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Information on EC 3.2.1.198 - alpha-mannan endo-1,2-alpha-mannanase

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EC Tree
IUBMB Comments
The enzyme, characterized from the gut bacteria Bacteroides thetaiotaomicron and Bacteroides xylanisolvens, can also catalyse the reaction of EC 3.2.1.130, glycoprotein endo-alpha-1,2-mannosidase.
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This record set is specific for:
UNIPROT: Q8A109
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The expected taxonomic range for this enzyme is: Bacteroides
Reaction Schemes
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Hydrolysis of the terminal alpha-D-mannosyl-(1->3)-alpha-D-mannose disaccharide from alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->2)-alpha-D-mannosyl-(1->2)-alpha-D-mannosyl side chains in fungal cell wall alpha-mannans
Synonyms
BT_3862, BXY_34140, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
alpha-mannan 1,2-[alpha-D-mannosyl-(1->3)-alpha-D-mannose] hydrolase
The enzyme, characterized from the gut bacteria Bacteroides thetaiotaomicron and Bacteroides xylanisolvens, can also catalyse the reaction of EC 3.2.1.130, glycoprotein endo-alpha-1,2-mannosidase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside
show the reaction diagram
-
-
-
?
4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
show the reaction diagram
-
-
-
?
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside + H2O
alpha-1,3-mannobiose + methyl alpha-1,2-mannobioside
show the reaction diagram
-
-
-
?
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranoside + H2O
alpha-1,3-mannobiose + methyl alpha-1,2-mannobioside
show the reaction diagram
-
-
-
?
additional information
?
-
enzyme additionally catalyzes the reaction of EC 3.2.1.130, glycoprotein endo-alpha-1,2-mannosidase. The enzyme shows an approximate 5 kJ/mol preference for mannose-configured substrates in the -2 subsite relative to glucose
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-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-mannopyranosyl-1,3-isofagomine
Kd value 140 nm
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.83
4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
pH 7, 37°C
2.6
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside
pH 7, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0015
4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
pH 7, 37°C
3
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside
pH 7, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00062
4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
pH 7, 37°C
1.15
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside
pH 7, 37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q8A109_BACTN
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)
382
0
43215
TrEMBL
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hakki, Z.; Thompson, A.J.; Bellmaine, S.; Speciale, G.; Davies, G.J.; Williams, S.J.
Structural and kinetic dissection of the endo-alpha-1,2-mannanase activity of bacterial GH99 glycoside hydrolases from Bacteroides spp.
Chemistry
21
1966-1977
2015
Bacteroides thetaiotaomicron (Q8A109), Bacteroides thetaiotaomicron DSM 2079 (Q8A109), Bacteroides xylanisolvens (D6D1V7)
Manually annotated by BRENDA team
Cuskin, F.; Lowe, E.; Temple, M.; Zhu, Y.; Cameron, E.; Pudlo, N.; Porter, N.; Urs, K.; Thompson, A.; Cartmell, A.; Rogowski, A.; Hamilton, B.; Chen, R.; Tolbert, T.; Piens, K.; Bracke, D.; Vervecken, W.; Hakki, Z.; Speciale, G.; Munoz-Munoz, J.; Day, A.;
Human gut Bacteroidetes can utilize yeast mannan through a selfish mechanism
Nature
517
165-169
2015
Bacteroides thetaiotaomicron (Q8A109), Bacteroides thetaiotaomicron DSM 2079 (Q8A109)
Manually annotated by BRENDA team