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Information on EC 3.2.1.186 - protodioscin 26-O-beta-D-glucosidase and Organism(s) Hellenia speciosa and UniProt Accession Q42707

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EC Tree
IUBMB Comments
The enzyme has been characterized from the plants Cheilocostus speciosus and Solanum torvum. It also hydrolyses the 26-beta-D-glucose group from related steroid glucosides such as protogracillin, torvoside A and torvoside H.
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This record set is specific for:
Hellenia speciosa
UNIPROT: Q42707
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Word Map
The taxonomic range for the selected organisms is: Hellenia speciosa
The enzyme appears in selected viruses and cellular organisms
Synonyms
sbgl3, torvosidase, csf26g1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
furostanol 26-O-beta-D-glucoside glucohydrolase
-
furostanol glycoside 26-O-beta-D-glucosidase
-
SYSTEMATIC NAME
IUBMB Comments
protodioscin glucohydrolase
The enzyme has been characterized from the plants Cheilocostus speciosus and Solanum torvum. It also hydrolyses the 26-beta-D-glucose group from related steroid glucosides such as protogracillin, torvoside A and torvoside H.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
-
-
-
?
protodioscin + H2O
26-deglucoprotodioscin + D-glucose
show the reaction diagram
protogracillin
26-deglucoprotogracillin + D-glucose
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
the enzyme is involved in the postharvest conversion of furostanol glycosides to spirostanol glycosides in Costus speciosus. Furostanol glycosides are the precursors of spirostane glycosides, containing a glucose unit attached to the C-26 hydroxyl of the aglycone
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta-sitosterol
-
conduritol beta-epoxide
-
D-glucono-1,5-lactone
0.25 mM, 50% inhibition
diosgenin
0.079, 50% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
protogracillin
pH 5.0, 40°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.4
beta-sitosterol
Hellenia speciosa
pH 5.0, 40°C
2.8
cholesterol
Hellenia speciosa
pH 5.0, 40°C
2.4
conduritol beta-epoxide
Hellenia speciosa
pH 5.0, 40°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
325
pH 5.0, 40°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 5.5
substrate: protogracillin
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
F26G_HELSP
562
0
63649
Swiss-Prot
Chloroplast (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
110000
gel filtration
54000
1 * 54000 + 1 * 58000, SDS-PAGE
58000
1 * 54000 + 1 * 58000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
1 * 54000 + 1 * 58000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 20 mM potassium phosphate buffer, pH 7.0, stable for 3 weeks
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Inoue, K.; Ebizuka, Y.
Purification and characterization of furostanol glycoside 26-O-beta-glucosidase from Costus speciosus rhizomes
FEBS Lett.
378
157-160
1996
Hellenia speciosa (Q42707)
Manually annotated by BRENDA team
Inoue, K.; Shibuya, M.; Yamamoto, K.; Ebizuka, Y.
Molecular cloning and bacterial expression of a cDNA encoding furostanol glycoside 26-O-beta-glucosidase of Costus speciosus
FEBS Lett.
389
273-277
1996
Hellenia speciosa (Q42707), Hellenia speciosa
Manually annotated by BRENDA team