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Information on EC 3.2.1.157 - iota-carrageenase and Organism(s) Alteromonas macleodii and UniProt Accession Q9F5I8

for references in articles please use BRENDA:EC3.2.1.157
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EC Tree
IUBMB Comments
The main products of hydrolysis are iota-neocarratetraose sulfate and iota-neocarrahexaose sulfate. iota-Neocarraoctaose is the shortest substrate oligomer that can be cleaved. Unlike EC 3.2.1.81, beta-agarase and EC 3.2.1.83, kappa-carrageenase, this enzyme proceeds with inversion of the anomeric configuration. iota-Carrageenan differs from kappa-carrageenan by possessing a sulfo group on O-2 of the 3,6-anhydro-D-galactose residues, in addition to that present in the kappa-compound on O-4 of the D-galactose residues.
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This record set is specific for:
Alteromonas macleodii
UNIPROT: Q9F5I8
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Word Map
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The taxonomic range for the selected organisms is: Alteromonas macleodii
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
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4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-2-O-sulfonato-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-2-O-sulfonato-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhyd
Synonyms
iota-carrageenase, cgib_ce, cgi82a, cgia2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Endohydrolysis of (1->4)-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose-2-sulfate in iota-carrageenans
show the reaction diagram
inverting mechanism for the degradation of carrageenan by iota-carrageenases via single displacement reactions, overview
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
iota-carrageenan 4-beta-D-glycanohydrolase (configuration-inverting)
The main products of hydrolysis are iota-neocarratetraose sulfate and iota-neocarrahexaose sulfate. iota-Neocarraoctaose is the shortest substrate oligomer that can be cleaved. Unlike EC 3.2.1.81, beta-agarase and EC 3.2.1.83, kappa-carrageenase, this enzyme proceeds with inversion of the anomeric configuration. iota-Carrageenan differs from kappa-carrageenan by possessing a sulfo group on O-2 of the 3,6-anhydro-D-galactose residues, in addition to that present in the kappa-compound on O-4 of the D-galactose residues.
CAS REGISTRY NUMBER
COMMENTARY hide
74191-25-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
iota-carrageenan + H2O
iota-carrahexaose + iota-carratetraose
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
iota-carrageenan + H2O
iota-carrahexaose + iota-carratetraose
show the reaction diagram
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the enzyme belongs to the glycosyl hydrolase family 82, GH82, phylogenetic analysis of carrageenases from different groups, overview
additional information
residues R243, R303, and R353 are involved in substrate binding, residues E245, D247, E310, and H281 are catalytic residues. Glu245 acts as a catalytic proton donor in CgiA, and Asp247 acts as the general base that activates the catalytic water molecule. Three other residues are indirectly involved in the activity: Gln222 simultaneously binds the catalytic water molecule and a chloride ion, playing the essential function of structuring the water network in the active site. His281 participates in iota-carrageenan binding and is likely involved in proton trafficking with the proton donor Glu245, and Glu310 stabilizes the substrate intermediate conformation. Active site structure and molecular docking, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CGIA_ALTMA
491
0
54795
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
enzyme structure analysis and comparisons, overview
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
X-ray diffraction structure determination and analysis at 1.60 A resolution, PDB ID 1H80
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene cgiA, sequence comparisons and phylogenetic analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Zhu, B.; Ni, F.; Sun, Y.; Zhu, X.; Yin, H.; Yao, Z.; Du, Y.
Insight into carrageenases major review of sources, category, property, purification method, structure, and applications
Crit. Rev. Biotechnol.
38
1261-1276
2018
Alteromonas macleodii (Q9F5I8), Cellulophaga sp. QY3 (R9UQG9), Flavobacterium sp. YS-80-122 (A0A1W5QBK3), Microbulbifer thermotolerans (E3W9G3), Microbulbifer thermotolerans JAMB-A94T (E3W9G3), Wenyingzhuangia fucanilytica (A0A1B1Y261), Zobellia galactanivorans (D9UAT1), Zobellia galactanivorans (Q9F284), Zobellia galactanivorans CCUG 47099 (D9UAT1), Zobellia galactanivorans CCUG 47099 (Q9F284), Zobellia galactanivorans CIP 106680 (D9UAT1), Zobellia galactanivorans CIP 106680 (Q9F284), Zobellia galactanivorans Dsij (D9UAT1), Zobellia galactanivorans Dsij (Q9F284), Zobellia galactanivorans DSM 12802 (D9UAT1), Zobellia galactanivorans DSM 12802 (Q9F284), Zobellia galactanivorans NCIMB 13871 (D9UAT1), Zobellia galactanivorans NCIMB 13871 (Q9F284)
Manually annotated by BRENDA team