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Information on EC 3.2.1.31 - beta-glucuronidase and Organism(s) Neurospora crassa and UniProt Accession Q7SFB0

for references in articles please use BRENDA:EC3.2.1.31
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This record set is specific for:
Neurospora crassa
UNIPROT: Q7SFB0 not found.
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Word Map
The taxonomic range for the selected organisms is: Neurospora crassa
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
beta-glucuronidase, beta-d-glucuronidase, betag, beta-gluc, beta-glucuronidase a, hgusb, exo-beta-glucuronidase, tmgusi, exo-beta-d-glucuronidase, ketodase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Beta-D-glucuronoside glucuronosohydrolase
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-
-
-
Beta-G1
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-
-
-
exo-beta-D-glucuronidase
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-
-
-
glucuronidase, beta-glucuronide glucuronohydrolase
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-
-
-
GUS
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-
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ketodase
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
beta-D-glucuronoside glucuronosohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
9001-45-0
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methyl-beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O
4-methyl-D-glucuronate + beta-Gal(1-6)Gal
show the reaction diagram
-
4.8% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-glucuronic acid + H2O
4-nitrophenol + D-glucuronic acid
show the reaction diagram
-
-
-
?
beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O
D-glucuronate + beta-Gal(1-6)Gal
show the reaction diagram
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67.4% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
?
GlcA-beta(1-6)Gal + H2O
D-glucuronate + D-galactopyranose
show the reaction diagram
49.9% of the activity with 4-nitrophenyl beta-D-glucuronic acid
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-
?
additional information
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0383
4-nitrophenyl beta-D-glucoside
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0.378
GlcAbeta(1-6)Gal
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29.3
pH 6.0, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
activity gradually decreases below
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58123
x * 58123, calculated, mature protein, x * 90100, SDS-PAGE
90100
x * 58123, calculated, mature protein, x * 90100, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 58123, calculated, mature protein, x * 90100, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
sequence contains 12 putative glycosylation sites
proteolytic modification
sequence contains a putative signal sequence, calculated molecular mass of immature protein is 60235
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
stable
697118
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
30 min, 50% loss of activity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Pichia pastoris
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Konishi, T.; Kotake, T.; Soraya, D.; Matsuoka, K.; Koyama, T.; Kaneko, S.; Igarashi, K.; Samejima, M.; Tsumuraya, Y.
Properties of family 79 beta-glucuronidases that hydrolyze beta-glucuronosyl and 4-O-methyl-beta-glucuronosyl residues of arabinogalactan-protein
Carbohydr. Res.
343
1191-1201
2008
Aspergillus niger, Neurospora crassa (Q7SFB0), Neurospora crassa
Manually annotated by BRENDA team