Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.2.1.31 - beta-glucuronidase and Organism(s) Mus musculus and UniProt Accession P12265

for references in articles please use BRENDA:EC3.2.1.31
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Mus musculus
UNIPROT: P12265 not found.
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
beta-glucuronidase, beta-d-glucuronidase, betag, beta-gluc, beta-glucuronidase a, hgusb, exo-beta-glucuronidase, tmgusi, exo-beta-d-glucuronidase, ketodase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Beta-D-glucuronoside glucuronosohydrolase
-
-
-
-
Beta-G1
-
-
-
-
exo-beta-D-glucuronidase
-
-
-
-
glucuronidase, beta-glucuronide glucuronohydrolase
-
-
-
-
ketodase
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
beta-D-glucuronoside glucuronosohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9001-45-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl beta-D-glucuronide + H2O
4-methylumbelliferone + beta-D-glucuronic acid
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronic acid
show the reaction diagram
-
-
-
-
?
curcumin beta-D-glucuronide + H2O
curcumin + beta-D-glucuronic acid
show the reaction diagram
-
-
-
?
curcumin-beta-D-glucuronide + H2O
curcumin + beta-D-glucuronic acid
show the reaction diagram
curcumin, despite low systemic bioavailability, may be enzymatically activated (deconjugated) within GUSB-enriched bone to exert protective effects, a metabolic process that can also contribute to bone-protective effects of other highly glucuronidated dietary polyphenols
-
-
?
phenolphthalein beta-D-glucuronide + H2O
phenolphthalein + beta-D-glucuronate
show the reaction diagram
-
determination of specific activity in betaG-expressing tumour CT26 cells using radio-labeled substrate
-
-
?
phenolphthalein beta-D-glucuronide + H2O
phenolphthalein + D-glucuronic acid
show the reaction diagram
-
-
-
-
?
phenolphthalein-beta-D-glucuronide + H2O
phenolphthalein + D-glucuronic acid
show the reaction diagram
-
-
-
-
?
retinyl-beta-glucuronide + H2O
retinol + D-glucuronide
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the beta-glucuronidase forms a complex with esterase 22 in the liver. Gus colocalizes with Es22 at the endoplasmic reticulum but does not affect its retinoyl ester hydrolase activity
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
curcumin-beta-D-glucuronide + H2O
curcumin + beta-D-glucuronic acid
show the reaction diagram
curcumin, despite low systemic bioavailability, may be enzymatically activated (deconjugated) within GUSB-enriched bone to exert protective effects, a metabolic process that can also contribute to bone-protective effects of other highly glucuronidated dietary polyphenols
-
-
?
retinyl-beta-glucuronide + H2O
retinol + D-glucuronide
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the beta-glucuronidase forms a complex with esterase 22 in the liver. Gus colocalizes with Es22 at the endoplasmic reticulum but does not affect its retinoyl ester hydrolase activity
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-saccharic acid 1,4-lactone monohydrate
-
a betaG-specific inhibitor
saccharolactone
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.018 - 3.08
phenolphthalein beta-D-glucuronide
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
200
4-nitrophenyl beta-D-glucuronide
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 4.5
-
-
8
-
assay at, recombinant RFP-tagged enzyme
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
expresses only the lysosomal form of the enzyme
Manually annotated by BRENDA team
-
activity in round spermatids is detected in multivesicular bodies and acrosomal vesicles, activity in elongated spermatids is detected in sickle-shaped acrosome and in the residual body
Manually annotated by BRENDA team
-
activity in lysosome-like bodies
Manually annotated by BRENDA team
-
activity in acrosome and in cytoplasmic droplets
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
additional information
-
CT26/betaG cell, colon carcinoma CT26 cells expressing beta-G
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
activity in acrosome and in cytoplasmic droplets. Activity in round spermatids is detected in acrosomal vesicles, activity in elongated spermatids is detected in sickle-shaped acrosome
-
Manually annotated by BRENDA team
-
activity in acrosome and in cytoplasmic droplets
Manually annotated by BRENDA team
-
the beta-glucuronidase forms a complex with esterase 22 in the liver. Gus colocalizes with Es22 at the endoplasmic reticulum but does not affect its RE hydrolase activity
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
beta-glucuronidase forms a complex with esterase 22 in the liver, both esterase Es22 and Gus play a role in liver retinoid metabolism
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
BGLR_MOUSE
648
1
74195
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
250000 - 310000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
mouse, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 11
-
-
26867
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
-
10 min, inactivation at pH 5.0, not at pH 7.0
71
-
30 min, pH 5.0, 80% activity, 30 min, pH 8.0, 20% activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing/thawing, no inactivation
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C or 4°C, 0.02 M sodium acetate buffer, pH 5.2, 0.15 M NaCl, 1 mg/ml protein, several months, sucrose prevents aggregation
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in 3T3 fibroblasts and EJ carcinoma cells
-
expression of red fluorescent protein, RFP, fusion Gus in COS-7 cells, coexpression with GFP-tagged esterase 22
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mills, N.C.; Gupta, C.; Bardin, C.W.
Purification of beta-glucuronidase from urine of androgen-stimulated female mice
Arch. Biochem. Biophys.
185
100-107
1978
Mus musculus
Manually annotated by BRENDA team
Tomino, S.; Paigen, K.
Purification and chemical properities of mouse liver lysosomal (L form) beta-glucuronidase
J. Biol. Chem.
250
8503-8509
1975
Mus musculus
Manually annotated by BRENDA team
Lin, C.W.; Orcutt, M.L.; Fishman, W.H.
Purification and characterization of mouse kidney beta-glucuronidase
J. Biol. Chem.
250
4737-4743
1975
Mus musculus
Manually annotated by BRENDA team
Abou-Haila, A.; Fouquet, J.P.; Tulsiani, D.R.P.
Characterization and immunolocalization of beta-D-glucuronidase in mouse testicular germ cells and spermatozoa
Exp. Cell Res.
247
48-60
1999
Mus musculus
Manually annotated by BRENDA team
Chen, K.C.; Cheng, T.L.; Leu, Y.L.; Prijovich, Z.M.; Chuang, C.H.; Chen, B.M.; Roffler, S.R.
Membrane-localized activation of glucuronide prodrugs by beta-glucuronidase enzymes
Cancer Gene Ther.
14
187-200
2007
Escherichia coli, Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Schreiber, R.; Taschler, U.; Wolinski, H.; Seper, A.; Tamegger, S.N.; Graf, M.; Kohlwein, S.D.; Haemmerle, G.; Zimmermann, R.; Zechner, R.; Lass, A.
Esterase 22 and beta-glucuronidase hydrolyze retinoids in mouse liver
J. Lipid Res.
50
2514-2523
2009
Mus musculus
Manually annotated by BRENDA team
Tzou, S.C.; Roffler, S.; Chuang, K.H.; Yeh, H.P.; Kao, C.H.; Su, Y.C.; Cheng, C.M.; Tseng, W.L.; Shiea, J.; Harm, I.H.; Cheng, K.W.; Chen, B.M.; Hwang, J.J.; Cheng, T.L.; Wang, H.E.
Micro-PET imaging of beta-glucuronidase activity by the hydrophobic conversion of a glucuronide probe
Radiology
252
754-762
2009
Mus musculus
Manually annotated by BRENDA team
Kunihiro, A.G.; Luis, P.B.; Brickey, J.A.; Frye, J.B.; Chow, H.S.; Schneider, C.; Funk, J.L.
beta-Glucuronidase catalyzes deconjugation and activation of curcumin-glucuronide in bone
J. Nat. Prod.
82
500-509
2019
Mus musculus (P12265)
Manually annotated by BRENDA team