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Information on EC 3.2.1.192 - ginsenoside Rb1 beta-glucosidase

for references in articles please use BRENDA:EC3.2.1.192
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IUBMB Comments
Ginsenosidases catalyse the hydrolysis of glycosyl moieties attached to the C-3, C-6 or C-20 position of ginsenosides. They are specific with respect to the nature of the glycosidic linkage, the position and the order in which the linkages are cleaved. Ginsenoside Rb1 beta-glucosidase specifically and sequentially hydrolyses the 20-[beta-D-glucopyranosyl-(1->6)-beta-D glucopyranosyloxy] residues attached to position 20 by first hydrolysing the (1->6)-glucosidic bond to generate ginsenoside Rd as an intermediate, followed by hydrolysis of the remaining 20-O-beta-D-glucosidic bond.
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The expected taxonomic range for this enzyme is: Paecilomyces sp. (in: Eurotiomycetes)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ginsenoside Rb1 + 2 H2O = ginsenoside Rg3 + 2 D-glucopyranose
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ginsenoside Rb1 + H2O = ginsenoside Rd + D-glucopyranose
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ginsenoside Rd + H2O = ginsenoside Rg3 + D-glucopyranose
show the reaction diagram
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PATHWAY SOURCE
PATHWAYS
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