We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Wide specificity for beta-D-glucosides. Some examples also hydrolyse one or more of the following: beta-D-galactosides, alpha-L-arabinosides, beta-D-xylosides, beta-D-fucosides.
The taxonomic range for the selected organisms is: Oryza sativa The enzyme appears in selected viruses and cellular organisms
Synonyms
beta-glucosidase, linamarase, beta-glu, emulsin, bglu1, zm-p60.1, bgl1b, cel3a, novozyme 188, t-cell inhibitor,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
aryl-beta-glucosidase
-
-
-
-
beta-1,6-glucosidase
-
-
-
-
beta-D-glucosidase
-
-
-
-
beta-D-glucoside glucohydrolase
-
-
-
-
beta-glucoside hydrolase
-
-
-
-
OsTAGG
-
OsTAGG1 and OsTAGG2, detected by CM-Sepharose chromatography, at least two isoforms in rice panicles
p-nitrophenyl beta-glucosidase
-
-
-
-
tuberonic acid glucoside (TAG)-hydrolyzing beta-glucosidase
-
a specific beta-glucosidase hydrolyzing tuberonic acid beta-D-glucose
tuberonic acid glucoside beta-glucosidase
-
-
additional information
the enzyme belongs to the glycosyl hydrolase family 1
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hydrolysis of O-glycosyl bond
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
beta-D-glucoside glucohydrolase
Wide specificity for beta-D-glucosides. Some examples also hydrolyse one or more of the following: beta-D-galactosides, alpha-L-arabinosides, beta-D-xylosides, beta-D-fucosides.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside + H2O
?
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
-
-
-
?
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
-
-
-
?
cellobiose + H2O
2 D-glucose
-
-
-
?
cellotriose + H2O
D-glucose + cellobiose
-
-
-
?
2-O-beta-D-glucopyranosylsalicylate + H2O
salicylic acid + beta-D-glucose
-
25% relative activity compared to hydrolysis of tuberonic acid beta-D-glucoside
-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
415% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + beta-D-galactose
29.4% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
-
178% relative activity compared to hydrolysis of tuberonic acid beta-D-glucoside
-
-
?
cellobiose [beta-(1-4)-linked] + H2O
?
beta-(1-4)-linked cellobiose
-
-
?
gentiobiose + H2O
?
beta-(1-6)-linked gentiobiose
-
-
?
laminaribiose + H2O
?
beta-(1-3)-linked laminaribose, 55.7% activity compared to n-octyl beta-D-glucopyranoside
-
-
?
laminaritriose + H2O
?
7.43% activity compared to n-octyl beta-D-glucopyranoside
-
-
?
methyl tuberonic acid beta-D-glucoside + H2O
methyl tuberonic acid + beta-D-glucose
-
i.e. 12-hydroxyjasmonic acid beta-d-glucosid methyl ester, 80% relative activity compared to hydrolysis of tuberonic acid beta-D-glucoside
-
-
?
n-heptyl-beta-D-glucoside + H2O
?
29.5% activity compared to n-octyl beta-D-glucopyranoside
-
-
?
n-octyl beta-D-glucopyranoside + H2O
?
100% activity
-
-
?
sophorose + H2O
?
beta-(1-2)-linked sophorose, 31.9% activity compared to n-octyl beta-D-glucopyranoside
-
-
?
tuberonic acid beta-D-glucoside + H2O
tuberonic acid + beta-D-glucose
-
-
-
-
?
additional information
?
-
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
100% activity
-
-
?
additional information
?
-
the enzyme is not able to hydrolyze n-octyl beta-D-thioglucopyranoside, amygdalin, dalcochinin glucoside, gossypin, linamarin, naringin, phlorizin, prunasin, pyridoxine-5-O-beta-D-glucoside, and quercitin 3-beta-D-glucoside. Negligible hydrolysis of 4-nitrophenyl-alpha-L-arabinoside, 4-nitrophenyl-beta-D-cellobioside, 4-nitrophenyl-beta-D-xyloside, 4-nitrophenyl-beta-D-mannoside, 4-nitrophenyl-beta-D-glucuronide, and 4-nitrophenyl-alpha-D-glucoside, cellotriose, cellotetraose, cellopentaose, cellohexaose, laminaritetraose, laminaripentaose, apigenin 7-glucoside, glycitin, diadzin, genistin, esculin, arbutin, coumaryl alcohol beta-D-glucoside, coniferin, and salicin
-
-
?
additional information
?
-
-
the enzyme is not able to hydrolyze n-octyl beta-D-thioglucopyranoside, amygdalin, dalcochinin glucoside, gossypin, linamarin, naringin, phlorizin, prunasin, pyridoxine-5-O-beta-D-glucoside, and quercitin 3-beta-D-glucoside. Negligible hydrolysis of 4-nitrophenyl-alpha-L-arabinoside, 4-nitrophenyl-beta-D-cellobioside, 4-nitrophenyl-beta-D-xyloside, 4-nitrophenyl-beta-D-mannoside, 4-nitrophenyl-beta-D-glucuronide, and 4-nitrophenyl-alpha-D-glucoside, cellotriose, cellotetraose, cellopentaose, cellohexaose, laminaritetraose, laminaripentaose, apigenin 7-glucoside, glycitin, diadzin, genistin, esculin, arbutin, coumaryl alcohol beta-D-glucoside, coniferin, and salicin
-
-
?
additional information
?
-
-
jasmonic acid beta-D-glucosyl ester + H2O, product: jasmonic acid + beta-D-glucose, 51% relative activity compared to hydrolysis of tuberonic acid beta-D-glucoside
-
-
?
additional information
?
-
-
no or negligible hydrolyzing activity with zeatin-O-glucoside, linamarin, or 4-nitrophenyl-beta-D-mannoside, 4-nitrophenyl-beta-D-galactoside, 4-nitrophenyl-beta-D-cellobioside, and 4-nitrophenyl-alpha-D-glucoside
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.139 - 2.1
4-nitrophenyl-beta-D-glucopyranoside
0.5
4-nitrophenyl-beta-D-fucopyranoside
in 100 mM sodium acetate, pH 5.0, at 30°C
6.06
4-nitrophenyl-beta-D-galactopyranoside
in 100 mM sodium acetate, pH 5.0, at 30°C
6.3
4-nitrophenyl-beta-D-glucopyranoside
in 100 mM sodium acetate, pH 5.0, at 30°C
15.3
cellobiose
beta-(1-4)-linked cellobiose,in 100 mM sodium acetate, pH 5.0, at 30°C
14.9
gentiobiose
beta-(1-6)-linked gentiobiose,in 100 mM sodium acetate, pH 5.0, at 30°C
3.6
laminaribiose
beta-(1-3)-linked laminaribose,in 100 mM sodium acetate, pH 5.0, at 30°C
8.7
laminaritriose
in 100 mM sodium acetate, pH 5.0, at 30°C
5
n-heptyl-beta-D-glucoside
in 100 mM sodium acetate, pH 5.0, at 30°C
9.8
sophorose
beta-(1-2)-linked sophorose, in 100 mM sodium acetate, pH 5.0, at 30°C
0.032
tuberonic acid beta-D-glucoside
-
pH 4.0, 37°C
0.139
4-nitrophenyl-beta-D-glucopyranoside
mutant N190H, pH 5.0, 30°C
0.201
4-nitrophenyl-beta-D-glucopyranoside
mutant L442R, pH 5.0, 30°C
0.201
4-nitrophenyl-beta-D-glucopyranoside
wild-type, pH 5.0, 30°C
0.95
4-nitrophenyl-beta-D-glucopyranoside
mutant I179V, pH 5.0, 30°C
2.1
4-nitrophenyl-beta-D-glucopyranoside
mutant N245V, pH 5.0, 30°C
14.3
cellobiose
mutant N190H, pH 5.0, 30°C
21.9
cellobiose
mutant L442R, pH 5.0, 30°C
22
cellobiose
wild-type, pH 5.0, 30°C
61.1
cellobiose
mutant I179V, pH 5.0, 30°C
74.4
cellobiose
mutant N245V, pH 5.0, 30°C
0.16
cellotriose
mutant N190H, pH 5.0, 30°C
0.22
cellotriose
wild-type, pH 5.0, 30°C
0.23
cellotriose
mutant L442R, pH 5.0, 30°C
1.08
cellotriose
mutant I179V, pH 5.0, 30°C
9.25
cellotriose
mutant N245V, pH 5.0, 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4.57 - 6.76
4-nitrophenyl-beta-D-glucopyranoside
33.5
4-nitrophenyl-beta-D-fucopyranoside
in 100 mM sodium acetate, pH 5.0, at 30°C
9.8
4-nitrophenyl-beta-D-galactopyranoside
in 100 mM sodium acetate, pH 5.0, at 30°C
38.9
4-nitrophenyl-beta-D-glucopyranoside
in 100 mM sodium acetate, pH 5.0, at 30°C
0.13
cellobiose
beta-(1-4)-linked cellobiose,in 100 mM sodium acetate, pH 5.0, at 30°C
0.17
gentiobiose
beta-(1-6)-linked gentiobiose, in 100 mM sodium acetate, pH 5.0, at 30°C
6.17
laminaribiose
beta-(1-3)-linked laminaribose,in 100 mM sodium acetate, pH 5.0, at 30°C
1.64
laminaritriose
in 100 mM sodium acetate, pH 5.0, at 30°C
4.25
n-heptyl-beta-D-glucoside
in 100 mM sodium acetate, pH 5.0, at 30°C
9.8
sophorose
beta-(1-2)-linked sophorose, in 100 mM sodium acetate, pH 5.0, at 30°C
additional information
additional information
-
in the presence of 50 mM azide, the apparent kcat values of the E176Q and E176A mutants are nearly independent of the pH from pH 5-10, while those of the wild-type and E176D enzymes decrease 30 and 8fold, respectively, as the pH increases from 5 to 10
-
4.57
4-nitrophenyl-beta-D-glucopyranoside
mutant L442R, pH 5.0, 30°C
4.69
4-nitrophenyl-beta-D-glucopyranoside
wild-type, pH 5.0, 30°C
4.7
4-nitrophenyl-beta-D-glucopyranoside
mutant N245V, pH 5.0, 30°C
6.08
4-nitrophenyl-beta-D-glucopyranoside
mutant I179V, pH 5.0, 30°C
6.76
4-nitrophenyl-beta-D-glucopyranoside
mutant N190H, pH 5.0, 30°C
1.12
cellobiose
mutant L442R, pH 5.0, 30°C
1.16
cellobiose
wild-type, pH 5.0, 30°C
2.09
cellobiose
mutant N190H, pH 5.0, 30°C
3.42
cellobiose
mutant I179V, pH 5.0, 30°C
7.17
cellobiose
mutant N245V, pH 5.0, 30°C
2.35
cellotriose
wild-type, pH 5.0, 30°C
2.58
cellotriose
mutant L442R, pH 5.0, 30°C
3.05
cellotriose
mutant N190H, pH 5.0, 30°C
3.3
cellotriose
mutant I179V, pH 5.0, 30°C
6.58
cellotriose
mutant N245V, pH 5.0, 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
67
4-nitrophenyl-beta-D-fucopyranoside
in 100 mM sodium acetate, pH 5.0, at 30°C
1.6
4-nitrophenyl-beta-D-galactopyranoside
in 100 mM sodium acetate, pH 5.0, at 30°C
6.2
4-nitrophenyl-beta-D-glucopyranoside
in 100 mM sodium acetate, pH 5.0, at 30°C
0.0085
cellobiose
beta-(1-4)-linked cellobiose, in 100 mM sodium acetate, pH 5.0, at 30°C
0.011
gentiobiose
beta-(1-6)-linked gentiobiose, in 100 mM sodium acetate, pH 5.0, at 30°C
1.7
laminaribiose
beta-(1-3)-linked laminaribose, in 100 mM sodium acetate, pH 5.0, at 30°C
0.18
laminaritriose
in 100 mM sodium acetate, pH 5.0, at 30°C
0.85
n-heptyl-beta-D-glucoside
in 100 mM sodium acetate, pH 5.0, at 30°C
0.96
sophorose
beta-(1-2)-linked sophorose, in 100 mM sodium acetate, pH 5.0, at 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
3.73
-
tuberonic acid beta-D-glucoside as substrate, 4300fold purified protein, pH 4.0, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
SwissProt
brenda
subsp. japonica, gene bglu1
SwissProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
during grain-filling
brenda
-
-
brenda
-
purification of OsTAGG1 from immature rice seeds
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
50000
x * 66000, tagged recombinant enzyme, SDS-PAGE, x * 50000, detagged recombinant enzyme, SDS-PAGE
66000
x * 66000, tagged recombinant enzyme, SDS-PAGE, x * 50000, detagged recombinant enzyme, SDS-PAGE
26000
-
1 * 42000 + 1 * 26000, SDS-PAGE
42000
-
1 * 42000 + 1 * 26000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
x * 66000, tagged recombinant enzyme, SDS-PAGE, x * 50000, detagged recombinant enzyme, SDS-PAGE
dimer
-
1 * 42000 + 1 * 26000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
free enzyme and its covalent intermediate with 2-deoxy-2-fluoroglucoside at 2.2 A and 1.55 A resolution, respectively. The complex with the 2-fluoroglucoside includes a glycerol molecule, which may make a nucleophilic attack on the anomeric carbon in a transglycosylation reaction. Sugars are positioned as acceptors for transglycosylation by their interactions with E176, the catalytic acid/base, and Y131. Residues I179, N190 and N245 appear to interact with the substrates
purified recombinant enzyme, free and in a complex with 2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside inhibitor, growth of small crystals in 18% w/v PEG 8000 with 0.1 M sodium cacodylate, pH 6.5, and 0.2 M zinc acetate, 1-2 days, microseeding into 23% w/v PEG MME 5000, 0.2 M ammonium sulfate, and 0.1 M MES, pH 6.7, resulting in larger crystals, 1-2 months, X-ray diffraction structure determination and analysis at 2.15 A and 2.75 A resolution, respectively, molecular replacement, screening and method optimization, overview
thioredoxin/His6-tagged 4BGlu12 fusion protein complexed with 2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside, hanging drop vapor diffusion method, using 19% polyethylene glycol (3350 or 2000) in 0.1 M Tris-HCl pH 8.5, 0.16 M NaCl, at 15°C
in complex with a covalent intermediate, 2-deoxy-2-fluoroglucoside and, n-octyl-beta-D-thioglucopyranoside, microbatch under oil method, using 0.1 M Bis-Tris, pH 6.5, and 20% PEG MME 5000
structures of the BGlu1 E176Q mutant, its complexes with cellotetraose, cellopentaose and laminaribiose, and its covalent intermediate with 2-deoxy-2-fluoroglucoside are determined at 1.65, 1.95, 1.80, 2.80, and 1.90 A resolution, respectively
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
I179V
decrease in catalytic efficiencies
L442R
catalytic efficiencies similar to wild-type
N190H
threefold increase in catalytic efficiency towards cellobiose
N245V
twofold increase in catalytic efficiency towards cellobiose
E176A
-
inactive under standard assay conditions, mutant can be rescued by (small nucleophiles) acetate and azide but not as mutant E176Q
E176D
-
inactive under standard assay conditions, mutant can not be rescued by any small nucleophile
E176Q
-
E176Q active site mutant can be effectively rescued by small nucleophiles, such as acetate, azide and ascorbate, for hydrolysis of aryl glycosides in a pH-independent manner above pH 5. Cellotriose, cellotetraose, cellopentaose, cellohexaose and laminaribiose are not hydrolyzed by the mutant and instead exhibit competitive inhibition
E386D
-
3000fold less active than wild-type
E386Q
-
60000fold less active than wild-type
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2.5 - 7
the activity drops by 50% at pH 2.5 and 6.0, and is negligible from pH 7.0 upward
706319
3.3 - 10.3
-
fairly stable, 90% of the original activity retains after incubation at 4°C for 16 h
710240
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
20 - 40
the enzyme is most stable at 20°C to 30°C, and begins to lose activity after 40 min at 40°C or higher
50
-
after 15 min at various temperatures ranging from 30 to 70°C, the original activity retained up to 50°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
recombinant enzyme from Escherichia coli by immobilized metal-affinity chromatography and removal of the tags, followed by cation exchange chromatography and gel filtration to about 95% purity
Sepharose 6 immobilized metal-ion affinity chromatography on cobalt
ca. 4300fold, yield of 1.1%, ammonium sulfate precipitation, CM-Sepharose, ConA Sepharose, Superdex 200, Resource S, hydroxyapatite
-
immobilized metal (Co2+) affinity chromatography on Talon resin
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli Origami B (DE3) cells
gene bglu1, expression in in Escherichia coli with N-terminal thioredoxin and hexahistidine tags
expressed in Escherichia coli
-
expressed in Escherichia coli Origami (DE3) cells
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Chuenchor, W.; Pengthaisong, S.; Yuvaniyama, J.; Opassiri, R.; Svasti, J.; Ketudat Cairns, J.R.
Purification, crystallization and preliminary X-ray analysis of rice BGlu1 beta-glucosidase with and without 2-deoxy-2-fluoro-beta-D-glucoside
Acta Crystallogr. Sect. F
62
798-801
2006
Oryza sativa (Q75I93), Oryza sativa
brenda
Chuenchor, W.; Pengthaisong, S.; Robinson, R.C.; Yuvaniyama, J.; Oonanant, W.; Bevan, D.R.; Esen, A.; Chen, C.J.; Opassiri, R.; Svasti, J.; Cairns, J.R.
Structural insights into rice BGlu1 beta-glucosidase oligosaccharide hydrolysis and transglycosylation
J. Mol. Biol.
377
1200-1215
2008
Oryza sativa (Q75I93), Oryza sativa
brenda
Sansenya, S.; Cairns, J.R.; Opassiri, R.
Expression, purification, crystallization and preliminary X-ray analysis of rice (Oryza sativa L.) Os4BGlu12 beta-glucosidase
Acta Crystallogr. Sect. F
66
320-323
2010
Oryza sativa (Q75I93), Oryza sativa
brenda
Seshadri, S.; Akiyama, T.; Opassiri, R.; Kuaprasert, B.; Cairns, J.K.
Structural and enzymatic characterization of Os3BGlu6, a rice beta-glucosidase hydrolyzing hydrophobic glycosides and (1->3)- and (1->2)-linked disaccharides
Plant Physiol.
151
47-58
2009
Oryza sativa (Q8L7J2), Oryza sativa
brenda
Wakuta, S.; Hamada, S.; Ito, H.; Matsuura, H.; Nabeta, K.; Matsui, H.
Identification of a beta-glucosidase hydrolyzing tuberonic acid glucoside in rice (Oryza sativa L.)
Phytochemistry
71
1280-1288
2010
Oryza sativa
brenda
Chuenchor, W.; Pengthaisong, S.; Robinson, R.C.; Yuvaniyama, J.; Svasti, J.; Cairns, J.R.
The structural basis of oligosaccharide binding by rice BGlu1 beta-glucosidase
J. Struct. Biol.
173
169-179
2011
Oryza sativa
brenda