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Information on EC 3.2.1.159 - alpha-neoagaro-oligosaccharide hydrolase for references in articles please use BRENDA:EC3.2.1.159Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The enzyme appears in viruses and cellular organisms
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alpha-neoagaro-oligosaccharide hydrolase
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Hydrolysis of the (1->3)-alpha-L-galactosidic linkages of neoagaro-oligosaccharides that are smaller than a hexamer, yielding 3,6-anhydro-L-galactose and D-galactose
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porphyran degradation
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alpha-neoagaro-oligosaccharide 3-glycohydrolase
When neoagarohexaose is used as a substrate, the oligosaccharide is cleaved at the non-reducing end to produce 3,6-anhydro-L-galactose and agaropentaose, which is further hydrolysed to agarobiose and agarotriose. With neoagarotetraose as substrate, the products are predominantly agarotriose and 3,6-anhydro-L-galactose. In Vibrio sp. the actions of EC 3.2.1.81, beta-agarase and EC 3.2.1.159 can be used to degrade agarose to 3,6-anhydro-L-galactose and D-galactose.
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alpha-1,3-neoagarobiose + H2O
3,6-anhydro-L-galactose + D-galactose
alpha-1,3-neoagarooligosaccharide + H2O
agaropentaose + agarotriose + agarobiose + D-galactose
neoagarohexaose + H2O
3,6-anhydro-L-galactose + agaropentaose
neoagarotetraose + H2O
3,6-anhydro-L-galactose + agarotriose
alpha-1,3-neoagarobiose + H2O
3,6-anhydro-L-galactose + D-galactose
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40% hydrolysis within 30 min
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alpha-1,3-neoagarobiose + H2O
3,6-anhydro-L-galactose + D-galactose
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40% hydrolysis within 30 min
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alpha-1,3-neoagarooligosaccharide + H2O
agaropentaose + agarotriose + agarobiose + D-galactose
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alpha-1,3-neoagarooligosaccharide + H2O
agaropentaose + agarotriose + agarobiose + D-galactose
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neoagarobiose + H2O
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neoagarobiose + H2O
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neoagarohexaose + H2O
3,6-anhydro-L-galactose + agaropentaose
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complete hydrolysis within 30 min
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neoagarohexaose + H2O
3,6-anhydro-L-galactose + agaropentaose
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complete hydrolysis within 30 min
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neoagarotetraose + H2O
3,6-anhydro-L-galactose + agarotriose
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complete hydrolysis within 30 min
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neoagarotetraose + H2O
3,6-anhydro-L-galactose + agarotriose
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complete hydrolysis within 30 min
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MgCl2
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2 mM, 163% of initial activity
SnCl2
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2 mM, 121% of initial activity
ZnCl2
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2 mM, 128% of initial activity
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AgNO3
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2 mM, 9% residual activity
CuCl2
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2 mM, 7% residual activity
EDTA
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2 mM, 47% residual activity
HgCl2
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2 mM, no residual activity
NiCl2
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2 mM, 10% residual activity
p-chloromercuribenzoate
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0.2 mM 60% residual activity
SDS
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2 mM, 53% residual activity
SrCl2
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2 mM, 43% residual activity
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5.37
neoagarobiose
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pH 7.7, 30°C
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6.2 - 8.9
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drastical reduction in activity below or above
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30
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4.6
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isoelectric focusing
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Zobellia galactanivorans (strain DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij)
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42000
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2 * 42000, SDS-PAGE
42000
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8 * 42000, SDS-PAGE
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dimer
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2 * 42000, SDS-PAGE
dimer
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2 * 42000, SDS-PAGE
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octamer
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8 * 42000, SDS-PAGE
octamer
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8 * 42000, SDS-PAGE
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additional information
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N-terminal amino acid sequence
additional information
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N-terminal amino acid sequence
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additional information
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N-terminal amino acid sequence
additional information
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N-terminal amino acid sequence
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6.8 - 8.6
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stable within this range
665765
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40
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rapid loss of activity
30
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stable up to, 30 min, 96% residual activity
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-20°C, phosphate buffer pH 7.8, 30% glycerol, 2 months, 60% residual activity
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4°C, phosphate buffer pH 7.8, 3 weeks, 60% residual activity, 2 months, 30% residual activity
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A0A238UBS7_9FLAO
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42705
TrEMBL
F0V1E3_ZOBGA
Zobellia galactanivorans (strain DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij)
408
46100
TrEMBL
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Sugano, Y.; Kodama, H.; Terada, I.; Yamazaki, Y.; Noma, M.
Purification and characterization of a novel enzyme, alpha-neoagarooligosaccharide hydrolase (alpha-NAOS hydrolase), from a marine bacterium, Vibrio sp. strain JT0107
J. Bacteriol.
176
6812-6818
1994
Vibrio sp., Vibrio sp. JT0107
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Suzuki, H.; Sawai, Y.; Suzuki, T.; Kawai, K.
Purification and characterization of an extracellular alpha-neoagarooligosaccharide hydrolase from Bacillus sp. MK03
J. Biosci. Bioeng.
93
456-463
2002
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) MK03
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