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Information on EC 3.2.1.31 - beta-glucuronidase and Organism(s) Saccharolobus solfataricus and UniProt Accession Q97UI1

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Saccharolobus solfataricus
UNIPROT: Q97UI1 not found.
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Word Map
The taxonomic range for the selected organisms is: Saccharolobus solfataricus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
beta-glucuronidase, beta-d-glucuronidase, betag, beta-gluc, beta-glucuronidase a, hgusb, exo-beta-glucuronidase, tmgusi, exo-beta-d-glucuronidase, ketodase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Beta-D-glucuronoside glucuronosohydrolase
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Beta-G1
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exo-beta-D-glucuronidase
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glucuronidase, beta-glucuronide glucuronohydrolase
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GUS
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ketodase
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SYSTEMATIC NAME
IUBMB Comments
beta-D-glucuronoside glucuronosohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
9001-45-0
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
show the reaction diagram
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-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.54 - 0.58
4-methylumbelliferyl beta-D-glucuronide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 6.5
highly active in the pH-range 4.5-6.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37 - 100
37°C: about 40% of maximal activity, 100°C: about 50% of maximal activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65
4.5 d, 1.1% loss of activity
70
10 h, enzyme retains full activity
80
10 h, enzyme retains full activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli. Although Escherichia coli possesses an endogenous beta-glucuronidase activity, a short treatment at 80°C of the crude extract causes the precipitation of most Escherichia coli protein, resulting in an enrichment of the Sulfolobus beta-glucuronidase
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
Sulfolobus gene can be used as reporter in any thermophilic microorganism lacking an endogenous beta-glucuronidase activity
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Honarbakhsh, M.; Villafane, A.A.; Ruhl, I.; Sannino, D.; Bini, E.
Development of a thermostable beta-glucuronidase-based reporter system for monitoring gene expression in hyperthermophiles
Biotechnol. Bioeng.
109
1881-1886
2012
Saccharolobus solfataricus (Q97UI1), Saccharolobus solfataricus P2 (Q97UI1)
Manually annotated by BRENDA team