The enzyme from Aspergillus aculeatus is specific for xyloglucan and does not hydrolyse other cell-wall components. The reaction involves endohydrolysis of 1,4-beta-D-glucosidic linkages in xyloglucan with retention of the beta-configuration of the glycosyl residues.
The enzyme from Aspergillus aculeatus is specific for xyloglucan and does not hydrolyse other cell-wall components. The reaction involves endohydrolysis of 1,4-beta-D-glucosidic linkages in xyloglucan with retention of the beta-configuration of the glycosyl residues.
XTH31 cleaves the substrate from about 186 kDa to about 6 kDa (median sizes) within 40 h. The enzyme does not have a preferred cleavage site close to either terminus
xyloglucan endotransglucosylase/hydrolases are a family of enzymes that mediate the construction and restructuring of xyloglucan cross-links, thereby controlling the extensibility or mechanical properties of the cell wall in a wide variety of plant tissues
assay method development and evaluation for realtime detection and in vivo measurement of enzyme activity in plant tissue, overview. Substrate is a fluorogenic resorufin beta-glycoside of a xylogluco-oligosaccharide, isolated from Tamarindus indica seed xyloglucans and synthesized as a specific substrate for in planta analysis of XEH activity
enzyme XTH15, a classical group-I/II xyloglucan endotransglucosylase/hydrolase (XTH), has high xyloglucan endotransglucosylase (XET) and undetectable xyloglucan hydrolase (XEH) activity in vitro
enzyme XTH15, a classical group-I/II xyloglucan endotransglucosylase/hydrolase (XTH), has high xyloglucan endotransglucosylase (XET) and undetectable xyloglucan hydrolase (XEH) activity in vitro
xyloglucan endotransglucosylase/hydrolases are a family of enzymes that mediate the construction and restructuring of xyloglucan cross-links, thereby controlling the extensibility or mechanical properties of the cell wall in a wide variety of plant tissues
enzyme XTH31 is strongly expressed in young, rapidly growing organs. XTH31 is more specialised, being expressed in fewer organs (24-h imbibed seeds, hypocotyl, root elongation zone, cotyledons and immature leaves), albeit at higher concentrations
enzyme XTH31 is strongly expressed in young, rapidly growing organs. XTH31 is more specialised, being expressed in fewer organs (24-h imbibed seeds, hypocotyl, root elongation zone, cotyledons and immature leaves), albeit at higher concentrations
enzyme XTH15 is strongly expressed in young, rapidly growing organs, but particularly in 24-h imbibed seeds, the seedling hypocotyl and root, and early-stage flowers (especially in the carpels)
enzyme XTH15 is strongly expressed in young, rapidly growing organs, but particularly in 24-h imbibed seeds, the seedling hypocotyl and root, and early-stage flowers (especially in the carpels)
the enzyme hydrolytic activity is essentially completely eliminated in AtXTH31/AtXTH32 double knockout lines. Despite the elimination of enzyme hydrolase activity in initiating root hairs in the xth31/xth32 mutant, root hair initiation nonetheless appears to be grossly unaffected
the enzyme hydrolytic activity is essentially completely eliminated in AtXTH31/AtXTH32 double knockout lines. Despite the elimination of enzyme hydrolase activity in initiating root hairs in the xth31/xth32 mutant, root hair initiation nonetheless appears to be grossly unaffected
xyloglucan endohydrolysis is a latent activity in the root cell wall remaining from the period of cell elongation (where, in particular, AtXTH31 transcript levels are high), which is not strictly essential for root hair initiation
a role for enzyme XTH31 in acid growth. Isozymes XTH15 and XTH31 are both strongly expressed in young, rapidly growing organs, suggesting that they play roles in cell expansion. In vitro, XTH15 has very high transglucanase (endotransglucosylase, XET) but undetectable hydrolytic activity (glucanase, XEH). In contrast, XTH31 has very high XEH activity and only slight XET activity
xyloglucan endohydrolysis is a latent activity in the root cell wall remaining from the period of cell elongation (where, in particular, AtXTH31 transcript levels are high), which is not strictly essential for root hair initiation
isozymes XTH15 and XTH31 are both strongly expressed in young, rapidly growing organs, suggesting that they play roles in cell expansion. In vitro, XTH15 has very high transglucanase (endotransglucosylase, XET) but undetectable hydrolytic activity (glucanase, XEH). In contrast, XTH31 has very high XEH activity and only slight XET activity
contruction of XTH31 single and double knockout lines with gene XTH32, the enzyme hydrolytic activity is essentially completely eliminated in AtXTH31/AtXTH32 double knockout lines
contruction of XTH31 single and double knockout lines with gene XTH32, the enzyme hydrolytic activity is essentially completely eliminated in AtXTH31/AtXTH32 double knockout lines
enzyme deletion mutant. Myzus persicae aphids settle preferentially on the mutant rather than on the wild-type. Ectopic expression of enzyme in the phloem is not sufficient to confer protection
atxth28: null mutant with insert blocking gene expression of AtXTH28, shorter proximal siliques with fewer seeds, reduced self-pollination, atxth28/AtXTH28: transgenic mutant with insertion of intact AtXTH28 fragment into atxth28 null-mutant, wild type silique growth and self-pollination ability restored, atxth27/atxth28: double-null-mutant, no additional phenotype expression compared to atxth28 null-mutant
construction of random XEG T-DNA insertion mutants, dozens of plant cell wall xeg mutants are identified, leading to the identification of 23 genetic loci that affect plant cell walls. One of the identified loci is XEG113, encoding a family 77 glycosyltransferase, GT77. Detailed analysis of the wall of this mutant indicates that its extensins, structural glyocoproteins present in walls, are underarabinosylated. Xeg-113 plants exhibit more elongated hypocotyls than wild-type, mutant cell wall composition and phenotype, overview
contruction of XTH31 single and double knockout lines with gene XTH32, the enzyme hydrolytic activity is essentially completely eliminated in AtXTH31/AtXTH32 double knockout lines
contruction of XTH31 single and double knockout lines with gene XTH32, the enzyme hydrolytic activity is essentially completely eliminated in AtXTH31/AtXTH32 double knockout lines
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene XTH31, phylogenetic analysis, Arabidopsis thaliana contains 33 phylogenetically diverse xyloglucan endo-transglycosylase/hydrolase (XTH) gene products, two of which, genes AtXTH31 and AtXTH32, are predominant xyloglucan endohydrolases due to clustering into group III-A, DNA and amino acid sequence determination and analysis
gene XTH32, phylogenetic analysis, Arabidopsis thaliana contains 33 phylogenetically diverse xyloglucan endo-transglycosylase/hydrolase (XTH) gene products, two of which, genes AtXTH31 and AtXTH32, are predominant xyloglucan endohydrolases due to clustering into group III-A, DNA and amino acid sequence determination and analysis
Distinct catalytic capacities of two aluminium-repressed Arabidopsis thaliana xyloglucan endotransglucosylase/hydrolases, XTH15 and XTH31, heterologously produced in Pichia
Phytochemistry
112
160-169
2015
no activity in Pichia pastoris, Arabidopsis thaliana (P93046), Arabidopsis thaliana (Q38911)