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4-methylumbelliferyl-alpha-D-glucoside + H2O
4-methylumbelliferol + D-glucose
Substrates: -
Products: -
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4-nitrophenyl alpha-D-glucopyranoside + H2O
4-nitrophenol + alpha-D-glucose
Substrates: low activity
Products: -
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4-nitrophenyl alpha-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
Substrates: low activity
Products: -
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4-nitrophenyl alpha-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
Substrates: best substrate
Products: -
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4-nitrophenyl alpha-D-xyloside + H2O
4-nitrophenol + alpha-D-xylose
Substrates: -
Products: -
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4-nitrophenyl alpha-maltoside + H2O
4-nitrophenyl alpha-D-glucoside + D-glucose
4-nitrophenyl beta-isoprimeveroside + H2O
4-nitrophenyl beta-D-glucoside + D-xylose
Substrates: -
Products: -
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4-nitrophenyl beta-isoprimeveroside + H2O
alpha-D-xylose + 4-nitrophenyl beta-D-glucopyranoside
4-nitrophenyl-alpha-D-glucoside + H2O
4-nitrophenol + alpha-D-glucose
4-nitrophenyl-alpha-D-xylopyranoside + H2O
4-nitrophenol + alpha-D-xylose
Substrates: -
Products: -
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4-nitrophenyl-alpha-D-xyloside + H2O
4-nitrophenol + alpha-D-xylose
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc + D-xylose
Substrates: low activity
Products: -
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alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc + H2O
?
Substrates: low activity
Products: -
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alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
D-xylose + beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4) D-Glc
-
Substrates: -
Products: -
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alpha-D-xylopyranosyl fluoride + H2O
alpha-D-xylose + fluoride
isoprimeverose + H2O
alpha-D-xylose + beta-D-glucose
isoprimeverose + H2O
D-xylose + D-glucose
Substrates: 6-O-(alpha-D-xylopyranosyl)-D-glucose or 6-O-alpha-D-xylopyranosyl-beta-D-glucopyranose, low activity
Products: -
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maltose + H2O
2 D-glucose
maltotetraose + H2O
maltotriose + D-glucose
Substrates: -
Products: -
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maltotriose + H2O
D-maltose + D-glucose
tamarind seed xyloglucan + H2O
D-xylose + ?
Substrates: -
Products: -
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Xyl-alpha-(1->6)-Glc-beta-(1->4)-Glc + H2O
?
Substrates: -
Products: -
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xyloglucan + H2O
?
-
Substrates: pea xyloglucan, tamarind xyloglucans, etiolated peas, green peas, corn stover, lamb's quarters
Products: -
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xyloglucan + H2O
xylose + ?
xyloglucan oligosaccharide + H2O
?
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Substrates: extensive interactions between the enzyme's active-site variants and xyloglucan hexa- and heptasaccharides. The enzyme recognizes the entire cello-tetraosyl backbone of the substrate and product in positive enzyme subsites and makes further significant interactions with internal pendant alpha-(1->6)-linked xylosyl units, role of the PA14 domain, stereochemistry of the hydrolysis, overview
Products: -
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xyloglucan oligosaccharide + H2O
alpha-D-xylose + ?
additional information
?
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4-nitrophenyl alpha-maltoside + H2O
4-nitrophenyl alpha-D-glucoside + D-glucose
Substrates: low activity
Products: -
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4-nitrophenyl alpha-maltoside + H2O
4-nitrophenyl alpha-D-glucoside + D-glucose
Substrates: low activity
Products: -
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4-nitrophenyl beta-isoprimeveroside + H2O
alpha-D-xylose + 4-nitrophenyl beta-D-glucopyranoside
Substrates: -
Products: -
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4-nitrophenyl beta-isoprimeveroside + H2O
alpha-D-xylose + 4-nitrophenyl beta-D-glucopyranoside
Substrates: -
Products: -
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4-nitrophenyl-alpha-D-glucoside + H2O
4-nitrophenol + alpha-D-glucose
Substrates: kcat/KM is 0.2% compared to the value for 4-nitrophenyl-beta-isoprimeveroside
Products: -
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4-nitrophenyl-alpha-D-glucoside + H2O
4-nitrophenol + alpha-D-glucose
Substrates: kcat/KM is 0.2% compared to the value for 4-nitrophenyl-beta-isoprimeveroside
Products: -
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4-nitrophenyl-alpha-D-xyloside + H2O
4-nitrophenol + alpha-D-xylose
Substrates: -
Products: -
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4-nitrophenyl-alpha-D-xyloside + H2O
4-nitrophenol + alpha-D-xylose
-
Substrates: -
Products: -
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4-nitrophenyl-alpha-D-xyloside + H2O
4-nitrophenol + alpha-D-xylose
Substrates: kcat/KM is 3% compared to the value for 4-nitrophenyl-beta-isoprimeveroside
Products: -
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4-nitrophenyl-alpha-D-xyloside + H2O
4-nitrophenol + alpha-D-xylose
Substrates: kcat/KM is 3% compared to the value for 4-nitrophenyl-beta-isoprimeveroside
Products: -
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alpha-D-xylopyranosyl fluoride + H2O
alpha-D-xylose + fluoride
Substrates: -
Products: -
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alpha-D-xylopyranosyl fluoride + H2O
alpha-D-xylose + fluoride
-
Substrates: -
Products: -
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isoprimeverose + H2O
alpha-D-xylose + beta-D-glucose
Substrates: kcat/KM is 11.5% compared to the value for 4-nitrophenyl-beta-isoprimeveroside
Products: -
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isoprimeverose + H2O
alpha-D-xylose + beta-D-glucose
Substrates: -
Products: -
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isoprimeverose + H2O
alpha-D-xylose + beta-D-glucose
-
Substrates: -
Products: -
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isoprimeverose + H2O
alpha-D-xylose + beta-D-glucose
Substrates: -
Products: -
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isoprimeverose + H2O
alpha-D-xylose + beta-D-glucose
Substrates: kcat/KM is 11.5% compared to the value for 4-nitrophenyl-beta-isoprimeveroside
Products: -
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isoprimeverose + H2O
alpha-D-xylose + beta-D-glucose
Substrates: -
Products: -
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maltose + H2O
2 D-glucose
Substrates: kcat/KM is 1% compared to the value for 4-nitrophenyl-beta-isoprimeveroside
Products: -
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maltose + H2O
2 D-glucose
Substrates: kcat/KM is 1% compared to the value for 4-nitrophenyl-beta-isoprimeveroside
Products: -
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maltose + H2O
2 D-glucose
Substrates: -
Products: -
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maltotriose + H2O
D-maltose + D-glucose
Substrates: kcat/KM is 3% compared to the value for 4-nitrophenyl-beta-isoprimeveroside
Products: -
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maltotriose + H2O
D-maltose + D-glucose
Substrates: -
Products: -
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xyloglucan + H2O
xylose + ?
-
Substrates: xyloglucan oligosaccharide from tamarind seed xyloglucan
Products: -
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xyloglucan + H2O
xylose + ?
Substrates: -
Products: -
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xyloglucan oligosaccharide + H2O
alpha-D-xylose + ?
Substrates: -
Products: -
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xyloglucan oligosaccharide + H2O
alpha-D-xylose + ?
Substrates: -
Products: -
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xyloglucan oligosaccharide + H2O
alpha-D-xylose + ?
Substrates: xyloglucan oligosaccharide from tamarind seed xyloglucan
Products: -
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xyloglucan oligosaccharide + H2O
alpha-D-xylose + ?
Substrates: -
Products: -
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additional information
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-
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Substrates: the enzyme promotes the release of free glucose and xylose from substrates containing alpha-linked xylose, including isoprimeverose, the heptasaccharide subunit of pea xyloglucan, and tamarind xyloglucan
Products: -
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additional information
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Substrates: CjXyl31A exhibits high regiospecificity for the hydrolysis of xylogluco-oligosaccharides, with a particular preference for longer substrates
Products: -
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additional information
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-
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Substrates: CjXyl31A exhibits high regiospecificity for the hydrolysis of xylogluco-oligosaccharides, with a particular preference for longer substrates
Products: -
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additional information
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-
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Substrates: the enzyme CjXyl31A demonstrates an exquisite regioselectivity for the terminal nonreducing-end alpha-(1->6)-xylose of xyloglucan oliogosaccharides
Products: -
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additional information
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Substrates: specific activity of wild-type enzyme with 4-nitrophenyl-alpha-D-glucoside is 96244fold lower compared to specific activity with isoprimeverose
Products: -
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additional information
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-
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Substrates: specific activity of wild-type enzyme with 4-nitrophenyl-alpha-D-glucoside is 96244fold lower compared to specific activity with isoprimeverose
Products: -
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additional information
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Substrates: the enzyme alos shows transglycosylation activity
Products: -
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additional information
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Substrates: the enzyme alos shows transglycosylation activity
Products: -
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additional information
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Substrates: exo-acting enzyme, attacks the non-reducing end of the substrate. No activity is found on isomaltose, trehalose, sucrose, starch, amylose, glycogen, pullulan, xyloglucan, 4-nitrophenyl-alpha-D-galactoside, 4-nitrophenyl-alpha-L-arabinoside, 4-nitrophenyl-alpha-L-rhamnoside, 4-nitrophenyl-alpha-D-mannoside, and 4-nitrophenyl-alpha-L-fucoside
Products: -
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additional information
?
-
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Substrates: exo-acting enzyme, attacks the non-reducing end of the substrate. No activity is found on isomaltose, trehalose, sucrose, starch, amylose, glycogen, pullulan, xyloglucan, 4-nitrophenyl-alpha-D-galactoside, 4-nitrophenyl-alpha-L-arabinoside, 4-nitrophenyl-alpha-L-rhamnoside, 4-nitrophenyl-alpha-D-mannoside, and 4-nitrophenyl-alpha-L-fucoside
Products: -
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additional information
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-
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Substrates: the enzyme has transxylosidic activity and is able to synthesize alpha-xylosides. The attachment of xylose is greatly favored on the oxygen of the C-6 of a free glucose molecule, the 4-nitrophenyl-beta derivative of glucose, and the 4-nitrophenyl derivative of cellobiose
Products: -
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additional information
?
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Substrates: the enzyme has transxylosidic activity and is able to synthesize alpha-xylosides. The attachment of xylose is greatly favored on the oxygen of the C-6 of a free glucose molecule, the 4-nitrophenyl-beta derivative of glucose, and the 4-nitrophenyl derivative of cellobiose
Products: -
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additional information
?
-
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Substrates: the enzyme has transxylosidic activity, forming, in a retaining mode alpha-xylosides. The attachment of xylose is greatly favored on the oxygen of the C-6 of (i) a free glucose molecule, (ii) the 4-nitrophenyl beta derivative of glucose, and (iii) the 4-nitrophenyl beta-derivative of cellobiose. The exo-acting characteristic of this enzyme is also operative in the synthetic mode as is found in the hydrolytic reaction
Products: -
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additional information
?
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Substrates: the enzyme has transxylosidic activity, forming, in a retaining mode alpha-xylosides. The attachment of xylose is greatly favored on the oxygen of the C-6 of (i) a free glucose molecule, (ii) the 4-nitrophenyl beta derivative of glucose, and (iii) the 4-nitrophenyl beta-derivative of cellobiose. The exo-acting characteristic of this enzyme is also operative in the synthetic mode as is found in the hydrolytic reaction
Products: -
?
additional information
?
-
Substrates: exo-acting enzyme, attacks the non-reducing end of the substrate. No activity is found on isomaltose, trehalose, sucrose, starch, amylose, glycogen, pullulan, xyloglucan, 4-nitrophenyl-alpha-D-galactoside, 4-nitrophenyl-alpha-L-arabinoside, 4-nitrophenyl-alpha-L-rhamnoside, 4-nitrophenyl-alpha-D-mannoside, and 4-nitrophenyl-alpha-L-fucoside
Products: -
?
additional information
?
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Substrates: the enzyme has transxylosidic activity, forming, in a retaining mode alpha-xylosides. The attachment of xylose is greatly favored on the oxygen of the C-6 of (i) a free glucose molecule, (ii) the 4-nitrophenyl beta derivative of glucose, and (iii) the 4-nitrophenyl beta-derivative of cellobiose. The exo-acting characteristic of this enzyme is also operative in the synthetic mode as is found in the hydrolytic reaction
Products: -
?
additional information
?
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Substrates: substrate specificty, overview. The enzyme is not active with 0.16% tamarind seed xyloglucan, maltose, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-alpha-L-rhamnopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-beta-L-arabinopyranoside, 4-nitrophenyl-alpha-L-fucopyranoside, 4-nitrophenyl-beta-D-fucopyranoside, 4-nitrophenyl-beta-L-fucopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, and 4-nitrophenyl-beta-D-galactopyranoside
Products: -
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Larsbrink, J.; Izumi, A.; Ibatullin, F.; Nakhai, A.; Gilbert, H.J.; Davies, G.J.; Brumer, H.
Structural and enzymatic characterisation of a glycoside hydrolase family 31 alpha-xylosidase from Cellvibrio japonicus involved in xyloglucan saccharification
Biochem. J.
436
567-580
2011
Cellvibrio japonicus (B3PBD9), Cellvibrio japonicus
brenda
Okuyama, M.; Kaneko, A.; Mori, H.; Chiba, S.; Kimura, A.
Structural elements to convert Escherichia coli alpha-xylosidase (YicI) into alpha-glucosidase
FEBS Lett.
580
2707-2711
2006
Escherichia coli (P31434), Escherichia coli
brenda
Moracci, M.; Cobucci Ponzano, B.; Trincone, A.; Fusco, S.; De Rosa, M.; van Der Oost, J.; Sensen, C.W.; Charlebois, R.L.; Rossi, M.
Identification and molecular characterization of the first alpha-xylosidase from an archaeon
J. Biol. Chem.
275
22082-22089
2000
Saccharolobus solfataricus (Q9P999), Saccharolobus solfataricus, Saccharolobus solfataricus MT4 (Q9P999)
brenda
Lovering, A.L.; Lee, S.S.; Kim, Y.W.; Withers, S.G.; Strynadka, N.C.
Mechanistic and structural analysis of a family 31 alpha-glycosidase and its glycosyl-enzyme intermediate
J. Biol. Chem.
280
2105-2115
2005
Escherichia coli (P31434)
brenda
Iglesias, N.; Abelenda, J.A.; Rodino, M.; Sampedro, J.; Revilla, G.; Zarra, I.
Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana
Plant Cell Physiol.
47
55-63
2006
Arabidopsis thaliana (Q9S7Y7), Arabidopsis thaliana
brenda
Sampedro, J.; Sieiro, C.; Revilla, G.; Gonzalez-Villa, T.; Zarra, I.
Cloning and expression pattern of a gene encoding an alpha-xylosidase active against xyloglucan oligosaccharides from Arabidopsis
Plant Physiol.
126
910-920
2001
Arabidopsis thaliana (Q9S7Y7), Brassica oleracea var. capitata
brenda
Crombie, H.J.; Chengappa, S.; Jarman, C.; Sidebottom, C.; Reid, J.S.
Molecular characterisation of a xyloglucan oligosaccharide-acting alpha-D-xylosidase from nasturtium (Tropaeolum majus L.) cotyledons that resembles plant apoplastic alpha-D-glucosidases
Planta
214
406-413
2002
Tropaeolum majus (Q9ZP04)
brenda
Okuyama, M.; Mori, H.; Chiba, S.; Kimura A.
Overexpression and characterization of two unknown proteins, YicI and YihQ, originated from Escherichia coli
Protein Expr. Purif.
37
170-179
2004
Escherichia coli (P31434), Escherichia coli
brenda
Trincone, A.; Cobucci-Ponzano, B.; Di Lauro, B.; Rossi, M.; Mitsubishi, Y.; Moracci, M.
Enzymatic synthesis and hydrolysis of xylogluco-oligosaccharides using the first archaeal alpha-xylosidase from Sulfolobus solfataricus
Extremophiles
5
277-282
2001
Saccharolobus solfataricus, Saccharolobus solfataricus (Q9P999), Saccharolobus solfataricus P2 (Q9P999)
brenda
Jabbour, D.; Borrusch, M.S.; Banerjee, G.; Walton, J.D.
Enhancement of fermentable sugar yields by alpha-xylosidase supplementation of commercial cellulases
Biotechnol. Biofuels
6
58
2013
Aspergillus niger
brenda
Silipo, A.; Larsbrink, J.; Marchetti, R.; Lanzetta, R.; Brumer, H.; Molinaro, A.
NMR spectroscopic analysis reveals extensive binding interactions of complex xyloglucan oligosaccharides with the Cellvibrio japonicus glycoside hydrolase family 31 alpha-xylosidase
Chemistry
18
13395-13404
2012
Cellvibrio japonicus
brenda
Matsuzawa, T.; Kimura, N.; Suenaga, H.; Yaoi, K.
Screening, identification, and characterization of alpha-xylosidase from a soil metagenome
J. Biosci. Bioeng.
122
393-399
2016
uncultured microorganism (A0A169PW02)
brenda