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Information on EC 3.2.1.157 - iota-carrageenase and Organism(s) Zobellia galactanivorans and UniProt Accession Q9F284

for references in articles please use BRENDA:EC3.2.1.157
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EC Tree
IUBMB Comments
The main products of hydrolysis are iota-neocarratetraose sulfate and iota-neocarrahexaose sulfate. iota-Neocarraoctaose is the shortest substrate oligomer that can be cleaved. Unlike EC 3.2.1.81, beta-agarase and EC 3.2.1.83, kappa-carrageenase, this enzyme proceeds with inversion of the anomeric configuration. iota-Carrageenan differs from kappa-carrageenan by possessing a sulfo group on O-2 of the 3,6-anhydro-D-galactose residues, in addition to that present in the kappa-compound on O-4 of the D-galactose residues.
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This record set is specific for:
Zobellia galactanivorans
UNIPROT: Q9F284
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Word Map
The taxonomic range for the selected organisms is: Zobellia galactanivorans
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-2-O-sulfonato-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhydro-2-O-sulfonato-alpha-D-galactopyranosyl-(1-3)-4-O-sulfonato-beta-D-galactopyranosyl-(1-4)-3,6-anhyd
Synonyms
iota-carrageenase, cgib_ce, cgi82a, cgia2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
iota-carrageenan 4-beta-D-glycanohydrolase (configuration-inverting)
The main products of hydrolysis are iota-neocarratetraose sulfate and iota-neocarrahexaose sulfate. iota-Neocarraoctaose is the shortest substrate oligomer that can be cleaved. Unlike EC 3.2.1.81, beta-agarase and EC 3.2.1.83, kappa-carrageenase, this enzyme proceeds with inversion of the anomeric configuration. iota-Carrageenan differs from kappa-carrageenan by possessing a sulfo group on O-2 of the 3,6-anhydro-D-galactose residues, in addition to that present in the kappa-compound on O-4 of the D-galactose residues.
CAS REGISTRY NUMBER
COMMENTARY hide
74191-25-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
iota-carrageenan + H2O
iota-carrahexaose + iota-carratetraose
show the reaction diagram
-
-
-
?
iota-carrageenan + H2O
?
show the reaction diagram
-
-
-
?
iota-carrageenan + H2O
iota-neocarratetraose sulfate + iota-neocarrahexaose sulfate
show the reaction diagram
-
endohydrolytic cleavage, the enzyme proceeds with an overall inversion of the anomeric configuration
major end products
-
?
additional information
?
-
-
the enzyme shows no activity with kappa-carrageenan, lambda-carrageenan, agar or agarose
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
iota-carrageenan + H2O
iota-carrahexaose + iota-carratetraose
show the reaction diagram
-
-
-
?
iota-carrageenan + H2O
?
show the reaction diagram
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the enzyme belongs to the glycosyl hydrolase family 82, GH82, phylogenetic analysis of carrageenases from different groups, overview
evolution
the enzyme belongs to the glycosyl hydrolase family 82, GH82, phylogenetic analysis of carrageenases from different groups, overview
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 50000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene cgiA, sequence comparisons and phylogenetic analysis
gene cgiA2, sequence comparisons and phylogenetic analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Barbeyron, T.; Michel, G.; Potin, P.; Henrissat, B.; Kloareg, B.
iota-Carrageenases constitute a novel family of glycoside hydrolases, unrelated to that of kappa-carrageenases
J. Biol. Chem.
275
35499-35505
2000
Zobellia galactanivorans
Manually annotated by BRENDA team
Zhu, B.; Ni, F.; Sun, Y.; Zhu, X.; Yin, H.; Yao, Z.; Du, Y.
Insight into carrageenases major review of sources, category, property, purification method, structure, and applications
Crit. Rev. Biotechnol.
38
1261-1276
2018
Alteromonas macleodii (Q9F5I8), Cellulophaga sp. QY3 (R9UQG9), Flavobacterium sp. YS-80-122 (A0A1W5QBK3), Microbulbifer thermotolerans (E3W9G3), Microbulbifer thermotolerans JAMB-A94T (E3W9G3), Wenyingzhuangia fucanilytica (A0A1B1Y261), Zobellia galactanivorans (D9UAT1), Zobellia galactanivorans (Q9F284), Zobellia galactanivorans CCUG 47099 (D9UAT1), Zobellia galactanivorans CCUG 47099 (Q9F284), Zobellia galactanivorans CIP 106680 (D9UAT1), Zobellia galactanivorans CIP 106680 (Q9F284), Zobellia galactanivorans Dsij (D9UAT1), Zobellia galactanivorans Dsij (Q9F284), Zobellia galactanivorans DSM 12802 (D9UAT1), Zobellia galactanivorans DSM 12802 (Q9F284), Zobellia galactanivorans NCIMB 13871 (D9UAT1), Zobellia galactanivorans NCIMB 13871 (Q9F284)
Manually annotated by BRENDA team