The 20-O-multi-glycoside ginsenosidase catalyses the hydrolysis of the 20-O-alpha-(1->6)-glycosidic bond and the 20-O-beta-(1->6)-glycosidic bond of protopanaxadiol-type ginsenosides. The enzyme usually leaves a single glucosyl residue attached at position 20, although it can cleave the remaining glucosyl residue with a lower efficiency. Starting with a ginsenoside that is glycosylated at positions 3 and 20, such as ginsenosides Rb1, Rb2, Rb3 and Rc, the most common product is ginsenoside Rd, with a low amount of ginsenoside Rg3 also formed.
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a protopanaxadiol-type ginsenoside with two glycosyl residues at position 20 + H2O = a protopanaxadiol-type ginsenoside with a single glucosyl residue at position 20 + a monosaccharide
The 20-O-multi-glycoside ginsenosidase catalyses the hydrolysis of the 20-O-alpha-(1->6)-glycosidic bond and the 20-O-beta-(1->6)-glycosidic bond of protopanaxadiol-type ginsenosides. The enzyme usually leaves a single glucosyl residue attached at position 20, although it can cleave the remaining glucosyl residue with a lower efficiency. Starting with a ginsenoside that is glycosylated at positions 3 and 20, such as ginsenosides Rb1, Rb2, Rb3 and Rc, the most common product is ginsenoside Rd, with a low amount of ginsenoside Rg3 also formed.
ginsenosidase type V enzymes hydrolyze the sugars linked to C-20 and C-6 in the PPD-type ginsenosides with the hydrolysis patterns of B, E and F. Crude enzyme from Penicillium sp. and beta-glucosidase from Actinosynnema mirum convert ginsenosides Re and Rg1 to Rh1 and APPT, respectively
ginsenosidase type II does not hydrolyze the 3-O-beta-glucoside of Rb1, Rb2, Rb3. Ginsenosidase type II does not hydrolyze the glycosides of protopanaxatriol type ginsenoside such as Re, Rf, Rg1
ginsenosidase type II does not hydrolyze the 3-O-beta-glucoside of Rb1, Rb2, Rb3. Ginsenosidase type II does not hydrolyze the glycosides of protopanaxatriol type ginsenoside such as Re, Rf, Rg1
ginsenosidase type V enzymes hydrolyze the sugars linked to C-20 and C-6 in the PPD-type ginsenosides with the hydrolysis patterns of B, E and F. Crude enzyme from Penicillium sp. and beta-glucosidase from Actinosynnema mirum convert ginsenosides Re and Rg1 to Rh1 and APPT, respectively
ginsenosidase type V enzymes hydrolyze the sugars linked to C-20 and C-6 in the PPD-type ginsenosides with the hydrolysis patterns of B, E and F. Crude enzyme from Penicillium sp. and beta-glucosidase from Actinosynnema mirum convert ginsenosides Re and Rg1 to Rh1 and APPT, respectively
ginsenosidase type V enzymes hydrolyze the sugars linked to C-20 and C-6 in the PPD-type ginsenosides with the hydrolysis patterns of B, E and F. Crude enzyme from Penicillium sp. and beta-glucosidase from Actinosynnema mirum convert ginsenosides Re and Rg1 to Rh1 and APPT, respectively
in a reaction at 85°C and pH 5.0, 25 g/l of ginsenoside Rc is transformed into 21.8 g/l of ginsenoside Rd within 60 min, with a corresponding molar conversion of 99.4% and a high ginsenoside Rd productivity of 21800 mg/l/h
in a reaction at 85°C and pH 5.0, 25 g/l of ginsenoside Rc is transformed into 21.8 g/l of ginsenoside Rd within 60 min, with a corresponding molar conversion of 99.4% and a high ginsenoside Rd productivity of 21800 mg/l/h