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Information on EC 3.2.1.151 - xyloglucan-specific endo-beta-1,4-glucanase
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EC Tree
3.2.1.151 xyloglucan-specific endo-beta-1,4-glucanaseIUBMB Comments
The enzyme from Aspergillus aculeatus is specific for xyloglucan and does not hydrolyse other cell-wall components. The reaction involves endohydrolysis of 1,4-beta-D-glucosidic linkages in xyloglucan with retention of the beta-configuration of the glycosyl residues.
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Word Map
- 3.2.1.151
- xyloglucans
- tamarind
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microfibrils
- xxxg
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expansins
-
wall-modifying
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hemicellulosic
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2.4.1.207
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mixed-linkage
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tropaeolum
- nasturtium
- agriculture
- synthesis
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myceliophthora
- degradation
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
xyloglucanase, xyloglucan endotransglucosylase/hydrolases, endoxyloglucanase, xeg12a, xeg5a, caxth1, mtxgh74, xeg74, cel74a, xcxgha, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
AfXEG74
AspGD
AtXTH31
AtXTH32
bacterial glycoside hydrolase family 74 endo-xyloglucanase
CJA_2477
CjGH74
endo-xyloglucanase
endoxyloglucanase
GH12 xyloglucanase
GH74 endo-xyloglucanase
GH74 xyloglucanase
Gly74A
LeXTH1
MtXgh74
MYCTH_116384
NXG1
oligoxyloglucan hydrolase
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PODO_RS11130
PoGH74
POXEG12A
PoxXEG12A
SaGH74A
SaGH74B
XcXGHA
XEG
XEG5
XEG74
XegA
XEH
XET
XG-ase
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Xgl74A
XH
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XTH
XTH3
xyloglucan endo-beta-1,4-glucanase
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xyloglucan endohydrolase
xyloglucan endotransglucosylase/hydrolase
xyloglucan endotransglucosylase/hydrolase protein 31
xyloglucan endotransglucosylase/hydrolase protein 32
xyloglucan endotransglycosylase/hydrolase
xyloglucan hydrolase
xyloglucan-specific beta-(1,4)-glucanase
xyloglucan-specific endo 1,4-beta-glucanase
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xyloglucan-specific endo-1,4beta-glucanase
xyloglucan-specific endo-beta-1,4-glucanase
xyloglucanase
xyloglucanendohydrolase
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xyloglycan hydrolase
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additional information
bacterial glycoside hydrolase family 74 endo-xyloglucanase
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bacterial glycoside hydrolase family 74 endo-xyloglucanase
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endoxyloglucanase
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XEG
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XTH
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xyloglucan endohydrolase
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xyloglucan endotransglucosylase/hydrolase
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xyloglucan endotransglucosylase/hydrolase
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xyloglucan endotransglycosylase/hydrolase
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splitting and reconnection of xyloglucan cross-links, role in self-pollination through stamen length control
xyloglucan endotransglycosylase/hydrolase
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xyloglucanase
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additional information
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the enzyme belongs to the glycohydrolase family 77
additional information
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XEG is a glycohydrolase GH12 family endo-glucanase
additional information
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the enzyme belongs to the glycohydrolase family 12
additional information
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the enzyme belongs to the glycohydrolase family 12
additional information
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the enzyme belongs to the glycoside hydrolase family 16, GH16
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
xyloglucan + H2O = 2 xyloglucan oligosaccharides
endohydrolysis of 1,4-beta-D-glucosidic linkages in xyloglucan
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis
hydrolysis of O-glycosyl bond
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transglycosylation
hydrolysis
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transglycosylation
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
[(1->6)-alpha-D-xylo]-(1->4)-beta-D-glucan glucanohydrolase
The enzyme from Aspergillus aculeatus is specific for xyloglucan and does not hydrolyse other cell-wall components. The reaction involves endohydrolysis of 1,4-beta-D-glucosidic linkages in xyloglucan with retention of the beta-configuration of the glycosyl residues.
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CAS REGISTRY NUMBER
COMMENTARY
76901-10-5
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O
2-chloro-4-nitrophenol + Glcbeta(1->4)Glcbeta(1->4)Glcbeta
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3.8% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
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-
?
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O
2-chloro-4-nitrophenol + Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
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100% activity
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?
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O
?
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?
2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
2-chloro-4-nitrophenol + ?
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1.3% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
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?
2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
?
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?
2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
2-chloro-4-nitrophenol + ?
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19.4% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
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?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
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?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
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?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
carboxyethyl cellulose + H2O
carboxyethyl cellulose oligosaccharides
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?
carboxymethyl curdlan + H2O
carboxymethyl curdlan oligosaccharides
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less than 2% of the activity with xyloglucan
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?
carboxymethyl pachyman + H2O
carboxymethyl pachyman oligosaccharides
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less than 2% of the activity with xyloglucan
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?
carob galactomannan + H2O
?
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4% activity compared to tamarind xyloglucan
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?
Glcbeta(1-3)Glcbeta(1-3)Glc + H2O
D-glucose + Glcbeta(1-3)Glc
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38% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
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?
Glcbeta(1-4)Glc(1-4)Glc + H2O
D-glucose + Glcbeta(1-4)Glc
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32% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
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?
Glcbeta(1-4)Glc(1-4)Glcbeta(1-4)Glc + H2O
Glcbeta(1-4)Glc
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76% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
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?
Glcbeta(1-4)Glc-2-chloro-4-nitrophenol + H2O
D-glucose + 2-chloro-4-nitrophenyl-beta-D-glucoside
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24% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
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?
Glcbeta(1-4)Glcbeta(1-3)Glc + H2O
D-glucose + Glcbeta(1-3)Glc
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52% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
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?
Glcbeta(1-4)Glcbeta(1-4)Glc(1-4)Glc-2-chloro-4-nitrophenol + H2O
Glcbeta(1-4)Glc(1-4)Glc + 2-chloro-4-nitrophenyl-beta-D-glucoside
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84% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
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?
Glcbeta(1-4)Glcbeta(1-4)Glc-2-chloro-4-nitrophenol + H2O
Glcbeta(1-4)Glc + 2-chloro-4-nitrophenyl-beta-D-glucoside
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68% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
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?
Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-3)Glc + H2O
Glcbeta(1-4)Glc + Glcbeta(1-3)Glc
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83% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
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?
Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc + H2O
Glcbeta(1-4)Glcbeta(1-4)Glc + GG
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?
Glcbeta(1->4)[Xylalpha(1->6)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
?
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?
hydroxyethyl cellulose + H2O
hydroxyethyl cellulose oligosaccharides
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?
tamarind seed xyloglucan + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc
tamarind seed xyloglucan + H2O
alpha-D-Xyl-(1->6)-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc (1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-[alpha-D-Xyl-(1->6)]-beta-D-Glc-beta-(1->4)-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-alpha-D-Xyl-(1->6)-beta-D-Glc-L-beta-(1->4)-D-Glc + alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-beta-(1->4)-D-Glc
tamarind xyloglucan + H2O
tamarind xyloglucooligosaccharides
XTH31 cleaves the substrate from about 186 kDa to about 6 kDa (median sizes) within 40 h. The enzyme does not have a preferred cleavage site close to either terminus
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?
wheat arabinoxylan + H2O
?
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15% activity compared to tamarind xyloglucan
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?
XXXG + H2O
XX + XG
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X stands for a substituted glucose residue, G for an unsubstituted glucose residue
270% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
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?
XXXGXXXG + H2O
XX + XGXX + XG
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X stands for a substituted glucose residue, G for an unsubstituted glucose residue
hydrolysis at both chain ends of substrate. 390% of the activity with Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glcbeta(1-4)Glc
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?
Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta + H2O
?
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?
Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
?
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?
xyloglucan heptasaccharide + H2O
?
tritium labeled
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?
xyloglucan oligosaccharide + H2O
?
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diverse substrates, substrate specificity, overview
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?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
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?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
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?
alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-D-Glc + H2O
?
Thermothelomyces thermophilus VKPM F-244
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?
barley beta-glucan + H2O
?
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lower activity compared to xyloglucan
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?
barley beta-glucan + H2O
?
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87% activity compared to tamarind xyloglucan
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?
barley beta-glucan + H2O
?
4% activity compared to tamarind seed xyloglucan
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?
barley beta-glucan + H2O
?
4% activity compared to tamarind seed xyloglucan
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?
barley beta-glucan + H2O
?
Thermothelomyces thermophilus VKPM F-244
4% activity compared to tamarind seed xyloglucan
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?
barley-beta-glucan + H2O
?
approximately 50fold lower specific activity for the natural mixed-linkage (1->3)/(1->4)-beta-glucan from barley compared to xyloglucan
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?
barley-beta-glucan + H2O
?
approximately 50fold lower specific activity for the natural mixed-linkage (1->3)/(1->4)-beta-glucan from barley compared to xyloglucan
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?
carboxymethyl cellulose + H2O
?
enzyme CjGH74 shows approximately 165fold lower specific activity for the artificial polysaccharide derivative hydroxyethyl cellulose compared to xyloglucan
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?
carboxymethyl cellulose + H2O
?
enzyme CjGH74 shows approximately 165fold lower specific activity for the artificial polysaccharide derivative hydroxyethyl cellulose compared to xyloglucan
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?
carboxymethyl cellulose + H2O
carboxymethyl cellulose oligosaccharides
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carboxymethyl cellulose-4M
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?
carboxymethyl cellulose + H2O
carboxymethyl cellulose oligosaccharides
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?
carboxymethyl cellulose + H2O
carboxymethyl cellulose oligosaccharides
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?
carboxymethylcellulose + H2O