is present in mouse sperm and is reduced in the absence of SPAM1: evidence for a role for hyaluronidase 3 in mouse and human sperm
Mol. Reprod. Dev.
77
759-772
2010
Mus musculus
Kaneiwa, T.; Miyazaki, A.; Kogawa, R.; Mizumoto, S.; Sugahara, K.; Yamada, S.
Identification of amino acid residues required for the substrate specificity of human and mouse chondroitin sulfate hydrolase (conventional hyaluronidase-4)
J. Biol. Chem.
287
42119-42128
2012
Homo sapiens, Mus musculus
EXTERNAL LINKS (specific for EC-Number 3.2.1.35)
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
