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D-arabinan endo alpha-(1,5)-arabinofuranosidase (configuration-retaining)
The enzyme hydrolyses alpha-(1->5)-D-arabinofuranoside bonds in D-arabinan core structure of lipoarabinomannan and arabinogalactan of mycobacterial cell wall. cf. EC 3.2.1.224, D-arabinan exo beta-(1,2)-arabinofuranosidase (non-reducing end) and EC 3.2.1.225, D-arabinan exo alpha-(1,3)/(1,5)-arabinofuranosidase (non-reducing end).
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4-nitrophenyl alpha-D-arabinofuranoside + H2O
4-nitrophenol + alpha-D-arabinofuranose
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Substrates: -
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branched docosasaccharide A22BbetaT + H2O
branched octasaccharide A8Bbeta + D-arabinose
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Substrates: -
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branched docosasaccharide Araf22BT + H2O
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Substrates: -
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D-arabinan + H2O
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Substrates: -
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linear nonasaccharide A8BT + H2O
branched pentasaccharide A5B + D-arabinose
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Substrates: -
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linear nonasaccharide A9LT + H2O
linear tetrasaccharide A4L + D-arabinose
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Substrates: -
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lipoarabinomannan + H2O
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lipoarabinomannan + H2O
mannan core + oligoarabinosides
pilin oligosaccharide + H2O
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Substrates: -
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additional information
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arabinogalactan + H2O

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Substrates: -
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arabinogalactan + H2O
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lipoarabinomannan + H2O

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Substrates: -
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lipoarabinomannan + H2O
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Substrates: -
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lipoarabinomannan + H2O

mannan core + oligoarabinosides
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Substrates: the carboxy terminus of the enzyme mediates chain length extension of the arabinan in lipoarabinomannan
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lipoarabinomannan + H2O
mannan core + oligoarabinosides
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Substrates: the carboxy terminus of the enzyme mediates chain length extension of the arabinan in lipoarabinomannan
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additional information

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Substrates: no activity with D-galactan
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additional information
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Substrates: the alpha-methyl glycoside Me-alpha-A4B is synthesized from branched pentasaccharide A5BT with isoform EndoMA1 by transglycosylation activity in the presence of methanol
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x * 115390, calculated from amino acid sequence
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x * 115390, calculated from amino acid sequence
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homodimer

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2 * 53678, isoform EndoMA1, calculated from amino acid sequence
homodimer
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2 * 52400, isoform EndoMA1, SDS-PAGE
homodimer
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2 * 53678, isoform EndoMA1, calculated from amino acid sequence
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homodimer
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2 * 52400, isoform EndoMA1, SDS-PAGE
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Kotani, S.; Kato, T.; Matsuda, T.; Kato, K.; Misaki, A.
Chemical structure of the antigenic determinants of cell wall polysaccharide of Mycobacterium tuberculosis strain H37Rv
Biken J.
14
379-387
1971
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
brenda
Shi, L.; Berg, S.; Lee, A.; Spencer, J.S.; Zhang, J.; Vissa, V.; McNeil, M.R.; Khoo, K.H.; Chatterjee, D.
The carboxy terminus of EmbC from Mycobacterium smegmatis mediates chain length extension of the arabinan in lipoarabinomannan
J. Biol. Chem.
281
19512-19526
2006
Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc2155
brenda
Al-Jourani, O.; Benedict, S.T.; Ross, J.; Layton, A.J.; van der Peet, P.; Marando, V.M.; Bailey, N.P.; Heunis, T.; Manion, J.; Mensitieri, F.; Franklin, A.; Abellon-Ruiz, J.; Oram, S.L.; Parsons, L.; Cartmell, A.; Wright, G.S.A.; Basle, A.; Trost, M.; Henrissat, B.; Munoz-Munoz, J.; Hirt, R.P.; Kiessling, L.L.; Lovering, A.L.; Williams, S.J.; Lowe, E.C.; Moynihan, P.J.
Identification of D-arabinan-degrading enzymes in mycobacteria
Nat. Commun.
14
2233
2023
Dysgonomonas gadei
brenda
Shimokawa, M.; Ishiwata, A.; Kashima, T.; Nakashima, C.; Li, J.; Fukushima, R.; Sawai, N.; Nakamori, M.; Tanaka, Y.; Kudo, A.; Morikami, S.; Iwanaga, N.; Akai, G.; Shimizu, N.; Arakawa, T.; Yamada, C.; Kitahara, K.; Tanaka, K.; Ito, Y.; Fushinobu, S.; Fujita, K.
Identification and characterization of endo-alpha-, exo-alpha-, and exo-beta-D-arabinofuranosidases degrading lipoarabinomannan and arabinogalactan of mycobacteria
Nat. Commun.
14
5803
2023
Microbacterium arabinogalactanolyticum, Microbacterium arabinogalactanolyticum JCM9171
brenda