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Information on EC 3.2.1.154 - fructan beta-(2,6)-fructosidase and Organism(s) Beta vulgaris and UniProt Accession Q70XE6

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IUBMB Comments
Possesses one of the activities of EC 3.2.1.80, fructan beta-fructosidase. While the best substrates are the levan-type fructans such as 6-kestotriose [beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl alpha-D-glucopyranoside] and 6,6-kestotetraose [beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl alpha-D-glucopyranoside], some (but not all) inulin-type fructans can also be hydrolysed, but more slowly [cf. EC 3.2.1.153, fructan beta-(2,1)-fructosidase]. Sucrose, while being a very poor substrate, can substantially inhibit enzyme activity in some cases.
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Beta vulgaris
UNIPROT: Q70XE6
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The taxonomic range for the selected organisms is: Beta vulgaris
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
6-fructan exohydrolase, suc:fructan 6-fructosyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
fructan 6-exohydrolase
-
6-FEH
-
-
fructan 6-exohydrolase
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
-
SYSTEMATIC NAME
IUBMB Comments
(2->6)-beta-D-fructan fructohydrolase
Possesses one of the activities of EC 3.2.1.80, fructan beta-fructosidase. While the best substrates are the levan-type fructans such as 6-kestotriose [beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl alpha-D-glucopyranoside] and 6,6-kestotetraose [beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl alpha-D-glucopyranoside], some (but not all) inulin-type fructans can also be hydrolysed, but more slowly [cf. EC 3.2.1.153, fructan beta-(2,1)-fructosidase]. Sucrose, while being a very poor substrate, can substantially inhibit enzyme activity in some cases.
CAS REGISTRY NUMBER
COMMENTARY hide
1000597-62-5
-
37288-56-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6-kestose + H2O
?
show the reaction diagram
-
-
-
?
levan + H2O
?
show the reaction diagram
-
-
-
?
levansucrase + H2O
?
show the reaction diagram
Bacillus subtilis
-
-
?
sucrose + H2O
D-fructose + D-glucose
show the reaction diagram
-
-
-
?
1-kestose + H2O
?
show the reaction diagram
-
native enzyme shows 12% of the activity with levan
-
-
?
6-kestose + H2O
?
show the reaction diagram
-
native enzyme shows 77% of the activity with levan
-
-
?
levan + H2O
?
show the reaction diagram
-
-
-
-
?
levanbiose + H2O
beta-D-fructose
show the reaction diagram
-
native enzyme shows 63% of the activity with levan
-
-
?
neokestin + H2O
?
show the reaction diagram
-
native enzyme shows 25% of the activity with levan
-
-
?
neokestose + H2O
?
show the reaction diagram
-
native enzyme shows 93% of the activity with levan
-
-
?
phlein + H2O
?
show the reaction diagram
-
native enzyme shows 84% of the activity with levan
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
sucrose
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
77
6-kestose
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
6-kestose
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
134
sucrose
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.3 - 6.8
-
pH 4.3: about 65% of maximal activity, pH 6.8: about 40% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18 - 38
-
18°C: about 45% of maximal activity, 38°C: about 20% of maximal activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
6FEH_BETVU
606
1
68496
Swiss-Prot
Secretory Pathway (Reliability: 2)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F233D
introduction of acidic amino acid in vicinity of acid-base catalyst creating a beta-fructofuranosidase type of enzyme with dual activity against sucrose and levan, generally lower activities against levan and 6-kestose but conserving preference for these substrates over sucrose
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
heterologous expression in Pichia pastoris, pPICZalphaA vector
heterologous expression in Pichia pastoris
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Van den Ende, W.; De Coninck, B.; Clerens, S.; Vergauwen, R.; Van Laere, A.
Unexpected presence of fructan 6-exohydrolases (6-FEHs) in non-fructan plants: characterization, cloning, mass mapping and functional analysis of a novel 'cell-wall invertase-like' specific 6-FEH from sugar beet (Beta vulgaris L.)
Plant J.
36
697-710
2003
Beta vulgaris
Manually annotated by BRENDA team
Le Roy, K.; Lammens, W.; Van Laere, A.; Van den Ende, W.
Influencing the binding configuration of sucrose in the active sites of chicory fructan 1-exohydrolase and sugar beet fructan 6-exohydrolase
New Phytol.
178
572-580
2008
Beta vulgaris (Q70XE6), Beta vulgaris
Manually annotated by BRENDA team