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Information on EC 3.2.1.35 - hyaluronoglucosaminidase and Organism(s) Apis mellifera and UniProt Accession Q08169

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IUBMB Comments
Also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates, and dermatan.
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This record set is specific for:
Apis mellifera
UNIPROT: Q08169
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Word Map
The taxonomic range for the selected organisms is: Apis mellifera
The enzyme appears in selected viruses and cellular organisms
Synonyms
chondroitinase, testicular hyaluronidase, hyal1, hyal2, ph-20, hyal-1, rhuph20, spreading factor, hyal-2, mgea5, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
chondritinase I
-
-
-
-
chondroitinase
-
-
-
-
hyaluronidase
hyaluronoglucosidase
-
-
-
-
Mu toxin
-
-
-
-
Sperm surface protein PH-20
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
hyaluronate 4-glycanohydrolase
Also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates, and dermatan.
CAS REGISTRY NUMBER
COMMENTARY hide
37326-33-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-S-chondroitin sulfate + H2O
?
show the reaction diagram
-
-
-
?
6-S-chondroitin sulfate + H2O
?
show the reaction diagram
the enzyme is capable of degrading chondroitin sulfate C at a rate of around 40% compared to its hyaluronidase activity
-
-
?
chondroitin B sulfate + H2O
?
show the reaction diagram
very little hydrolase activity towards chondroitin sulfate B (dermatan sulfate)
-
-
?
chondroitin sulfate A + H2O
?
show the reaction diagram
-
-
-
?
chondroitin sulfate B + H2O
?
show the reaction diagram
very little hydrolase activity
-
-
?
chondroitin sulfate C + H2O
?
show the reaction diagram
-
-
-
?
hyaluronan + 2 H2O
N-acetyl-beta-D-glucosamine + D-glucuronate + ?
show the reaction diagram
-
-
-
?
hyaluronan + H2O
hyaluronan oligomers
show the reaction diagram
-
-
-
?
hyaluronate + H2O
N-acetyl-beta-D-glucosamine + D-glucuronate + ?
show the reaction diagram
-
-
?
hyaluronic acid + H2O
?
show the reaction diagram
-
-
-
?
hyaluronic acid + H2O
?
show the reaction diagram
additional information
?
-
the enzyme is a major allergen of bee venom which can induce serious, occasionally fatal, systemic IgE-mediated anaphylactic reactions in humans
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
hyaluronan + H2O
hyaluronan oligomers
show the reaction diagram
-
-
-
?
hyaluronate + H2O
N-acetyl-beta-D-glucosamine + D-glucuronate + ?
show the reaction diagram
-
-
?
hyaluronic acid + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
the enzyme is a major allergen of bee venom which can induce serious, occasionally fatal, systemic IgE-mediated anaphylactic reactions in humans
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glutathione
non-competitive
N-acetyl-L-cysteine
non-competitive
beta1,4-galacto-oligosaccharides
-
partially sulfated and non-sulfated forms, especially tri-, tetra-, and pentasaccharides
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partially sulfated neomycin
-
the non-sulfated neomycin is not inhibitory
-
partially sulfated planteose
-
the non-sulfated planteose is not inhibitory, IC50 is 0.008 mM
partially sulfated verbascose
-
2 forms, the non-sulfated verbascose is not inhibitory, IC50 are 0.040 mM and 0.003 mM
-
sulfated hydroquinone galactoside
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IC50 is 0.006 mM
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.008
partially sulfated planteose
Apis mellifera
-
the non-sulfated planteose is not inhibitory, IC50 is 0.008 mM
0.04
partially sulfated verbascose
Apis mellifera
-
2 forms, the non-sulfated verbascose is not inhibitory, IC50 are 0.040 mM and 0.003 mM
-
0.006
sulfated hydroquinone galactoside
Apis mellifera
-
IC50 is 0.006 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000245
recombinant enzyme from crude extract, in 100 mM sodium phosphate, pH 5.0, at 37°C
0.000239
recombiannt enzyme after 9.6fold purification, in 100 mM sodium phosphate, pH 5.0, at 37°C
0.49
culture supernatant
4.77
ion exchange
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 90
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HUGA_APIME
382
1
44260
Swiss-Prot
Secretory Pathway (Reliability: 1)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
three-dimensional structure analysis
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
8-10 mg/ml purified recombinant enzyme, with or wihtout hexameric hyaluronic acid, in 5 mM sodium acetate, pH 5.4, hanging drop vapour diffusion method, 20°C, for triclinic crystals, equal volume of protein solution with reservoir solution containing 30% PEG 8000, 0.1 M sodium cacodylate, pH 6.5, 0.2 M ammonium sulfate, equilibration against the reservoir solution, 6-8 months, for monoclinic crystal growth within several days, 10% PEG 6000, 1.0 M NaCl, 20 mM acetic acid, pH 4.2 is used for mixing and equilibration, X-ray diffraction structure determination and analysis at 1.6-2.7 A resolution
three-dimensional structure analysis
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 8
native and recombinant enzyme exhibit activity
682687
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 90
34% activity at temperatures as low as 4°C still exhibits hydrolase activity at 90°C to an extent of 19% in comparison to the optimum
90
19% activity at 90°C
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by ion exchange chromatography at pH 5.0
HiTrap HP SP column chromatography
recombinant His-tagged enzyme
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence analysis, expression as active C-terminally His-tagged enzyme in insect cells via baculovirus infection
expressed in Pichia pastoris strain GS115
expression in Pichia pastoris GS115, expression vector pPIC9, together with human serum albumin, expression in Escherichia coli BL21 DE3, vector pMW172, fusion protein
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Markovic-Housley, Z.; Miglierini, G.; Soldatova, L.; Rizkallah, P.J.; Muller, U.; Schirmer, T.
Crystal structure of hyaluronidase, a major allergen of bee venom
Structure
8
1025-1035
2000
Apis mellifera (Q08169)
Manually annotated by BRENDA team
Stern, R.; Jedrzejas, M.J.
Hyaluronidases: their genomics, structures, and mechanisms of action
Chem. Rev.
106
818-839
2006
Bos taurus, Homo sapiens, Mus musculus, Apis mellifera (Q08169)
Manually annotated by BRENDA team
Salmen, S.; Hoechstetter, J.; Kaesbauer, C.; Paper, D.H.; Bernhardt, G.; Buschauer, A.
Sulphated oligosaccharides as inhibitors of hyaluronidases from bovine testis, bee venom and Streptococcus agalactiae
Planta Med.
71
727-732
2005
Apis mellifera, Bos taurus
Manually annotated by BRENDA team
Reitinger, S.; Boroviak, T.; Laschober, G.T.; Fehrer, C.; Muellegger, J.; Lindner, H.; Lepperdinger, G.
High-yield recombinant expression of the extremophile enzyme, bee hyaluronidase in Pichia pastoris
Protein Expr. Purif.
57
226-233
2008
synthetic construct (EU152302), Apis mellifera (Q08169)
Manually annotated by BRENDA team
Sunitha, K.; Suresh, P.; Santhosh, M.S.; Hemshekhar, M.; Thushara, R.M.; Marathe, G.K.; Thirunavukkarasu, C.; Kemparaju, K.; Kumar, M.S.; Girish, K.S.
Inhibition of hyaluronidase by N-acetyl cysteine and glutathione: role of thiol group in hyaluronan protection
Int. J. Biol. Macromol.
55
39-46
2013
Bos taurus, Echis carinatus, Homo sapiens, Apis mellifera (Q08169)
Manually annotated by BRENDA team