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Information on EC 3.2.1.159 - alpha-neoagaro-oligosaccharide hydrolase

for references in articles please use BRENDA:EC3.2.1.159

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The enzyme appears in viruses and cellular organisms

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3.2.1.159

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alpha-neoagaro-oligosaccharide hydrolase

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Hydrolysis of the (1->3)-alpha-L-galactosidic linkages of neoagaro-oligosaccharides that are smaller than a hexamer, yielding 3,6-anhydro-L-galactose and D-galactose

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agarose degradation

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porphyran degradation

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alpha-neoagaro-oligosaccharide 3-glycohydrolase

When neoagarohexaose is used as a substrate, the oligosaccharide is cleaved at the non-reducing end to produce 3,6-anhydro-L-galactose and agaropentaose, which is further hydrolysed to agarobiose and agarotriose. With neoagarotetraose as substrate, the products are predominantly agarotriose and 3,6-anhydro-L-galactose. In Vibrio sp. the actions of EC 3.2.1.81, beta-agarase and EC 3.2.1.159 can be used to degrade agarose to 3,6-anhydro-L-galactose and D-galactose.

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60063-77-6

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Bacillus sp. (in: Bacteria)

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Manually annotated by BRENDA team

Bacillus sp. (in: Bacteria) MK03

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Manually annotated by BRENDA team

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Manually annotated by BRENDA team

Vibrio sp. JT0107

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Manually annotated by BRENDA team

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alpha-1,3-neoagarobiose + H2O

3,6-anhydro-L-galactose + D-galactose

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alpha-1,3-neoagarooligosaccharide + H2O

agaropentaose + agarotriose + agarobiose + D-galactose

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neoagarobiose + H2O

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neoagarohexaose + H2O

3,6-anhydro-L-galactose + agaropentaose

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neoagarotetraose + H2O

3,6-anhydro-L-galactose + agarotriose

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MgCl2

Bacillus sp. (in: Bacteria)

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2 mM, 163% of initial activity

SnCl2

Bacillus sp. (in: Bacteria)

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2 mM, 121% of initial activity

ZnCl2

Bacillus sp. (in: Bacteria)

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2 mM, 128% of initial activity

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AgNO3

Bacillus sp. (in: Bacteria)

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2 mM, 9% residual activity

CuCl2

Bacillus sp. (in: Bacteria)

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2 mM, 7% residual activity

EDTA

Bacillus sp. (in: Bacteria)

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2 mM, 47% residual activity

HgCl2

Bacillus sp. (in: Bacteria)

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2 mM, no residual activity

NiCl2

Bacillus sp. (in: Bacteria)

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2 mM, 10% residual activity

p-chloromercuribenzoate

Bacillus sp. (in: Bacteria)

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0.2 mM 60% residual activity

SDS

Bacillus sp. (in: Bacteria)

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2 mM, 53% residual activity

SrCl2

Bacillus sp. (in: Bacteria)

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2 mM, 43% residual activity

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5.37

neoagarobiose

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pH 7.7, 30°C

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22.2

Bacillus sp. (in: Bacteria)

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pH 7.0, 30°C

92

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pH 7.7, 30°C

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6.1

Bacillus sp. (in: Bacteria)

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7.7

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6.2 - 8.9

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drastical reduction in activity below or above

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4.6

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isoelectric focusing

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3p2n, download, 3D-view

Zobellia galactanivorans (strain DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij)

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42000

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84000

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gel filtration

320000

Bacillus sp. (in: Bacteria)

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gel filtration

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dimer

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octamer

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additional information

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6.8 - 8.6

Bacillus sp. (in: Bacteria)

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stable within this range

665765

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30

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40

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rapid loss of activity

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-20°C, phosphate buffer pH 7.8, 30% glycerol, 2 months, 60% residual activity

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4°C, phosphate buffer pH 7.8, 3 weeks, 60% residual activity, 2 months, 30% residual activity

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Show AA Sequence (2 entries)

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Show References (2 entries)

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NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM

IUBMB Enzyme Nomenclature

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)