Information on EC 3.2.1.144 - 3-deoxyoctulosonase

for references in articles please use BRENDA:EC3.2.1.144
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The expected taxonomic range for this enzyme is: Crassostrea virginica

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EC NUMBER
COMMENTARY hide
3.2.1.144
-
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RECOMMENDED NAME
GeneOntology No.
3-deoxyoctulosonase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-deoxyoctulosonyl-lipopolysaccharide + H2O = 3-deoxyoctulosonic acid + lipopolysaccharide
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
3-deoxyoctulosonyl-lipopolysaccharide hydrolase
Releases Kdo (alpha- and beta-linked 3-deoxy-D-manno-octulosonic acid) from different lipopolysaccharides, including Re-LPS from Escherichia coli and Salmonella, Rd-LPS from S. minnesota, and de-O-acyl-re-LPS. 4-Methylumbelliferyl-alpha-Kdo (alpha-Kdo-OMec) is also a substrate.
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CAS REGISTRY NUMBER
COMMENTARY hide
199128-67-1
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
oyster
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl 3-deoxy-alpha-D-manno-2-octulopyranosidonic acid + H2O
3-deoxy-D-manno-octulosonic acid + 4-methylumbelliferone
show the reaction diagram
-
-
-
?
re-lipopolysaccharide + H2O
3-deoxy-D-manno-octulosonic acid + lipopolysaccharide
show the reaction diagram
-
-
-
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
re-lipopolysaccharide + H2O
3-deoxy-D-manno-octulosonic acid + lipopolysaccharide
show the reaction diagram
-
-
-
?
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
-
pH-optimum for natural substrate re-lipopolysaccharide, optimum for 4-methylumbelliferyl 3-deoxy-alpha-D-manno-2-octulopyranosidonic acid at pH 3
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
top print hide
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 8
-
-
208531
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Sephacryl S200, SP-Fractogel, Con A-Sepharose, octyl-Sepharose
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LINKS TO OTHER DATABASES (specific for EC-Number 3.2.1.144)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)