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Information on EC 3.2.1.33 - amylo-alpha-1,6-glucosidase and Organism(s) Escherichia coli and UniProt Accession P15067

for references in articles please use BRENDA:EC3.2.1.33
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EC Tree
IUBMB Comments
This enzyme hydrolyses an unsubstituted glucose unit linked by an alpha(1->6) bond to an alpha(1->4) glucose chain. The enzyme activity found in mammals and yeast is in a polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25 (4-alpha-glucanotransferase), which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can then hydrolyse. Together, these two activities constitute the glycogen debranching system.
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This record set is specific for:
Escherichia coli
UNIPROT: P15067
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Word Map
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
amylo-1,6-glucosidase, debrancher enzyme, amylo-alpha-1,6-glucosidase, debrancher protein, amylo-alpha-1,6-glucosidase activity, amylo-1,6-glucosidase/4-alpha-glucanotransferase, dextrin 6-glucohydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1,4-glucan-4-glucosyltransferase/amylo-1,6-glucosidase
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4-alpha-glucano-transferase amylo-1,6-glucosidase
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alpha-(1,6)-glucosidase
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amylo-1,6-glucosidase
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amylo-1,6-glucosidase-oligo-1,4-1,4-transferase
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amylo-1,6-glucosidase/1,4-alpha-glucan 4-alpha-glucan 4-alpha-glycosyltransferase
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amylo-1,6-glucosidase/1,4-alpha-glucan:1,4-alpha-glucan 4-alpha-glycosyltransferase
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amylo-1,6-glucosidase/4-alpha-glucanotransferase
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amylo-1,6-glucosidase/oligo-1,4-1,4-glucantransferase
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amylopectin 1,6-glucosidase
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debrancher enzyme
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debrancher protein
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dextrin 6-alpha-D-glucosidase
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dextrin 6-alpha-glucosidase
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dextrin 6-glucanohydrolase
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dextrin 6-glucohydrolase
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dextrin-1,6-glucosidase
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dextrin:6-glucohydrolase
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glucosidase, amylo-1,6-
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glucosidase/transferase
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glycogen debranching enzyme
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glycogen phosphorylase limit dextrin debranching system
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indirect debranching enzyme
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oligo-alpha-1,4-glucan:alpha-1,4-glucan-4-glycosyltransferase-amylo-1,6-glucosidase
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transferase-glucosidase
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
glycogen phosphorylase-limit dextrin 6-alpha-glucohydrolase
This enzyme hydrolyses an unsubstituted glucose unit linked by an alpha(1->6) bond to an alpha(1->4) glucose chain. The enzyme activity found in mammals and yeast is in a polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25 (4-alpha-glucanotransferase), which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can then hydrolyse. Together, these two activities constitute the glycogen debranching system.
CAS REGISTRY NUMBER
COMMENTARY hide
9012-47-9
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
300
beta-cyclodextrin-alpha-1,6-linked maltotetraose
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pH 7, 37°C
26
beta-cyclodextrin-alpha-1,6-linked maltotriose
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pH 7, 37°C
26
G3-beta-cyclodextrin
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in 50 mM sodium phosphate buffer (pH7.0) at 37°C
300
G4-beta-cyclodextrin
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in 50 mM sodium phosphate buffer (pH7.0) at 37°C