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Information on EC 3.2.1.23 - beta-galactosidase and Organism(s) Felis catus and UniProt Accession O19015

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EC Tree
IUBMB Comments
Some enzymes in this group hydrolyse alpha-L-arabinosides; some animal enzymes also hydrolyse beta-D-fucosides and beta-D-glucosides; cf. EC 3.2.1.108 lactase.
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Select one or more organisms in this record: ?
This record set is specific for:
Felis catus
UNIPROT: O19015
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The taxonomic range for the selected organisms is: Felis catus
The enzyme appears in selected viruses and cellular organisms
Synonyms
beta-galactosidase, beta-gal, beta-d-galactosidase, senescence-associated beta-galactosidase, endo-beta-galactosidase, bglap, sa-beta-gal, acid beta-galactosidase, beta galactosidase, betagal, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acid beta-galactosidase
-
-
-
-
beta-D-galactoside galactohydrolase
-
-
-
-
beta-D-glactanase
-
-
-
-
beta-D-lactosidase
-
-
-
-
beta-galase
-
-
-
-
beta-lactosidase
-
-
-
-
driselase
-
-
-
-
Exo-(1-->4)-beta-D-galactanase
-
-
-
-
exo-beta-(1->3)-D-galactanase
-
-
-
-
galactosidase, beta
-
-
-
-
Hydrolact
-
-
-
-
lactase
-
-
-
-
lactosylceramidase II
-
-
-
-
Lactozym
-
-
-
-
Maxilact
-
-
-
-
Oryzatym
-
-
-
-
p-nitrophenyl beta-galactosidase
-
-
-
-
S 2107
-
-
-
-
SR12 protein
-
-
-
-
Sumiklat
-
-
-
-
Trilactase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
beta-D-galactoside galactohydrolase
Some enzymes in this group hydrolyse alpha-L-arabinosides; some animal enzymes also hydrolyse beta-D-fucosides and beta-D-glucosides; cf. EC 3.2.1.108 lactase.
CAS REGISTRY NUMBER
COMMENTARY hide
9031-11-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl alpha-L-arabinoside + H2O
4-methylumbelliferone + alpha-L-arabinose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
show the reaction diagram
-
-
-
-
?
asialofetuin + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
?
glycolipid GM1 ganglioside + H2O
beta-D-galactose + ?
show the reaction diagram
-
-
-
?
o-nitrophenyl beta-D-fucoside + H2O
o-nitrophenol + beta-D-fucose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-galactoside + H2O
p-nitrophenol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
transfer of galactose from p-nitrophenyl-beta-D-galactoside and ganglioside GM1 to various carbohydrate acceptors
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.16
4-methylumbelliferyl-alpha-L-arabinoside
-
-
0.14
4-methylumbelliferyl-beta-D-galactoside
-
-
1.5
asialofetuin
-
-
-
0.17
ganglioside GM1
-
-
56.9
o-nitrophenyl beta-D-fucoside
-
-
0.62
p-nitrophenyl-beta-D-galactoside
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
recombinant enzyme
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
BGAL_FELCA
669
0
75230
Swiss-Prot
Secretory Pathway (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
94000
x * 94000, SDS-PAGE
115000
-
sucrose density gradient centrifugation
62000
-
2 * 62000, SDS-PAGE of reduced enzyme
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 94000, SDS-PAGE
dimer
-
2 * 62000, SDS-PAGE of reduced enzyme
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, protein concentration 1.2 mg/ml, stable for 8 months
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in CHO-K1 cell
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
design of CHO-K1 cells to produce feline beta-galactosidase for potential use in preclinical trials in a cat model of GM1 gangliosidosis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wallenfels, K.; Malhotra, O.M.
beta-Galactosidase
The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbck, K. , eds. )
4
409-430
1960
Klebsiella aerogenes, Aspergillus flavus, Aspergillus niger, Aspergillus oryzae, Bos taurus, Canis lupus familiaris, Capra hircus, Coffea sp., Oryctolagus cuniculus, Escherichia coli, Felis catus, Helix pomatia, Ovis aries, Homo sapiens, Medicago sativa, Malus sp., Neurospora sp., Prunus dulcis, Prunus armeniaca, Prunus persica, Saccharomyces fragilis, Shigella sonnei, Escherichia coli K 12, Escherichia coli ML 308, Escherichia coli ML 309
-
Manually annotated by BRENDA team
Holmes, E.W.; O'Brien, J.S.
Purification and properties of acid beta-galactosidase from feline liver
Biochemistry
18
952-958
1979
Felis catus
Manually annotated by BRENDA team
Samoylova, T.I.; Martin, D.R.; Morrison, N.E.; Hwang, M.; Cochran, A.M.; Samoylov, A.M.; Baker, H.J.; Cox, N.R.
Generation and characterization of recombinant feline beta-galactosidase for preclinical enzyme replacement therapy studies in GM1 gangliosidosis
Metab. Brain Dis.
23
161-173
2008
Felis catus (O19015), Felis catus
Manually annotated by BRENDA team