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Information on EC 3.2.1.24 - alpha-mannosidase and Organism(s) Picrophilus torridus and UniProt Accession Q6L2X5

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IUBMB Comments
Also hydrolyses alpha-D-lyxosides and heptopyranosides with the same configuration at C-2, C-3 and C-4 as mannose.
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This record set is specific for:
Picrophilus torridus
UNIPROT: Q6L2X5
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Word Map
The taxonomic range for the selected organisms is: Picrophilus torridus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
alpha-mannosidase, mannanase, alpha-d-mannosidase, lysosomal alpha-mannosidase, alpha-mannosidase ii, alpha1,2-mannosidase, golgi alpha-mannosidase ii, alpha-1,2-mannosidase, alpha-man, man2c1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha-mannosidase
-
1,2-alpha-D-mannosidase
-
-
-
-
1,2-alpha-mannosidase
-
-
-
-
Alpha mannosidase 6A8B
-
-
-
-
alpha-D-mannopyranosidase
-
-
-
-
alpha-D-mannosidase
-
-
-
-
Alpha-D-mannoside mannohydrolase
-
-
-
-
AMAN
-
-
-
-
exo-alpha-mannosidase
-
-
-
-
Laman
-
-
-
-
Lysosomal acid alpha-mannosidase
-
-
-
-
p-nitrophenyl-alpha-mannosidase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
alpha-D-mannoside mannohydrolase
Also hydrolyses alpha-D-lyxosides and heptopyranosides with the same configuration at C-2, C-3 and C-4 as mannose.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-42-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-alpha-D-mannobiose + H2O
alpha-D-mannose
show the reaction diagram
-
-
-
?
1,3-alpha-D-mannobiose + H2O
alpha-D-mannose
show the reaction diagram
-
-
-
?
1,6-alpha-D-mannobiose + H2O
alpha-D-mannose
show the reaction diagram
-
-
-
?
alpha3,alpha6-D-mannopentaose + H2O
alpha-D-mannose
show the reaction diagram
-
-
-
?
methyl-O-1,4-alpha-D-mannobiose + H2O
alpha-D-mannose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-mannopyranoside + H2O
4-nitrophenol + alpha-D-mannose
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
potent activator at concentration of 1 mM, 12.5fold activation compared to the apoenzyme
Co2+
8.3fold activation at a concentration of 10 mM, resistance of ManA to heat inactivation is increased by presence of Co2+
Fe2+
4.5fold activation at a concentration of 10 mM
Mn2+
3.8fold activation at a concentration of 10 mM
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
strong inhibitor at concentrations of 1 mM or 10 mM
Cu2+
strong inhibitor at concentrations of 1 mM or 10 mM
EDTA
supplied in equimolar amounts or in excess, neutralizes also effect of resistance of ManA to heat inactivation
Fe3+
strong inhibitor at concentrations of 1 mM or 10 mM
Ni2+
strong inhibitor at concentrations of 1 mM or 10 mM
swainsonine
shows competitive inhibition when p-nitrophenyl-Man is used as a substrate
additional information
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00122
swainsonine
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.6
ManA is equally active with mannobiose substrates, released mannose is quantified, resulting in a specific activity of approximately 6.6 U/mg
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
maximum activity of ManA
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
maximum activity of ManA
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
extremophilic organism belonging to the euryarchaeal phylum
UniProt
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
111500
2 * 111500, gel filtration
120000
small fraction of ManA activity, reflecting single subunits
220000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 111500, gel filtration
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
resistance of ManA to heat inactivation (at 80°C)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
SDS-PAGE, anion-exchange, size exclusion chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Angelov, A.; Putyrski, M.; Liebl, W.
Molecular and biochemical characterization of alpha-glucosidase and alpha-mannosidase and their clustered genes from the thermoacidophilic archaeon Picrophilus torridus
J. Bacteriol.
188
7123-7131
2006
Picrophilus torridus (Q6L2X5), Picrophilus torridus
Manually annotated by BRENDA team