The enzyme binds to chitin and randomly cleaves glycosidic linkages in chitin and chitodextrins in a non-processive mode, generating chitooligosaccharides and free ends on which exo-chitinases and exo-chitodextrinases can act. Activity is greatly stimulated in the presence of EC 1.14.99.53, lytic chitin monoxygenase, which attacks the crystalline structure of chitin and makes the polymer more accesible to the chitinase. cf. EC 3.2.1.202, endo-chitodextrinase.
chitinase, chitotriosidase, endochitinase, antifreeze protein, chit1, amcase, chitinase a, exochitinase, acidic mammalian chitinase, class i chitinase, more
The enzyme binds to chitin and randomly cleaves glycosidic linkages in chitin and chitodextrins in a non-processive mode, generating chitooligosaccharides and free ends on which exo-chitinases and exo-chitodextrinases can act. Activity is greatly stimulated in the presence of EC 1.14.99.53, lytic chitin monoxygenase, which attacks the crystalline structure of chitin and makes the polymer more accesible to the chitinase. cf. EC 3.2.1.202, endo-chitodextrinase.
chitinases are components of plant defence against higher concentrations of heavy metals. Accumulation of some isoforms in response to one but not to other metal ions suggests that these enzymes might also be involved in a more metal specific mechanism in affected plants
the double mutation affects the thermal stability and specific activities toward glycol chitin and chitin hexasaccharide compared to the wild-type enzyme, but the effect lies between the affection of the individual mutations
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-expression of the class II chitinase with type I ribosome inactivating protein in Brassica juncea via Agrobacterium tumefaciens strain EHA105-mediated transformation. The expression of chitinase and type I ribosome inactivating protein from a heterologous source in Brassica juncea provide subsequent protection against Alternaria leaf spot disease, phenotype, overview
due to the potential of broad-spectrum antifungal activity barley chitinase gene can be used to enhance fungal-resistance in crop plants such as rice, tobacco, tea and clover
the expression of chitinase and type I ribosome inactivating protein from a heterologous source in Brassica juncea provide subsequent protection against Alternaria leaf spot disease and can be helpful in increasing the production of Indian mustard
A flexible loop controlling the enzymatic activity and specificity in a glycosyl hydrolase family 19 endochitinase from barley seeds (Hordeum vulgare L.)
Dennhart, N.; Weigang, L.M.; Fujiwara, M.; Fukamizo, T.; Skriver, K.; Letzel, T.
26kDa endochitinase from barley seeds: real-time monitoring of the enzymatic reaction and substrate binding experiments using electrospray ionization mass spectrometry
Chhikara, S.; Chaudhury, D.; Dhankher, O.; Jaiwal, P.
Combined expression of a barley class II chitinase and type I ribosome inactivating protein in transgenic Brassica juncea provides protection against Alternaria brassicae