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EC Tree
The taxonomic range for the selected organisms is: Rhizopus sp. ACCC 30795 The enzyme appears in selected viruses and cellular organisms
Synonyms
alpha-galactosidase, alpha-galactosidase a, alpha-gal, alpha-gal a, agalsidase alfa, genzyme, alpha-d-galactosidase, a-galactosidase, fabrazyme, alpha-galactosidase i,
more
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alpha-D-galactosidase
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Alpha-D-galactoside galactohydrolase
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alpha-galactosidase A
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alpha-galactoside galactohydrolase
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ceramidase, galactosylgalactosylglucosyl-
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ceramide trihexosidase
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ceramidetrihexosidase
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ceramidetrihexoside-alpha-galactosidase
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trihexosyl ceramide galactosidase
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trihexosylceramide alpha-galactosidase
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trihexosylceramidealpha-galactosidase
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hydrolysis of O-glycosyl bond
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alpha-D-galactoside galactohydrolase
Also hydrolyses alpha-D-fucosides.
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4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
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melibiose + H2O
D-galactose + D-glucose
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raffinose + H2O
sucrose + alpha-D-galactose
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stachyose + 2 H2O
2 alpha-D-galactose + sucrose
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Cr3+
the activity of recombinant Aga-F78 is significantly enhanced in the presence of Cr3+
Fe3+
the activity of recombinant Aga-F78 is significantly enhanced in the presence of Fe3+
Pb2+
the activity of recombinant Aga-F78 is significantly enhanced in the presence of Pb2+
additional information
the addition of other metal ions or chemicals has little or no effect on the activity
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EDTA
the activity of recombinant Aga-F78 is significantly enhanced in the presence of EDTA
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56.61
after 2461.3fold purification, using 4-nitrophenyl alpha-D-galactoside as substrate, at 37°C
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5.4
calculated from amino acid sequence
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UniProt
brenda
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C7SEV1_9FUNG
724
0
82007
TrEMBL
other Location (Reliability: 3 )
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82000
3 * 82000, SDS-PAGE
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homotrimer
3 * 82000, SDS-PAGE
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5 - 10
highly pH stable over the pH range 5.0-10.0
705088
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50 - 60
the recombinant enzyme loses about 60% of the initial activity after incubation at 50°C for 30 min and is completely inactivated at 60°C for 5 min
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the enzyme is protease-resistant, when combined with trypsin, the enzyme retained over 90% degradability to soybean meal
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Ni-NTA column chromatography
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expressed in Escherichia coli BL21(DE3) cells
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Cao, Y.; Wang, Y.; Luo, H.; Shi, P.; Meng, K.; Zhou, Z.; Zhang, Z.; Yao, B.
Molecular cloning and expression of a novel protease-resistant GH-36 alpha-galactosidase from Rhizopus sp. F78 ACCC 30795
J. Microbiol. Biotechnol.
19
1295-1300
2009
Rhizopus sp. ACCC 30795 (C7SEV1)
brenda