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Information on EC 3.2.1.169 - protein O-GlcNAcase
for references in articles please use BRENDA:EC3.2.1.169
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EC Tree 3 Hydrolases
3.2 Glycosylases
3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
3.2.1.169 protein O-GlcNAcase
IUBMB Comments
Within higher eukaryotes post-translational modification of protein serines/threonines with N-acetylglucosamine (O-GlcNAc) is dynamic, inducible and abundant, regulating many cellular processes by interfering with protein phosphorylation. EC 2.4.1.255 (protein O-GlcNAc transferase) transfers GlcNAc onto substrate proteins and EC 3.2.1.169 (protein O-GlcNAcase) cleaves GlcNAc from the modified proteins.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
- 3.2.1.169
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o-glcnacylation
- streptozotocin
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o-glycosylation
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nucleocytoplasmic
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beta-cell
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diabetogenic
- perfringens
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thiamet
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pugnacity
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tetratricopeptide
- thetaiotaomicron
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substrate-assisted
- hexosaminidases
- medicine
Reaction Schemes
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Synonyms
o-glcnac hydrolase, o-glcnac-selective n-acetyl-beta-d-glucosaminidase, btgh84, glycoside hydrolase o-glcnacase, cpoga, cytoplasmic o-glcnacase, endos-like endoglycosidase, cpnagj, o-glcnac-selective-n-acetyl-beta-d-glucosaminidase, neutral o-glcnacase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
beta-N-acetylglucosaminidase
BtGH84
CP40
endo-beta-N-acetylglucosaminidase
EndoS-like endoglycosidase
NCOAT
bifunctional nucleo-cytoplasmic protein with both O-GlcNAcase and histone acetyltransferase domains
O-GlcNAc hydrolase
O-GlcNAcase
OGA
O-GlcNAcase
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O-GlcNAcase
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two forms of O-GlcNAcase have been studied, a full-length isoform that is better characterized, and a shorter nuclear-localized variant, arising from failure to splice out one intron
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O = [protein]-L-serine + N-acetyl-D-glucosamine
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[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-theronine + H2O = [protein]-L-threonine + N-acetyl-D-glucosamine
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PATHWAY SOURCE
PATHWAYS
MetaCyc
protein O-[N-acetyl]-glucosylation
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SYSTEMATIC NAME
IUBMB Comments
[protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine/threonine N-acetylglucosaminyl hydrolase
Within higher eukaryotes post-translational modification of protein serines/threonines with N-acetylglucosamine (O-GlcNAc) is dynamic, inducible and abundant, regulating many cellular processes by interfering with protein phosphorylation. EC 2.4.1.255 (protein O-GlcNAc transferase) transfers GlcNAc onto substrate proteins and EC 3.2.1.169 (protein O-GlcNAcase) cleaves GlcNAc from the modified proteins.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-acetamido-2-deoxy-5-fluoro-beta-D-glucopyranosyl fluoride + H2O
fluoride + N-acetyl-beta-D-glucosaminide
Substrates: a substrate contains a modest leaving group as well as a poor nucleophile, the catalytic efficiency of the enzyme is synergistically impaired. Testing a wide range of such substrates and obtained co-crystals of BtGH84, to test if under the right conditions, such a substrate might be trapped in the active site unhydrolyzed
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3,4,6-triacetyl-O-GlcNAc-Fmoc-Ser-OH + H2O
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3,4,6-triacetyl-O-GlcNAc-Fmoc-Thr-OH + H2O
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3,4-difluorophenyl 2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranose + 3,4-difluorophenol
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3,4-difluorophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranose + 3,4-difluorophenol
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3,4-difluorophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranose + 3,4-difluorophenol
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3,4-difluorophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranose + 3,4-difluorophenol
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3,4-difluorophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranose + 3,4-difluorophenol
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3,4-difluorophenyl 2-deoxy-2-difluoroacetamido-beta-D-glucopyranoside + H2O
3,4-difluorophenol + 2-deoxy-2-difluoroacetamido-beta-D-glucopyranose
Substrates: a substrate contains a modest leaving group as well as a poor nucleophile, the catalytic efficiency of the enzyme is synergistically impaired. Testing a wide range of such substrates and obtained co-crystals of BtGH84, to test if under the right conditions, such a substrate might be trapped in the active site unhydrolyzed
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3,4-dinitrophenyl 2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranose + 3,4-dinitrophenol
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3,4-dinitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranose + 3,4-dinitrophenol
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3,4-dinitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranose + 3,4-dinitrophenol
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3,4-dinitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranose + 3,4-dinitrophenol
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3,4-dinitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranose + 3,4-dinitrophenol
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3,4-dinitrophenyl N-acetyl-beta-D-glucosaminide + H2O
3,4-dinitrophenol + N-acetyl-beta-D-glucosamine
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3-fluoro-4-nitrophenyl 2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranose + 3-fluoro-4-nitrophenol
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3-fluoro-4-nitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranose + 3-fluoro-4-nitrophenol
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3-fluoro-4-nitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranose + 3-fluoro-4-nitrophenol
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3-fluoro-4-nitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranose + 3-fluoro-4-nitrophenol
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3-fluoro-4-nitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranose + 3-fluoro-4-nitrophenol
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3-fluoro-4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
3-fluoro-4-nitrophenol + N-acetyl-beta-D-glucosamine
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3-nitrophenyl 2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranose + 3-nitrophenol
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3-nitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranose + 3-nitrophenol
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3-nitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranose + 3-nitrophenol
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3-nitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranose + 3-nitrophenol
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3-nitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranose + 3-nitrophenol
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3-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
3-nitrophenol + N-acetyl-beta-D-glucosamine
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4-aminophenyl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside + H2O
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Substrates: 67% relative activity compared to 4-nitrophenyl-beta-D-GlcNac
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4-chloro-3-nitrophenyl 2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranose + 4-chloro-3-nitrophenol
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4-chloro-3-nitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranose + 4-chloro-3-nitrophenol
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4-chloro-3-nitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranose + 4-chloro-3-nitrophenol
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4-chloro-3-nitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranose + 4-chloro-3-nitrophenol
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4-chloro-3-nitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranose + 4-chloro-3-nitrophenol
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4-chlorophenyl 2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranose + 4-chlorophenol
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4-chlorophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranose + 4-chlorophenol
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4-chlorophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranose + 4-chlorophenol
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4-chlorophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranose + 4-chlorophenol
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4-chlorophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranose + 4-chlorophenol
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4-chlorophenyl N-acetyl-beta-D-glucosaminide + H2O
4-chlorophenol + N-acetyl-beta-D-glucosamine
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4-cyanophenyl 2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranose + 4-cyanophenol
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4-cyanophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranose + 4-cyanophenol
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4-cyanophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranose + 4-cyanophenol
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4-cyanophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranose + 4-cyanophenol
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4-cyanophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranose + 4-cyanophenol
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4-methoxyphenyl 2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranoside + 4-methoxyphenol
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4-methoxyphenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranose + 4-methoxyphenol
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4-methoxyphenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranose + 4-methoxyphenol
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4-methoxyphenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranose + 4-methoxyphenol
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4-methoxyphenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranose + 4-methoxyphenol
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4-methylumbelliferyl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside + H2O
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Substrates: 33% relative activity compared to 4-nitrophenyl-beta-D-GlcNac
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4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranose
Substrates: substrate with a 4-methylumbelliferone leaving group. A substrate contains a modest leaving group as well as a poor nucleophile, the catalytic efficiency of the enzyme is synergistically impaired. Testing a wide range of such substrates and obtained co-crystals of BtGH84, to test if under the right conditions, such a substrate might be trapped in the active site unhydrolyzed
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4-methylumbelliferyl 2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranose + 4-methylumbelliferone
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4-methylumbelliferyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranose + 4-methylumbelliferone
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4-methylumbelliferyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranose + 4-methylumbelliferone
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4-methylumbelliferyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranose + 4-methylumbelliferone
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4-methylumbelliferyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranose + 4-methylumbelliferone
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4-methylumbelliferyl beta-D-N-acetylglucosaminide + H2O
4-methylumbelliferone + beta-D-N-acetylglucosamine
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-acetyl-beta-D-glucosamine
4-methylumbelliferyl N-difluoroacetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-difluoroacetyl-beta-D-glucosamine
4-methylumbelliferyl N-fluoroacetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-fluoroacetyl-beta-D-glucosamine
4-methylumbelliferyl N-trifluoroacetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-trifluoroacetyl-beta-D-glucosamine
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
4-methylumbelliferol + N-acetyl-beta-D-glucosamine
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4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-acetyl-beta-D-glucosamine
Substrates: fluorogenic substrate, 4MU-NAG
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4-nitrophenyl 2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranose + 4-nitrophenol
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4-nitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranose + 4-nitrophenol
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4-nitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranose + 4-nitrophenol
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4-nitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranose + 4-nitrophenol
-
Substrates: -
Products: -
Products: -
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4-nitrophenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranose + 4-nitrophenol
-
Substrates: -
Products: -
Products: -
?
4-nitrophenyl beta-D-GlcNAc + H2O
4-nitrophenol + N-acetyl-D-glucosamine
-
Substrates: -
Products: -
Products: -
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4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
4-nitrophenyl N-acetyl-beta-D-thioglucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-thioglucosamine
Ala-Ser(O-GlcNAc)-Tyr + H2O
Ala-Ser-Tyr + N-acetyl-beta-D-glucosamine
-
Substrates: is synthesized using standard N-(9-fluorenyl)methoxycarbonyl (FMOC) chemistry
Products: -
Products: -
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fluorescein di(N-acetyl-beta-D-glucosaminide) + H2O
fluorescein mono(N-acetyl-beta-D-glucosaminide) + N-acetyl-D-glucosamine
GlcNAc beta(1-4)GlcNAc + H2O
N-acetyl-D-glucosamine + N-acetyl-D-glucosamine
-
Substrates: 4% relative activity. Enzyme can cleave terminal Glc-NAc from disaccharides to a limited extent as long as GlcNAc is linked to the other saccharides beta-linkage at the C-1 position
Products: -
Products: -
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GlcNAc beta(1-6)Gal + H2O
N-acetyl-D-glucosamine + beta-D-galactose
-
Substrates: 29% relative activity. Enzyme can cleave terminal Glc-NAc from disaccharides to a limited extent as long as GlcNAc is linked to the other saccharides beta-linkage at the C-1 position
Products: -
Products: -
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GlcNAcbeta(1-6)Man-beta-1-OMe + H2O
N-acetyl-D-glucosamine + methyl beta-D-mannoside
-
Substrates: 33% relative activity. Enzyme can cleave terminal Glc-NAc from disaccharides to a limited extent as long as GlcNAc is linked to the other saccharides beta-linkage at the C-1 position
Products: -
Products: -
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methyl 2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranose + methanol
-
Substrates: -
Products: -
Products: -
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methyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranose + methanol
-
Substrates: -
Products: -
Products: -
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methyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranose + methanol
-
Substrates: -
Products: -
Products: -
?
methyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranose + methanol
-
Substrates: -
Products: -
Products: -
?
methyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranose + methanol
-
Substrates: -
Products: -
Products: -
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N-acetyl-4-aminophenyl N-acetyl-beta-D-glucosaminide + H2O
N-acetyl-4-aminophenol + N-acetyl-beta-D-glucosamine
-
Substrates: -
Products: -
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P-(GlcNAc)STST + H2O
PSTST + N-acetyl-D-glucosamine
-
Substrates: -
Products: -
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phenyl 2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-N,O3,O4,O6-tetraacetyl-beta-D-glucopyranose + phenol
-
Substrates: -
Products: -
Products: -
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phenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-butanoyl-beta-D-glucopyranose + phenol
-
Substrates: -
Products: -
Products: -
?
phenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-difluoroacetyl-beta-D-glucopyranose + phenol
-
Substrates: -
Products: -
Products: -
?
phenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-fluoroacetyl-beta-D-glucopyranose + phenol
-
Substrates: -
Products: -
Products: -
?
phenyl 2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranoside + H2O
2-deoxy-2-amino-O3,O4,O6-triacetyl-N-trifluoroacetyl-beta-D-glucopyranose + phenol
-
Substrates: -
Products: -
Products: -
?
phenyl N-acetyl-beta-D-glucosaminide + H2O
phenol + N-acetyl-beta-D-glucosamine
-
Substrates: -
Products: -
Products: -
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VPST(GlcNAc)MSAN + H2O
VPSTMSAN + N-acetyl-beta-D-glucosamine
-
Substrates: -
Products: -
Products: -
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YSD-(GlcNAc)SPSTST + H2O
YSDSPSTST + N-acetyl-D-glucosamine
-
Substrates: -
Products: -
Products: -
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[alpha-crystallin B chain]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[alpha-crystallin B chain]-L-serine + N-acetyl-D-glucosamine
Substrates: the sequence of alpha-crystallin B chain is FPTSTSLSPFYLR
Products: -
Products: -
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[dHCF peptide]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
[dHCF peptide]-L-threonine + N-acetyl-D-glucosamine
-
Substrates: i.e. VPSgTMSAN
Products: -
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[ELK1 protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
[ELK1 protein]-L-threonine + N-acetyl-D-glucosamine
Substrates: the sequence of ELK1 protein is FWSTLSPI
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[host cell factor protein]-3-O-(N-acetyl-beta-D-hexosaminyl)-L-threonine + H2O
[host cell factor protein]-L-threonine + N-acetyl-D-hexosamine
-
Substrates: the sequence of host cell factor protein is IVPSVtASHSLR, t indicates the HexNAc-modified residue
Products: -
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[lamin B1]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
[lamin B1]-L-threonine + N-acetyl-D-glucosamine
Substrates: the sequence of lamin B1 protein is KLSPSPSSRVTVS
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[nucleoporin 153]-3-O-(N-acetyl-beta-D-hexosaminyl)-L-serine + H2O
[nucleoporin 153]-L-serine + N-acetyl-D-hexosamine
-
Substrates: the seque4nce of nucleoporin 153 is FDLIIKPTVPVVsKPSTTDPIQSSK. s indicates the HexNAc-modified residue
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[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[protein]-L-serine + N-acetyl-D-glucosamine
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
[protein]-L-threonine + N-acetyl-D-glucosamine
[TAB1 protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[TAB1 protein]-L-serine + N-acetyl-D-glucosamine
4-methylumbelliferyl beta-D-N-acetylglucosaminide + H2O
4-methylumbelliferone + beta-D-N-acetylglucosamine
Substrates: -
Products: -
Products: -
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4-methylumbelliferyl beta-D-N-acetylglucosaminide + H2O
4-methylumbelliferone + beta-D-N-acetylglucosamine
Substrates: -
Products: -
Products: -
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4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-acetyl-beta-D-glucosamine
Substrates: -
Products: -
Products: -
?
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-acetyl-beta-D-glucosamine
Substrates: -
Products: -
Products: -
?
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-acetyl-beta-D-glucosamine
-
Substrates: -
Products: -
Products: -
?
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-acetyl-beta-D-glucosamine
-
Substrates: -
Products: -
Products: -
?
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-acetyl-beta-D-glucosamine
Substrates: -
Products: -
Products: -
?
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-acetyl-beta-D-glucosamine
-
Substrates: fluorogenic substrate, 4MU-NAG
Products: -
Products: -
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4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-acetyl-beta-D-glucosamine
-
Substrates: MUGlcNAc
Products: -
Products: -
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4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-acetyl-beta-D-glucosamine
Substrates: -
Products: -
Products: -
?
4-methylumbelliferyl N-difluoroacetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-difluoroacetyl-beta-D-glucosamine
Substrates: -
Products: -
Products: -
?
4-methylumbelliferyl N-difluoroacetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-difluoroacetyl-beta-D-glucosamine
-
Substrates: -
Products: -
Products: -
?
4-methylumbelliferyl N-fluoroacetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-fluoroacetyl-beta-D-glucosamine
Substrates: -
Products: -
Products: -
?
4-methylumbelliferyl N-fluoroacetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-fluoroacetyl-beta-D-glucosamine
-
Substrates: -
Products: -
Products: -
?
4-methylumbelliferyl N-trifluoroacetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-trifluoroacetyl-beta-D-glucosamine
Substrates: -
Products: -
Products: -
?
4-methylumbelliferyl N-trifluoroacetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-trifluoroacetyl-beta-D-glucosamine
-
Substrates: -
Products: -
Products: -
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
Substrates: -
Products: -
Products: -
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4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
Substrates: -
Products: -
Products: -
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4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
Substrates: -
Products: -
Products: -
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
Substrates: -
Products: -
Products: -
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4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
Substrates: -
Products: -
Products: -
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4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
Substrates: -
Products: -
Products: -
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4-nitrophenyl N-acetyl-beta-D-thioglucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-thioglucosamine
Substrates: -
Products: -
Products: -
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4-nitrophenyl N-acetyl-beta-D-thioglucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-thioglucosamine
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Substrates: enzyme cleaves p-nitrophenyl-S-GlcNAc with comparable efficiency as p-nitrophenyl-O-GlcNAc
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fluorescein di(N-acetyl-beta-D-glucosaminide) + H2O
fluorescein mono(N-acetyl-beta-D-glucosaminide) + N-acetyl-D-glucosamine
-
Substrates: -
Products: -
Products: -
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fluorescein di(N-acetyl-beta-D-glucosaminide) + H2O
fluorescein mono(N-acetyl-beta-D-glucosaminide) + N-acetyl-D-glucosamine
-
Substrates: FDGlcNAc, fluorogenic substrate
Products: -
Products: -
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fluorescein di(N-acetyl-beta-D-glucosaminide) + H2O
fluorescein mono(N-acetyl-beta-D-glucosaminide) + N-acetyl-D-glucosamine
Substrates: -
Products: -
Products: -
?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[protein]-L-serine + N-acetyl-D-glucosamine
-
Substrates: -
Products: -
Products: -
?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[protein]-L-serine + N-acetyl-D-glucosamine
Substrates: -
Products: -
Products: -
?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[protein]-L-serine + N-acetyl-D-glucosamine
-
Substrates: -
Products: -
Products: -
?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[protein]-L-serine + N-acetyl-D-glucosamine
Substrates: -
Products: -
Products: -
?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[protein]-L-serine + N-acetyl-D-glucosamine
-
Substrates: -
Products: -
Products: -
?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[protein]-L-serine + N-acetyl-D-glucosamine
Substrates: -
Products: -
Products: -
?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
[protein]-L-threonine + N-acetyl-D-glucosamine
-
Substrates: -
Products: -
Products: -
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[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
[protein]-L-threonine + N-acetyl-D-glucosamine
Substrates: -
Products: -
Products: -
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[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
[protein]-L-threonine + N-acetyl-D-glucosamine
-
Substrates: -
Products: -
Products: -
?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
[protein]-L-threonine + N-acetyl-D-glucosamine
Substrates: -
Products: -
Products: -
?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
[protein]-L-threonine + N-acetyl-D-glucosamine
-
Substrates: -
Products: -
Products: -
?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
[protein]-L-threonine + N-acetyl-D-glucosamine
Substrates: -
Products: -
Products: -
?
[TAB1 protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[TAB1 protein]-L-serine + N-acetyl-D-glucosamine
-
Substrates: -
Products: -
Products: -
?
[TAB1 protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[TAB1 protein]-L-serine + N-acetyl-D-glucosamine
-
Substrates: -
Products: -
Products: -
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[TAB1 protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[TAB1 protein]-L-serine + N-acetyl-D-glucosamine
Substrates: the sequence of TAB1 protein is VPYSSAQS
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additional information
?
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Substrates: enzyme cleaves 4-nitrophenyl N-acetyl-beta-D-glucosaminide from modified proteins and also cleaves 4-nitrophenyl N-acetyl-beta-D-thioglucosaminide
Products: -
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additional information
?
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Substrates: when a substrate contains a modest leaving group as well as a poor nucleophile, the catalytic efficiency of the enzyme is synergistically impaired
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additional information
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Substrates: enzyme that removes O-GlcNAc from O-GlcNAcylated proteins
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additional information
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Substrates: has O-GlcNAcase activity against O-GlcNAcylated human proteins, suggesting that the enzyme is a suitable model for further studies into the function of human O-GlcNAcase
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additional information
?
-
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Substrates: the enzyme can hydrolyze all biantennary glycoforms from human and ovine immunglobuline G
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additional information
?
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Substrates: the enzyme cannot hydrolyze bisecting N-acetyl-beta-D-glucosaminide and shows no activity on bovine or caprine immunoglobuline G and 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
Products: -
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additional information
?
-
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Substrates: the enzyme can hydrolyze all biantennary glycoforms from human and ovine immunglobuline G
Products: -
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additional information
?
-
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Substrates: the enzyme cannot hydrolyze bisecting N-acetyl-beta-D-glucosaminide and shows no activity on bovine or caprine immunoglobuline G and 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
Products: -
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additional information
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-
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Substrates: catalyzes the cleavage of beta-O-linked N-acetyl-beta-D-glucosaminide from modified proteins and is a member of the family 84 glycoside hydrolases
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additional information
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-
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Substrates: catalyzing the hydrolytic cleavage of beta-O-linked 2-acetamido-2-deoxy-D-glycopyranose (O-GlcNAc) from serine and threonine residues of posttranslationally modified proteins
Products: -
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additional information
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Substrates: enzyme is responsible for removal of the O-GlcNAc post-translational modification, is being increasingly used to aid in discerning the roles played by this form of intracellular glycosylation
Products: -
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additional information
?
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Substrates: enzyme removes N-acetyl-beta-D-glucosaminide from proteins
Products: -
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additional information
?
-
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Substrates: enzyme that removes N-acetyl-beta-D-glucosamine from O-GlcNAcylated proteins
Products: -
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additional information
?
-
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Substrates: O-GlcNAcase uses a catalytic mechanism involving substrate-assisted catalysis. In this mechanism, the leaving group is expelled from the anomeric center by nucleophilic attack of the 2-acetamido group of the substrate to form an oxazoline intermediate
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additional information
?
-
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Substrates: fluorescein di-N-acetyl-beta-D-galactosaminide (FDGalNAc) is not cleaved by these enzymes, the O-GlcNAcase has substrate specificity toward O-GlcNAc but not O-GalNAc
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additional information
?
-
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Substrates: removes O-N-acetyl-glucosamine residues from O-GlcNAcylated proteins
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additional information
?
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Substrates: strong selectivity for O-GlcNAc-synthetic glycopeptides. Enzyme does not cleave 4-nitrophenylGalNAc and is highly selective for O-GlcNAc bearing glycopeptides. O-GlcNAcase is involved in the regulated removal of O-GlcNAc from O-GlcNAc-bearing glycoproteins in the nucleoplasmic and cytoplasmic compartments of cells
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additional information
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Substrates: shows no activity against other monosaccharides either in alpha or beta linkage to 4-nitrophenyl, such as, 4-nitrophenyl-alpha-D-GlcNAc, 4-nitrophenyl-alpha-D-glucose, 4-nitrophenyl-beta-D-glucose, 4-nitrophenyl-alpha-D-GalNAc, 4-nitrophenyl-beta-D-GalNAc, 4-nitrophenyl-alpha-D-galactose, 4-nitrophenyl-beta-D-galactose, 4-nitrophenyl-alpha-D-mannose, 4-nitrophenyl-alpha-L-fructose
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additional information
?
-
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Substrates: substitution of GlcNAc at other positions prevents cleavage, for example, Gal(1-3)GlcNAc-p-nitrophenol, Gal(1-4)GlcNAc, Gal(1-3)GlcNAc, and UDP-GlcNAc, the enzyme specifically cleave GlcNAc at C-1 position in beta-linkage to other groups
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[protein]-L-serine + N-acetyl-D-glucosamine
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
[protein]-L-threonine + N-acetyl-D-glucosamine
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[protein]-L-serine + N-acetyl-D-glucosamine
-
Substrates: -
Products: -
Products: -
?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[protein]-L-serine + N-acetyl-D-glucosamine
Substrates: -
Products: -
Products: -
?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[protein]-L-serine + N-acetyl-D-glucosamine
-
Substrates: -
Products: -
Products: -
?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[protein]-L-serine + N-acetyl-D-glucosamine
Substrates: -
Products: -
Products: -
?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[protein]-L-serine + N-acetyl-D-glucosamine
-
Substrates: -
Products: -
Products: -
?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O
[protein]-L-serine + N-acetyl-D-glucosamine
Substrates: -
Products: -
Products: -
?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
[protein]-L-threonine + N-acetyl-D-glucosamine
-
Substrates: -
Products: -
Products: -
?
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
[protein]-L-threonine + N-acetyl-D-glucosamine
Substrates: -
Products: -
Products: -
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[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
[protein]-L-threonine + N-acetyl-D-glucosamine
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[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
[protein]-L-threonine + N-acetyl-D-glucosamine
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[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
[protein]-L-threonine + N-acetyl-D-glucosamine
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[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O
[protein]-L-threonine + N-acetyl-D-glucosamine
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Substrates: enzyme cleaves 4-nitrophenyl N-acetyl-beta-D-glucosaminide from modified proteins and also cleaves 4-nitrophenyl N-acetyl-beta-D-thioglucosaminide
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Substrates: when a substrate contains a modest leaving group as well as a poor nucleophile, the catalytic efficiency of the enzyme is synergistically impaired
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Substrates: enzyme that removes O-GlcNAc from O-GlcNAcylated proteins
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Substrates: has O-GlcNAcase activity against O-GlcNAcylated human proteins, suggesting that the enzyme is a suitable model for further studies into the function of human O-GlcNAcase
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Substrates: catalyzes the cleavage of beta-O-linked N-acetyl-beta-D-glucosaminide from modified proteins and is a member of the family 84 glycoside hydrolases
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Substrates: catalyzing the hydrolytic cleavage of beta-O-linked 2-acetamido-2-deoxy-D-glycopyranose (O-GlcNAc) from serine and threonine residues of posttranslationally modified proteins
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Substrates: enzyme is responsible for removal of the O-GlcNAc post-translational modification, is being increasingly used to aid in discerning the roles played by this form of intracellular glycosylation
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Substrates: enzyme removes N-acetyl-beta-D-glucosaminide from proteins
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Substrates: enzyme that removes N-acetyl-beta-D-glucosamine from O-GlcNAcylated proteins
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Substrates: O-GlcNAcase uses a catalytic mechanism involving substrate-assisted catalysis. In this mechanism, the leaving group is expelled from the anomeric center by nucleophilic attack of the 2-acetamido group of the substrate to form an oxazoline intermediate
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Substrates: removes O-N-acetyl-glucosamine residues from O-GlcNAcylated proteins
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Substrates: strong selectivity for O-GlcNAc-synthetic glycopeptides. Enzyme does not cleave 4-nitrophenylGalNAc and is highly selective for O-GlcNAc bearing glycopeptides. O-GlcNAcase is involved in the regulated removal of O-GlcNAc from O-GlcNAc-bearing glycoproteins in the nucleoplasmic and cytoplasmic compartments of cells
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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EDTA, Mg2+, Fe3+, Ca2+, Mn2+, and Co2+ do not affect the activity
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(3aS,5R,6S,7R,7aR)-5-(hydroxymethyl)-2-methyl-5,6,7,7a-tetrahydro-3aH-pyrano[3,2-d][1,3]thiazole-6,7-diol
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NAG-thiazoline, inhibits also other functionally related enzymes
(3aS,5R,6S,7R,7aR)-5-(hydroxymethyl)-2-propyl-5,6,7,7a-tetrahydro-3aH-pyrano[3,2-d][1,3]thiazole-6,7-diol
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NButGT, selective O-GlcNAcase inhibitor
(6S,7R,8R)-8-acetamido-6,7-dihydroxy-5-(hydroxymethyl)-N-phenyl-5,6,7,8-tetrahydroimidazo[1,2-a]pyridine-2-carboxamide
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PUGNAc-imidazole hybrid
2-((6S,7R,8R)-8-acetamido-6,7-dihydroxy-5-(hydroxymethyl)-5,6,7,8-tetrahydroimidazo[1,2-a]pyridin-2-yl)acetate
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NAGstatin
2-(acetylamino)-2-deoxy-1-S-hexanoyl-1-thio-beta-D-glucopyranose
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inhibits activity at 10 mM, 47.5% relative activity compared with activity without any addition of effector
2-acetamido-2-deoxy-D-gluconhydroxime-1,5-lactone
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inhibits activity at 0.1 mM, 6.7% relative activity compared with activity without any addition of effector
2-acetamindo-1-amino-1,2-dideoxy-beta-D-glucopyranose
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inhibits activity at 10 mM, 3.2% relative activity compared with activity without any addition of effector
2-acetamindo-1-azido-1,2-dideoxy-beta-D-glucopyranose
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inhibits activity at 10 mM, 19.3% relative activity compared with activity without any addition of effector
2-acetamindo-1-bromo-1,2-dideoxy-beta-D-glucopyranose
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inhibits activity at 10 mM, 34.8% relative activity compared with activity without any addition of effector
2-amino-1H-benzo[de]isoquinoline-1,3(2H)-dione
22.2% inhibition at 0.1 mM
2-[[1-[(2-acetamido-beta-D-glucopyranosylthiomethyl)-1H-1,2,3-triazol-4-yl]methyl]-1H-benzo[de]isoquinoline-1,3(2H)-dione
18.5% inhibition at 0.1 mM
2-[[1-[2-[(2-acetamido-beta-D-glucopyranosyl)thio]ethyl]-1H-1,2,3-triazol-4-yl]methyl]-1H-benzo[de]isoquinoline-1,3(2H)-dione
27.8% inhibition at 0.1 mM