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Information on EC 3.2.1.208 - glucosylglycerate hydrolase and Organism(s) Mycolicibacterium hassiacum and UniProt Accession K5BDL0

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IUBMB Comments
The enzyme has been isolated from nontuberculous mycobacteria (e.g. Mycobacterium hassiacum), which accumulate 2-O-(alpha-D-glucopyranosyl)-D-glycerate during growth under nitrogen deprivation.
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This record set is specific for:
Mycolicibacterium hassiacum
UNIPROT: K5BDL0
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The taxonomic range for the selected organisms is: Mycolicibacterium hassiacum
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
slr1670, glucosylglycerate hydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GG hyrolase
-
-
-
-
GGH
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
2-O-(alpha-D-glucopyranosyl)-D-glycerate D-glucohydrolase
The enzyme has been isolated from nontuberculous mycobacteria (e.g. Mycobacterium hassiacum), which accumulate 2-O-(alpha-D-glucopyranosyl)-D-glycerate during growth under nitrogen deprivation.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-O-(alpha-D-glucopyranosyl)-D-glycerate + H2O
D-glucopyranose + D-glycerate
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
5 mM, 111% of initial activity
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8
sodium phosphate buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
nearly undetectable activity below
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.1
calculated from sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 50800, calculated from sequence
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
structure to 2.0 A resolution. Space group P21, four molecules in the asymmetric unit
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
pronounced up-regulation of GgH mRNA in response to the nitrogen depletion
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cereija, T.; Alarico, S.; Empadinhas, N.; Pereira, P.
Production, crystallization and structure determination of a mycobacterial glucosylglycerate hydrolase
Acta Crystallogr. Sect. F
73
536-540
2017
Mycolicibacterium hassiacum (K5BDL0), Mycolicibacterium hassiacum DSM 44199 (K5BDL0)
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Manually annotated by BRENDA team
Alarico, S.; Costa, M.; Sousa, M.; Maranha, A.; Lourenco, E.; Faria, T.; Ventura, M.; Empadinhas, N.
Mycobacterium hassiacum recovers from nitrogen starvation with up-regulation of a novel glucosylglycerate hydrolase and depletion of the accumulated glucosylglycerate
Sci. Rep.
4
6766
2014
Mycolicibacterium hassiacum (K5BDL0), Mycolicibacterium hassiacum, Mycolicibacterium hassiacum DSM 44199 (K5BDL0)
Manually annotated by BRENDA team