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Information on EC 3.2.1.207 - mannosyl-oligosaccharide alpha-1,3-glucosidase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9FN05

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IUBMB Comments
This eukaryotic enzyme cleaves off sequentially the two alpha-1,3-linked glucose residues from the Glc2Man9GlcNAc2 oligosaccharide precursor of immature N-glycosylated proteins.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9FN05
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
prkcsh, ganab, giibeta, er glucosidase ii, alpha glucosidase ii, glucosidase ii beta-subunit, endoplasmic reticulum glucosidase ii, plant glucosidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
plant glucosidase
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alpha-glucosidase II
-
-
-
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ER glucosidase II
-
-
-
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GANAB
-
-
-
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glucosidase II
-
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glucosidase II beta-subunit
-
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GTB1
-
-
-
-
PRKCSH
-
-
-
-
ROT2
-
-
-
-
trimming glucosidase II
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
Glc2Man9GlcNAc2-[protein] 3-alpha-glucohydrolase (configuration-inverting)
This eukaryotic enzyme cleaves off sequentially the two alpha-1,3-linked glucose residues from the Glc2Man9GlcNAc2 oligosaccharide precursor of immature N-glycosylated proteins.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Glc2Man9GlcNAc2 + 2 H2O
Man9GlcNAc2 + 2 D-glucose
show the reaction diagram
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enzyme expression in Schizosaccharomyces pombe mutants either glucosidase II-alpha or both glucosidase II-alpha and -beta minus after cells transformed with the cDNA sequences of Arabidopsis thaliana glucosidase II-alpha encoding gene
-
?
GlcMan9GlcNAc + H2O
Man9GlcNAc + D-glucose
show the reaction diagram
-
enzyme expression in Schizosaccharomyces pombe mutants either glucosidase II-alpha or both glucosidase II-alpha and -beta minus after cells transformed with the cDNA sequences of Arabidopsis thaliana glucosidase II-alpha encoding gene
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
in most species the endoplasmic reticulum localization is mediated by the glucosidase II-beta subunit
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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loss-of-function in the Arabidopsis thaliana glucosidaseII beta-subunit gene confers an polypeptide elf18-insensitive phenotype
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PSL5_ARATH
921
0
104275
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
104000
deduced from cDNA sequences of Arabidopsis thaliana glucosidase II-alpha encoding gene
117000
molecular mass of protein extract of both glucosidase II-alpha and -beta minus Schizosaccharomyces pombe mutant cells after cells transformed with the cDNA sequences of Arabidopsis thaliana glucosidase II-alpha encoding gen and after deglycosylation
119000
western blot analysis of protein extract of both glucosidase II-alpha and -beta minus Schizosaccharomyces pombe mutant cells after cells transformed with the cDNA sequences of Arabidopsis thaliana glucosidase II-alpha encoding gene
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
alphabeta
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
rsw3
Substitution of Ser599 by a Phe residue, inactivation of the alpha-subunit under non-permissive temperature because mutant is strongly temperature-sensitive
additional information
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construction of psl knockout mutants, EFR-mediated signaling is severely impaired in strong subunit beta psl4 mutant plants, and partially and differentially impaired in weak psl5, gIIalpha, mutant plants. EFR signaling is partially and differentially impaired without a significant decrease of the receptor steady-state levels in 2 weakly dysfunctional gIIalpha alleles, designated psl5-1 and rsw3. Phenotypes, overview
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
non-permissive stability
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Schizosaccharomyces pombe mutants either glucosidase II-alpha or both glucosidase II-alpha and -beta minus, vector pTOTO, vector pREP3X, vector pSGP72
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Soussillane, P.; D'Alessio, C.; Paccalet, T.; Fitchette, A.-C.; Parodi, A.J.; Williamson, R.; Plasson, C.; Faye, L.; Gomord, V.
N-glycan trimming by glucosidase II is essential for Arabidopsis development
Glycoconj. J.
26
597-607
2009
Arabidopsis thaliana (Q9FN05)
Manually annotated by BRENDA team
Lu, X.; Tintor, N.; Mentzel, T.; Kombrink, E.; Boller, T.; Robatzek, S.; Schulze-Lefert, P.; Saijo, Y.
Uncoupling of sustained MAMP receptor signaling from early outputs in an Arabidopsis endoplasmic reticulum glucosidase II allele
Proc. Natl. Acad. Sci. USA
106
22522-22527
2009
Arabidopsis thaliana
Manually annotated by BRENDA team
von Numers, N.; Survila, M.; Aalto, M.; Batoux, M.; Heino, P.; Palva, E.T.; Li, J.
Requirement of a homolog of glucosidase II beta-subunit for EFR-mediated defense signaling in Arabidopsis thaliana
Mol. Plant
3
740-750
2010
Arabidopsis thaliana
Manually annotated by BRENDA team